BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,030,972	C→G	6.8%	P343A (CCG→GCG)	PP_0887 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,030,972	0	C	G	6.8%	421.0 / 15.6	133	P343A (CCG→GCG)	PP_0887	sensor histidine kinase
Reads supporting (aligned to +/- strand): ref base C (54/70); new base G (4/5); total (58/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 137 total aligned reads displayed.

GCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAG  >  NC_002947/1030823‑1031116

gcgcAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGgcgc                                                                                                                                                   <  1:840724/150‑1 (MQ=255)

     gCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGcgccgccg                                                                                                                                              >  1:1693316/1‑150 (MQ=255)

      ccGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGcgccgccgc                                                                                                                                             >  1:1867612/1‑150 (MQ=255)

      ccGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGcgccgccgc                                                                                                                                             <  2:768412/150‑1 (MQ=255)

        gAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCa                                                                                                                                           >  1:2383876/1‑150 (MQ=255)

          aGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGa                                                                                                                                         <  1:1728325/150‑1 (MQ=255)

           gCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGAc                                                                                                                                        <  1:572271/150‑1 (MQ=255)

             cAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGAcgc                                                                                                                                      <  1:1143781/150‑3 (MQ=255)

             cAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGAcgc                                                                                                                                      <  1:775080/150‑3 (MQ=255)

              aaCCGGCGCCTGGCCATCGTGGAGCAGGATGTGAGCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTg                                                                                                                                     >  1:1377943/1‑150 (MQ=255)

              aaCCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTATTGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTg                                                                                                                                     >  1:626520/1‑150 (MQ=255)

                ccGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑Ac                                                                                                                                  <  2:573045/150‑1 (MQ=255)

                       cTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCg                                                                                                                           >  1:84677/1‑150 (MQ=255)

                        tGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGt                                                                                                                          >  2:1642262/1‑150 (MQ=255)

                          gCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTa                                                                                                                        >  2:828298/1‑150 (MQ=255)

                           ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑Acc                                                                                                                                 >  1:1339123/1‑140 (MQ=255)

                           ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑Acc                                                                                                                                 <  2:1339123/140‑1 (MQ=255)

                           ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                       <  1:1624140/150‑1 (MQ=255)

                           ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                       <  2:334087/150‑1 (MQ=255)

                           ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                       >  1:2095598/1‑150 (MQ=255)

                            cATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACGAAATCGTTACt                                                                                                                      >  1:1282679/1‑150 (MQ=255)

                            cATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                      <  1:1431708/150‑1 (MQ=255)

                                 tGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATc                                                                                                                 >  1:713255/1‑150 (MQ=255)

                                    aGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCGAACCTG‑ACCCAGGCGTTACTGAATctgc                                                                                                              <  1:1881740/150‑1 (MQ=255)

                                    aGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATctgc                                                                                                              <  2:709564/150‑1 (MQ=255)

                                      cAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATctgctg                                                                                                            >  1:199514/1‑150 (MQ=255)

                                         gATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAAc                                                                                                         >  2:2045517/1‑150 (MQ=255)

                                          aTGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑a                                                                                                                                   <  2:2234912/123‑1 (MQ=255)

                                          aTGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑a                                                                                                                                   >  1:2234912/1‑123 (MQ=255)

                                          aTGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACa                                                                                                        >  1:2081949/1‑150 (MQ=255)

                                            gtgACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGGAGGCTGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAAt                                                                                                      <  2:1640138/150‑1 (MQ=255)

                                              gcccGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgc                                                                                                    >  2:593548/3‑150 (MQ=255)

                                              gACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgc                                                                                                    <  2:2292559/150‑1 (MQ=255)

                                               aCCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGGCGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgcc                                                                                                   >  1:1693728/1‑150 (MQ=255)

                                               aCCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgcc                                                                                                   <  2:1900938/150‑1 (MQ=255)

                                                    ctggctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCTCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGa                                                                                              >  2:1259175/1‑150 (MQ=255)

                                                     tggctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCTTAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGAt                                                                                             <  1:624517/150‑1 (MQ=255)

                                                       gctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGAtgc                                                                                           <  2:1170088/150‑1 (MQ=255)

                                                       gctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAACCAGCTACCTTTTCCTGAGCATGCCCGACCGCAACGTGCCGCTCCTGGCCCCGCCGCCAGACCCG‑ACCCAGGCGTTACTGACTCTGCTGAACAATGCCGCCGAtgc                                                                                           <  2:1084940/150‑1 (MQ=255)

                                                          ggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGAtgcctg                                                                                        >  2:583667/1‑150 (MQ=255)

                                                              gAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCt                                                                                    >  2:1065636/1‑150 (MQ=255)

                                                               aGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtg                                                                                   <  2:1867612/150‑1 (MQ=255)

                                                                ggCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                      <  2:2092046/114‑1 (MQ=255)

                                                                ggCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgc                                                                                                    <  2:1975020/132‑1 (MQ=255)

                                                                    cTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACTCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTgatgat                                                                              <  2:2403199/150‑1 (MQ=255)

                                                                    cTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTgatgat                                                                              <  2:149597/150‑1 (MQ=255)

                                                                     tCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATc                                                                             <  2:84677/150‑1 (MQ=255)

                                                                     tCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATc                                                                             >  1:817631/1‑150 (MQ=255)

                                                                      cAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCt                                                                            >  1:2073081/1‑150 (MQ=255)

                                                                      cAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCt                                                                            <  1:1853266/150‑1 (MQ=255)

                                                                        aCCGCTGGCACCTGATGCGCCGGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTgg                                                                          <  2:859959/150‑1 (MQ=255)

                                                                        aCCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTgg                                                                          <  2:1857780/150‑1 (MQ=255)

                                                                          cGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGaa                                                                        >  2:629237/1‑150 (MQ=255)

                                                                            cTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                      >  1:619041/1‑102 (MQ=255)

                                                                            cTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGt                                                                      <  1:2240704/150‑1 (MQ=255)

                                                                             gatgtccTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGtt                                                                     >  2:396626/6‑150 (MQ=255)

                                                                                cACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGc                                                                  <  2:817632/150‑1 (MQ=255)

                                                                                cACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGc                                                                  <  2:817631/150‑1 (MQ=255)

                                                                                 aCCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGcc                                                                 >  1:701347/1‑150 (MQ=255)

                                                                                 aCCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGcc                                                                 <  2:1693316/150‑1 (MQ=255)

                                                                                     gATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGCCCTC‑CCCCAGGCGTTACTGAAGCTGCTGAACCATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                             <  2:1586533/150‑1 (MQ=255)

                                                                                     gATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                             <  2:2073081/150‑1 (MQ=255)

                                                                                     gATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                             >  1:2356363/1‑150 (MQ=255)

                                                                                     gATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACACAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                             <  2:1377943/150‑1 (MQ=255)

                                                                                         cgcCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTgg                                                         <  1:583667/150‑1 (MQ=255)

                                                                                           cccGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                       <  2:1320855/86‑1 (MQ=255)

                                                                                           cccGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                       >  1:1320855/1‑86 (MQ=255)

                                                                                             cGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtgat                                                                                 >  1:389179/1‑122 (MQ=255)

                                                                                              ggAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGc                                                    >  1:396870/1‑150 (MQ=255)

                                                                                                 aGCCAGCTACCGCTTCAAGCGCCTGCGCGGCGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCTTGGACTGGAACGCCCa                                                 >  2:1223389/1‑150 (MQ=255)

                                                                                                  gCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAg                                                <  2:396870/150‑1 (MQ=255)

                                                                                                   ccAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                  <  2:1068695/115‑1 (MQ=255)

                                                                                                   ccAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                  >  1:1068695/1‑115 (MQ=255)

                                                                                                   ccAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGACAAGGCTTGCTCCGCCGATACACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAgg                                               >  2:683820/1‑150 (MQ=255)

                                                                                                      gCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCGGCCGATGCCTGCCCTGATGATCGGGAAGTTCGCCTGGACTGGGACGCCCAGGACa                                            <  2:332943/150‑1 (MQ=255)

                                                                                                        tACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATc                                          <  1:396626/150‑1 (MQ=255)

                                                                                                          ccGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGaa                                                                        <  2:1579533/118‑1 (MQ=255)

                                                                                                          ccGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGaa                                                                        >  1:1579533/1‑118 (MQ=255)

                                                                                                          ccGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGt                                        <  1:1389825/150‑1 (MQ=255)

                                                                                                          ccGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGt                                        >  1:2191927/1‑150 (MQ=255)

                                                                                                              ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                  <  1:1167179/104‑1 (MQ=255)

                                                                                                              ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                  >  2:1167179/1‑104 (MQ=255)

                                                                                                              ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatc                                    >  1:1053212/1‑150 (MQ=255)

                                                                                                              ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatc                                    >  1:242868/1‑150 (MQ=255)

                                                                                                               tCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatca                                   <  2:713255/150‑1 (MQ=255)

                                                                                                                         gcgcGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGa                         <  1:1200514/150‑1 (MQ=255)

                                                                                                                             gACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCTCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCAt                     <  1:1065636/150‑1 (MQ=255)

                                                                                                                                ggCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                    <  2:2198881/148‑1 (MQ=255)

                                                                                                                                ggCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                    >  1:2198881/1‑148 (MQ=255)

                                                                                                                                ggCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                    >  1:952980/1‑148 (MQ=255)

                                                                                                                                      gTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGCCCATGGTCCTGgt            <  1:1887667/150‑1 (MQ=255)

                                                                                                                                          cgcgTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTAc        <  2:1282679/150‑1 (MQ=255)

                                                                                                                                           gcgTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTg                                                               <  2:228764/94‑1 (MQ=255)

                                                                                                                                           gcgTCTGGCGCCGCCGACCGAGCAGCGCAGAAGCG‑TACTGGATCGGGTGGTCAAGGCGGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTAcc       <  2:1453705/150‑1 (MQ=255)

                                                                                                                                             gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGCTCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgc     >  2:395459/1‑150 (MQ=255)

                                                                                                                                             gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                    >  1:1824195/1‑135 (MQ=255)

                                                                                                                                             gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                    <  2:1824195/135‑1 (MQ=255)

                                                                                                                                             gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGGTCGCCTGGACTGGGACGCCCAGGACAGCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgc     >  1:695031/1‑150 (MQ=255)

                                                                                                                                              tCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgcc    >  2:2240530/1‑150 (MQ=255)

                                                                                                                                                tGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgccag  >  2:110839/1‑150 (MQ=255)

GCGCAGCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAG  >  NC_002947/1030823‑1031116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`