BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,030,975	C→G	6.7%	P344A (CCG→GCG)	PP_0887 →	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,030,975	0	C	G	6.7%	416.4 / 14.8	133	P344A (CCG→GCG)	PP_0887	sensor histidine kinase
Reads supporting (aligned to +/- strand): ref base C (54/70); new base G (4/5); total (58/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 142 total aligned reads displayed.

GCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAGCCATTGC  >  NC_002947/1030828‑1031123

gCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGcgccgccg                                                                                                                                                     >  1:1693316/1‑150 (MQ=255)

 ccGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGcgccgccgc                                                                                                                                                    >  1:1867612/1‑150 (MQ=255)

 ccGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGcgccgccgc                                                                                                                                                    <  2:768412/150‑1 (MQ=255)

    aaGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAg                                                                                                                                                 <  2:1550046/150‑1 (MQ=255)

     aGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGa                                                                                                                                                <  1:1728325/150‑1 (MQ=255)

      gCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGAc                                                                                                                                               >  2:775080/1‑150 (MQ=255)

      gCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGAc                                                                                                                                               <  1:572271/150‑1 (MQ=255)

        cAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGAcgc                                                                                                                                             <  1:1143781/150‑3 (MQ=255)

        cAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGAcgc                                                                                                                                             <  1:775080/150‑3 (MQ=255)

         aaCCGGCGCCTGGCCATCGTGGAGCAGGATGTGAGCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTg                                                                                                                                            >  1:1377943/1‑150 (MQ=255)

         aaCCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGGCGGCGCCAGAcgcg                                                                                                                                            <  2:1884299/150‑4 (MQ=255)

           ccGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑Ac                                                                                                                                         <  2:573045/150‑1 (MQ=255)

                 ccTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGc                                                                                                                                   <  2:1052329/150‑1 (MQ=255)

                  cTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCg                                                                                                                                  >  1:84677/1‑150 (MQ=255)

                   tGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGt                                                                                                                                 >  2:1642262/1‑150 (MQ=255)

                     gCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTa                                                                                                                               >  2:828298/1‑150 (MQ=255)

                     gCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTa                                                                                                                               >  1:244225/1‑150 (MQ=255)

                      ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑Acc                                                                                                                                        <  2:1339123/140‑1 (MQ=255)

                      ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                              <  1:1624140/150‑1 (MQ=255)

                      ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                              <  2:1260567/150‑1 (MQ=255)

                      ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                              <  2:334087/150‑1 (MQ=255)

                      ccATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                              >  1:2095598/1‑150 (MQ=255)

                       cATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACGAAATCGTTACt                                                                                                                             >  1:1282679/1‑150 (MQ=255)

                       cATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                             <  1:1431708/150‑1 (MQ=255)

                       cATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                             >  1:2212273/1‑150 (MQ=255)

                            tGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATc                                                                                                                        >  1:713255/1‑150 (MQ=255)

                                    gATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAAc                                                                                                                >  2:2045517/1‑150 (MQ=255)

                                     aTGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑a                                                                                                                                          >  1:2234912/1‑123 (MQ=255)

                                     aTGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑a                                                                                                                                          <  2:2234912/123‑1 (MQ=255)

                                     aTGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACa                                                                                                               >  1:2081949/1‑150 (MQ=255)

                                       gtgACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGGAGGCTGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAAt                                                                                                             <  2:1640138/150‑1 (MQ=255)

                                         gcccGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgc                                                                                                           >  2:593548/3‑150 (MQ=255)

                                         gACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgc                                                                                                           <  2:2292559/150‑1 (MQ=255)

                                          aCCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgcc                                                                                                          <  2:1900938/150‑1 (MQ=255)

                                               ctggctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCTCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGa                                                                                                     >  2:1259175/1‑150 (MQ=255)

                                                tggctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCTTAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGAt                                                                                                    <  1:624517/150‑1 (MQ=255)

                                                  gctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGAtgc                                                                                                  <  2:1170088/150‑1 (MQ=255)

                                                  gctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGAtgc                                                                                                  <  1:1796008/150‑1 (MQ=255)

                                                  gctggACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAACCAGCTACCTTTTCCTGAGCATGCCCGACCGCAACGTGCCGCTCCTGGCCCCGCCGCCAGACCCG‑ACCCAGGCGTTACTGACTCTGCTGAACAATGCCGCCGAtgc                                                                                                  <  2:1084940/150‑1 (MQ=255)

                                                        cGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGccc                                                                                            <  2:572227/150‑1 (MQ=255)

                                                         gAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCt                                                                                           >  2:1065636/1‑150 (MQ=255)

                                                           ggCGGTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                         >  1:1857780/1‑150 (MQ=255)

                                                           ggCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                             <  2:2092046/114‑1 (MQ=255)

                                                           ggCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATgc                                                                                                           >  1:1975020/1‑132 (MQ=255)

                                                               cTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTgatgat                                                                                     <  2:149597/150‑1 (MQ=255)

                                                                tCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATc                                                                                    <  2:244225/150‑1 (MQ=255)

                                                                tCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATc                                                                                    >  1:817632/1‑150 (MQ=255)

                                                                tCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATc                                                                                    <  2:84677/150‑1 (MQ=255)

                                                                 cAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCt                                                                                   >  1:2073081/1‑150 (MQ=255)

                                                                 cAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCt                                                                                   <  1:1853266/150‑1 (MQ=255)

                                                                 cAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCt                                                                                   <  1:1259175/150‑1 (MQ=255)

                                                                   aCCGCTGGCACCTGATGCGCCGGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTgg                                                                                 <  2:859959/150‑1 (MQ=255)

                                                                   aCCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTgg                                                                                 >  2:1200514/1‑150 (MQ=255)

                                                                   aCCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTgg                                                                                 <  2:1857780/150‑1 (MQ=255)

                                                                       cTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                             <  2:619041/102‑1 (MQ=255)

                                                                       cTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACt                                                                                                                             >  1:619041/1‑102 (MQ=255)

                                                                        gatgtccTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGtt                                                                            >  2:396626/6‑150 (MQ=255)

                                                                           cACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGc                                                                         <  1:1642262/150‑1 (MQ=255)

                                                                           cACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGc                                                                         <  2:817632/150‑1 (MQ=255)

                                                                                gATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGCCCTC‑CCCCAGGCGTTACTGAAGCTGCTGAACCATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                                    <  2:1586533/150‑1 (MQ=255)

                                                                                gATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                                    >  1:2356363/1‑150 (MQ=255)

                                                                                    cgcCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTgg                                                                <  1:583667/150‑1 (MQ=255)

                                                                                     gcCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGGCGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTggg                                                               <  2:1693728/150‑1 (MQ=255)

                                                                                      cccGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTAc                                                                                                                              >  1:1320855/1‑86 (MQ=255)

                                                                                       ccGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGa                                                                    >  2:1103009/1‑143 (MQ=255)

                                                                                         ggAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGc                                                           >  1:396870/1‑150 (MQ=255)

                                                                                              ccAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                         >  1:1068695/1‑115 (MQ=255)

                                                                                              ccAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGACAAGGCTTGCTCCGCCGATACACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAgg                                                      >  2:683820/1‑150 (MQ=255)

                                                                                               cAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGa                                                     <  1:629237/150‑1 (MQ=255)

                                                                                               cAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACAATTTCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGa                                                     <  1:748080/150‑1 (MQ=255)

                                                                                                 gCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCGGCCGATGCCTGCCCTGATGATCGGGAAGTTCGCCTGGACTGGGACGCCCAGGACa                                                   <  2:332943/150‑1 (MQ=255)

                                                                                                   tACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATc                                                 <  1:396626/150‑1 (MQ=255)

                                                                                                     ccGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGaa                                                                               <  2:1579533/118‑1 (MQ=255)

                                                                                                     ccGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGt                                               >  1:2191927/1‑150 (MQ=255)

                                                                                                         ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCtga                                                                                         <  1:1167179/104‑1 (MQ=255)

                                                                                                         ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatc                                           >  1:1053212/1‑150 (MQ=255)

                                                                                                         ttCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatc                                           >  1:242868/1‑150 (MQ=255)

                                                                                                          tCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatca                                          <  2:713255/150‑1 (MQ=255)

                                                                                                            cAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCACCTGGACTGGGACGCCCAGGACATCGTCATCAGt                                        <  1:1406808/150‑1 (MQ=255)

                                                                                                                        gACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCTCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCAt                            <  1:1065636/150‑1 (MQ=255)

                                                                                                                         aCGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                           <  2:701347/150‑1 (MQ=255)

                                                                                                                                ggTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGaa                                                                               <  2:1342131/91‑1 (MQ=255)

                                                                                                                                ggTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTgg                    <  2:2095598/150‑1 (MQ=255)

                                                                                                                                ggTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCGGATGATCTGGaa                                                                               >  1:1342131/1‑91 (MQ=255)

                                                                                                                                 gTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGCCCATGGTCCTGgt                   <  1:1887667/150‑1 (MQ=255)

                                                                                                                                 gTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGgt                   <  2:199514/150‑1 (MQ=255)

                                                                                                                                     cgcgTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTAc               <  2:1282679/150‑1 (MQ=255)

                                                                                                                                      gcgTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTg                                                                      >  1:228764/1‑94 (MQ=255)

                                                                                                                                      gcgTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTg                                                                      <  2:228764/94‑1 (MQ=255)

                                                                                                                                      gcgTCTGGCGCCGCCGACCGAGCAGCGCAGAAGCG‑TACTGGATCGGGTGGTCAAGGCGGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTAcc              <  2:1453705/150‑1 (MQ=255)

                                                                                                                                        gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGCTCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgc            >  2:395459/1‑150 (MQ=255)

                                                                                                                                        gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATg                           <  2:1824195/135‑1 (MQ=255)

                                                                                                                                        gTCTGGCGGCGGCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGGTCGCCTGGACTGGGACGCCCAGGACAGCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgc            >  1:695031/1‑150 (MQ=255)

                                                                                                                                         tCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgcc           >  2:2240530/1‑150 (MQ=255)

                                                                                                                                           tGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCgccag         >  2:110839/1‑150 (MQ=255)

                                                                                                                                                  gccgccAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatca                                          >  1:2184780/1‑110 (MQ=255)

                                                                                                                                                  gccgccAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGtcatca                                          <  2:2184780/110‑1 (MQ=255)

                                                                                                                                                  gccgccAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAGCCATTGc  <  1:593548/150‑1 (MQ=255)

                                                                                                                                                  gccgccAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAGCCATTGc  >  1:2395979/1‑150 (MQ=255)

                                                                                                                                                  gccaccAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAGCCATTGc  >  2:474687/1‑150 (MQ=255)

GCCGAAGCCAACCGGCGCCTGGCCATCGTGGAGCAGGATGTGACCGCCTGGCTGGACGAGGCGCTCAACCGCTGGCACCTGATGCGCCCGGAAGCCAGCTACCGCTTCCAGCGCCTGCGCGACGGCAAGGTGCCGCGTCTGGCGCCGCCGCCAGACCTG‑ACCCAGGCGTTACTGAATCTGCTGAACAATGCCGCCGATGCCTGCCCTGATGATCTGGAAGTTCGCCTGGACTGGGACGCCCAGGACATCGTCATCAGTATCCACGACCATGGTCCTGGTGTACCGCCAGCCATTGC  >  NC_002947/1030828‑1031123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`