Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,041,945 T→G 9.1% V142G (GTT→GGT)  colR → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,041,9450TG 9.1% 250.1 / 17.3 99V142G (GTT→GGT) colRtwo‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base T (49/41);  new base G (4/5);  total (53/46)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.76e-01

GCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGCCTGCGCA  >  NC_002947/1041798‑1042094
                                                                                                                                                   |                                                                                                                                                     
gCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTa                                                                                                                                                     >  1:2049271/1‑150 (MQ=255)
gCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTa                                                                                                                                                     <  2:2179645/150‑1 (MQ=255)
gCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTACTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTa                                                                                                                                                     >  1:1465408/1‑150 (MQ=255)
  tCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTAcc                                                                                                                                                   >  1:2014996/1‑150 (MQ=255)
  tCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTAcc                                                                                                                                                   >  2:340010/1‑150 (MQ=255)
       ggCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCa                                                                                                                                              <  2:2033957/150‑1 (MQ=255)
       ggCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCa                                                                                                                                              <  2:2080520/150‑1 (MQ=255)
        gCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAg                                                                                                                                             <  2:1907613/150‑1 (MQ=255)
        gCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAg                                                                                                                                             <  2:471185/150‑1 (MQ=255)
         cTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAgg                                                                                                                                            <  1:601189/150‑1 (MQ=255)
          tggcgacTACCTGCGCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTACGCCGTGCCCAGGGCGGTGTGCGGCGCACGCAGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAggg                                                                                                                                           >  2:605348/4‑150 (MQ=255)
          tGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAggg                                                                                                                                           >  1:2346316/1‑150 (MQ=255)
          tGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAggg                                                                                                                                           >  1:1680095/1‑150 (MQ=255)
           gacgacTACCTGCTCAAGCCATTTGCCATGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGt                                                                                                                                          <  2:1270693/150‑1 (MQ=255)
              gacTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGTCTTACATGAGCTACTACGTCGATACCCTGGAGGTTACCCGCCAGGGgcgc                                                                                                                                       >  2:2410610/1‑150 (MQ=255)
                 tACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAGGGtatcga                                                                                                                                     <  2:1447868/149‑6 (MQ=255)
                 tACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAGGGtatcga                                                                                                                                     >  1:1447868/1‑144 (MQ=255)
                  aCCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTACTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGTCGGTTACCCGCCAGTGGCGccggg                                                                                                                                   >  1:499974/1‑147 (MQ=255)
                   cctcctCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGtt                                                                                                                                  <  1:231533/150‑1 (MQ=255)
                    cTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGcc                                                                                                                                      >  1:461361/1‑145 (MQ=255)
                    cTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGcc                                                                                                                                      <  2:461361/145‑1 (MQ=255)
                    cTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGt                                                                                                                                   <  2:1399196/148‑1 (MQ=255)
                    cTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGt                                                                                                                                   >  1:1399196/1‑148 (MQ=255)
                      gCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGGGCGACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGaa                                                                                                                               <  1:1023719/150‑1 (MQ=255)
                        tCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTg                                                                                                                                    <  1:1373407/143‑1 (MQ=255)
                        tCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGt                                                                                                                                   >  1:1197292/1‑144 (MQ=255)
                        tCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGt                                                                                                                                   <  2:1197292/144‑1 (MQ=255)
                        tCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTAGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTg                                                                                                                                    >  2:1373407/1‑143 (MQ=255)
                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAGGGtatcgagg                                                                                                                                   <  1:1805507/142‑8 (MQ=255)
                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAGGGtatcgagg                                                                                                                                   >  2:1805507/1‑135 (MQ=255)
                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTc                                                                                                                           >  2:451625/1‑150 (MQ=255)
                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTc                                                                                                                           >  2:1751985/1‑150 (MQ=255)
                            gCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATCCCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCaa                                                                                                                         <  2:1552117/150‑1 (MQ=255)
                            gCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCaa                                                                                                                         <  2:203583/150‑1 (MQ=255)
                                   gCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTc                                                                                                                  <  1:1118419/150‑1 (MQ=255)
                                   gCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTAGCCTGTTGAAGCTCAACCCgggg                                                                                                                  >  2:2396999/1‑148 (MQ=255)
                                      cTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGc                                                                                                               >  1:489820/1‑150 (MQ=255)
                                           ggAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCaa                                                                                                          >  2:2005536/1‑150 (MQ=255)
                                                tGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgctgc                                                                                                     <  1:2383420/150‑1 (MQ=255)
                                                   cggcgCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTgg                                                                                                  <  2:2346316/150‑1 (MQ=255)
                                                    ggcgCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGc                                                                                                 <  2:1051157/150‑1 (MQ=255)
                                                         gcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAGCCCGGTCGGCCTCAAGCTGCTGGCGGTAc                                                                                            <  1:2355990/150‑1 (MQ=255)
                                                         gcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTAc                                                                                            >  2:2196224/1‑150 (MQ=255)
                                                         gcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTAc                                                                                            <  2:2291545/150‑1 (MQ=255)
                                                           aTCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTg                                                                                          >  2:901630/1‑150 (MQ=255)
                                                            tCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGa                                                                                         >  1:2062900/1‑150 (MQ=255)
                                                            tCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGa                                                                                         >  1:2405301/1‑150 (MQ=255)
                                                            tCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGa                                                                                         >  1:2248690/1‑150 (MQ=255)
                                                              gAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATg                                                                                       >  2:2418361/1‑150 (MQ=255)
                                                               aaGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAggg                                                                                                                                           >  2:2420811/1‑97 (MQ=255)
                                                               aaGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAggg                                                                                                                                           <  1:2420811/97‑1 (MQ=255)
                                                                aGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCa                                                                                     <  1:758709/150‑1 (MQ=255)
                                                                       ctgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGtt                                                                                                                                  <  1:2210645/98‑1 (MQ=255)
                                                                       ctgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGtt                                                                                                                                  >  2:2210645/1‑98 (MQ=255)
                                                                       ctgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGc                                                                              <  2:2049271/150‑1 (MQ=255)
                                                                        tgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGc                                                                                                                                       <  1:1039958/92‑1 (MQ=255)
                                                                        tgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGc                                                                                                                                       >  2:1039958/1‑92 (MQ=255)
                                                                         gcgcCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTc                                                                                                                  <  1:2014452/112‑1 (MQ=255)
                                                                         gcgcCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTc                                                                                                                  >  2:2014452/1‑112 (MQ=255)
                                                                                   cAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCGCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTg                                                                  <  1:2396999/150‑1 (MQ=255)
                                                                                            gggCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAg                                                         <  1:2418361/150‑1 (MQ=255)
                                                                                                   gcACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACGCGCCAGGGGCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGCGGTCCTgg                                                  >  2:230671/1‑150 (MQ=255)
                                                                                                     aCCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGgaa                                                <  2:489820/150‑1 (MQ=255)
                                                                                                        cTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAg                                                                                                                              >  1:2091062/1‑69 (MQ=255)
                                                                                                        cTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAg                                                                                                                              <  2:2091062/69‑1 (MQ=255)
                                                                                                         tGCAGGTGGCTGACCTGAGCTACGACCTAGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGCACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGaagaag                                            >  2:1248227/1‑150 (MQ=255)
                                                                                                          gCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGACTGTTGAAGGACAACAAGGTCGGCCTCAAGATCCTGGCGGTACTGAGGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGc                                           <  2:1465408/150‑1 (MQ=255)
                                                                                                           cAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGc                                                     >  1:1622785/1‑139 (MQ=255)
                                                                                                           cAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGc                                                     <  2:1622778/139‑1 (MQ=255)
                                                                                                           cAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGc                                                     <  2:1622785/139‑1 (MQ=255)
                                                                                                           cAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGc                                                     >  1:1622778/1‑139 (MQ=255)
                                                                                                              gTGGCTGACCTGAGCTACGACCTCGCTGGCCTCGAGGTTACCCGCCAGGGTCGCCTGTTGCCGCTCAACCCGCTCGACCTCAACCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTtg                                       >  1:663389/1‑150 (MQ=255)
                                                                                                              gTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCACGCTGCTGGCGGTAGTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTtg                                       >  2:1302886/1‑150 (MQ=255)
                                                                                                                      ccTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGcga                               >  2:274394/1‑150 (MQ=255)
                                                                                                                       cTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCATAAAAGCCCGCACGTACTGCTCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGcgac                              <  1:1128212/150‑1 (MQ=255)
                                                                                                                       cTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGcgac                              <  1:2005536/150‑1 (MQ=255)
                                                                                                                       cTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGcgac                              >  2:1364256/1‑150 (MQ=255)
                                                                                                                       cTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGcgac                              >  1:1104834/1‑150 (MQ=255)
                                                                                                                            cTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCATTGTGGGGCGACGACTg                         >  1:1606042/1‑150 (MQ=255)
                                                                                                                              aCGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGAGGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGcc                       <  2:459040/150‑1 (MQ=255)
                                                                                                                               cGACCTCGGTACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGccc                      >  1:1888188/1‑150 (MQ=255)
                                                                                                                               cGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAAcc                                                                                                                       >  2:1484719/1‑53 (MQ=255)
                                                                                                                               cGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAAcc                                                                                                                       <  1:1484719/53‑1 (MQ=255)
                                                                                                                                gACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCt                     >  1:2042258/1‑150 (MQ=255)
                                                                                                                                gACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCt                     <  2:286835/150‑1 (MQ=255)
                                                                                                                                   cTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGAt                  >  2:231968/1‑150 (MQ=255)
                                                                                                                                   cTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGAt                  >  2:231998/1‑150 (MQ=255)
                                                                                                                                    tCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATa                 >  2:1534880/1‑150 (MQ=255)
                                                                                                                                    tCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATa                 <  1:1364256/150‑1 (MQ=255)
                                                                                                                                      gcgaCCCTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGc               >  1:2068693/4‑150 (MQ=255)
                                                                                                                                      gATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGc               >  2:2278715/1‑150 (MQ=255)
                                                                                                                                      gATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGc               <  2:2062900/150‑1 (MQ=255)
                                                                                                                                      gATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCACGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGc               >  2:1831099/1‑150 (MQ=255)
                                                                                                                                          cccTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACa           <  2:2068693/150‑1 (MQ=255)
                                                                                                                                          cccTGGAGGTTACCCGCCAGGGTCGCCTGTTGACGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACa           <  2:1772665/150‑1 (MQ=255)
                                                                                                                                          cccTGGAGGTTACCCGACAGGGTAGCCTGTTGAAGATCATCTAGGTCGAACCCTAGCGGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACa           <  2:1104834/150‑1 (MQ=255)
                                                                                                                                            cTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGc         >  2:1257800/1‑150 (MQ=255)
                                                                                                                                            cTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGc         >  1:872302/1‑150 (MQ=255)
                                                                                                                                             tGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGcc        >  1:885033/1‑150 (MQ=255)
                                                                                                                                                   ttACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGCCTGCGCa  >  1:921863/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGCCTGCGCA  >  NC_002947/1041798‑1042094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑