Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,240,064	A→T	13.7%	D220V (GAC→GTC)	PP_1080 →	molybdopterin‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,240,064	0	A	T	13.7%	171.4 / 17.8	66	D220V (GAC→GTC)	PP_1080	molybdopterin‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (30/26);  new base T (4/5);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

TGCTGATTCTTGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGCAGCAGGTGCGCGAGGCCA  >  NC_002947/1239917‑1240195                                                                                                                                                    |                                                                                                                                     tGCTGATTCTTGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑a                                                                                                                                   <  1:2346327/150‑1 (MQ=255) tGCTGATTCTTGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑a                                                                                                                                   <  2:809127/150‑1 (MQ=255)    tGATTCTTGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGcc                                                                                                                                >  2:2124903/1‑150 (MQ=255)        tCTTGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAttg                                                                                                                            <  1:1780967/150‑2 (MQ=255)          ttGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTTCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTgg                                                                                                                          >  1:165367/1‑149 (MQ=255)          ttGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAttgc                                                                                                                           <  1:1665572/149‑3 (MQ=255)          ttGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAttgc                                                                                                                           >  2:1665572/1‑147 (MQ=255)          ttGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGt                                                                                                                          >  1:1778012/1‑150 (MQ=255)                      cGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGtt                                                                                                                         <  2:999994/139‑1 (MQ=255)                      cGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGtt                                                                                                                         >  1:999994/1‑139 (MQ=255)                           gCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCggtggt                                                                                                         >  1:595997/1‑150 (MQ=255)                           gCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCggtggt                                                                                                         <  1:224215/150‑1 (MQ=255)                             ttCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCggtg                                                                                                           <  2:800838/146‑1 (MQ=255)                             ttCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCggtg                                                                                                           >  1:800838/1‑146 (MQ=255)                             ttCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATAGCTGAC‑ATCCATTTGTTCATTCTCTCCGGTGGTGa                                                                                                       >  1:526240/1‑150 (MQ=255)                                cAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑Ag                                                                                                                                  <  2:763819/120‑1 (MQ=255)                                cAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑Ag                                                                                                                                  >  1:763819/1‑120 (MQ=255)                                cAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGc                                                                                                    >  2:27888/1‑150 (MQ=255)                                 aaTGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGTGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCa                                                                                                   <  1:1195313/150‑1 (MQ=255)                                 aaTGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCa                                                                                                   >  1:1141554/1‑150 (MQ=255)                                 aaTGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCa                                                                                                   >  1:1628160/1‑150 (MQ=255)                                  aTGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTc                                                                                                                        >  2:2064077/1‑128 (MQ=255)                                  aTGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTc                                                                                                                        <  1:2064077/128‑1 (MQ=255)                                  aTGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAg                                                                                                  <  2:595997/150‑1 (MQ=255)                                             atGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGc                                                                                       >  2:1492922/1‑150 (MQ=255)                                             atGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCAGCACATTCTGAT‑AGCCATTGGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGc                                                                                       <  1:414335/150‑1 (MQ=255)                                                aCTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGTGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGt                                                                                                                          >  2:535597/1‑112 (MQ=255)                                                aCTGTTACGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGTGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGt                                                                                                                          <  1:535597/112‑1 (MQ=255)                                                        ggACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAttgcc                                                                                                                          <  1:2097448/104‑4 (MQ=255)                                                        ggACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAttgcc                                                                                                                          >  2:2097448/1‑101 (MQ=255)                                                            gTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAAcaca                                                                        >  2:409464/1‑150 (MQ=255)                                                               gagaAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAATACATTg                                                                     <  1:2037981/150‑1 (MQ=255)                                                               gagaAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTg                                                                     <  1:404254/150‑1 (MQ=255)                                                                    gcgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCTGGGGCGTAGCGAAACGGCGGCCATGAGTTAC‑AGCCATTCGTCCCCACGTCCCGGGGCTGATGCAGCCCTGATGTGCGGCTTGCTCAACACAGTGGTCgg                                                                >  2:1146404/1‑149 (MQ=255)                                                                    gcgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAtt                                                                                                                             <  1:2015570/89‑1 (MQ=255)                                                                    gcgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGTC‑AGCCAtt                                                                                                                             >  2:2015570/1‑89 (MQ=255)                                                                    gcgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGa                                                                >  2:184350/1‑150 (MQ=255)                                                                    gcgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAA‑CGCGGCCTCGACATTTGTTCATACGCCGCGGTGGTGATGCCGCCCTGTTGTGCGTCTTGCTCAACACATTGTTCGa                                                                >  1:1614858/1‑150 (MQ=255)                                                                     cgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGaa                                                               <  1:1492922/150‑1 (MQ=255)                                                                     cgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGaa                                                               <  2:2341107/150‑1 (MQ=255)                                                                     cgcCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCAGCGGCGTAGCGAAACGGCTGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGaa                                                               <  1:1334323/150‑1 (MQ=255)                                                                       ccTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAaga                                                             <  2:1628160/150‑1 (MQ=255)                                                                        cTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAagag                                                            >  2:80117/1‑150 (MQ=255)                                                                            aGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGcc                                                        <  1:2160268/150‑1 (MQ=255)                                                                                 cTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCa                                                   >  1:882655/1‑150 (MQ=255)                                                                                  tGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCAc                                                  >  2:1941045/1‑150 (MQ=255)                                                                                  tGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGATACAGGTGCAGCCCTGTTGTGCGGCTTGCGCAACACATTGTTCGAAGAGGGCCTGGCAc                                                  >  1:24264/1‑150 (MQ=255)                                                                                     gggCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACggg                                               <  1:184350/150‑1 (MQ=255)                                                                                      ggCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACgggg                                              <  2:882655/150‑1 (MQ=255)                                                                                            gggtggGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCa                                        <  2:2226391/149‑1 (MQ=255)                                                                                                       tggtggtggTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCa                             >  2:995880/1‑150 (MQ=255)                                                                                                          tggtggtTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACg                          <  1:1563956/150‑1 (MQ=255)                                                                                                             gggtTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGcc                       <  2:249582/149‑1 (MQ=255)                                                                                                               gtTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTg                     >  1:67414/1‑150 (MQ=255)                                                                                                                  gACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTgcag                  >  2:172515/1‑150 (MQ=255)                                                                                                                     ccGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCt                                                                                              >  1:926014/1‑71 (MQ=255)                                                                                                                     ccGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCt                                                                                              <  2:926178/71‑1 (MQ=255)                                                                                                                     ccGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCt                                                                                              <  2:926014/71‑1 (MQ=255)                                                                                                                     ccGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCt                                                                                              >  1:926178/1‑71 (MQ=255)                                                                                                                     ccGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGcagcag               >  1:1580274/1‑150 (MQ=255)                                                                                                                       gcggcgTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGCAGCAGGt             >  2:1041932/1‑150 (MQ=255)                                                                                                                             tAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCATGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGCAGCAGGTGCGCGa       <  1:2066990/150‑1 (MQ=255)                                                                                                                               gCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGCAGCAGGTGCGCGAgg     >  2:1003737/1‑150 (MQ=255)                                                                                                                                  aaaCGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGCAGCAGGTGCGCGAGGCCa  >  1:1287073/1‑150 (MQ=255)                                                                                                                                           aaaTGGCTGTC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCCGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTc                                           <  2:333986/99‑1 (MQ=255)                                                                                                                                           aaaTGGCTGTC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTc                                           >  1:333986/2‑100 (MQ=255)                                                                                                                                                    |                                                                                                                                     TGCTGATTCTTGGCGGCAACCCGCTGGCTTCCAATGGCAGCATCATGACTGTTCCGGACGTGGAGAAGCGCCTGAAGGCCCTGCGGGCGCGTGGTGGGCGCCTGGTGGTGGTTGACCCGCGGCGTAGCGAAACGGCGGCAATGGCTGAC‑AGCCATTTGTTCATACGCCCCGGTGGTGATGCAGCCCTGTTGTGCGGCTTGCTCAACACATTGTTCGAAGAGGGCCTGGCACGGGGCTCGCACTTGCCGGTCAACGGCCTGCAGCAGGTGCGCGAGGCCA  >  NC_002947/1239917‑1240195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑