BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,291,473	G→T	6.4%	L86L (CTG→CTT)	estC →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,291,473	0	G	T	6.4%	441.8 / 10.0	139	L86L (CTG→CTT)	estC	carboxylesterase
Reads supporting (aligned to +/- strand): ref base G (64/66); new base T (4/5); total (68/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 143 total aligned reads displayed.

GGTGGCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTGG  >  NC_002947/1291324‑1291617

ggtggCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTg                                                                                                                                                  <  2:2342149/150‑1 (MQ=255)

 gtggCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTgg                                                                                                                                                 <  2:564045/150‑1 (MQ=255)

   ggCGAAACCGTCGTTGCCCTGTGGGCCGGCAGTGCCGACAAGCACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCAGGcc                                                                                                                                               <  2:1117075/150‑1 (MQ=255)

      taaaCCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTc                                                                                                                                            <  1:439602/149‑1 (MQ=255)

      gAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCta                                                                                                                                            >  1:1453043/1‑149 (MQ=255)

      gAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTc                                                                                                                                            <  1:1989500/150‑1 (MQ=255)

      gAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTCCTCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTc                                                                                                                                            >  1:1729748/1‑150 (MQ=255)

        aaCCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCCAGCTTGCCCTCgc                                                                                                                                          <  1:2159843/150‑2 (MQ=255)

           cgtcgtTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGc                                                                                                                                       <  1:2453316/150‑1 (MQ=255)

           cgtcgtTGACCTGTGGGCCGGCAGTGCCAACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGc                                                                                                                                       >  2:1255970/1‑150 (MQ=255)

             tcgtTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGccc                                                                                                                                     <  1:502210/150‑1 (MQ=255)

                    ccTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGc                                                                                                                              >  2:1630866/1‑150 (MQ=255)

                     cTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGc                                                                                                                                                <  1:998061/131‑1 (MQ=255)

                     cTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGcc                                                                                                                             >  2:1010106/1‑150 (MQ=255)

                      tgtgGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGACAACGACGTTGCCCTCGATGCCCCGGTGGCCa                                                                                                                            >  1:1181464/1‑150 (MQ=255)

                              ggCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTgg                                                                                                                    <  2:1822961/150‑1 (MQ=255)

                              ggCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTgg                                                                                                                    <  1:1630866/150‑1 (MQ=255)

                              ggCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTgg                                                                                                                    <  2:116106/150‑1 (MQ=255)

                              ggCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTgg                                                                                                                    <  2:2094571/150‑1 (MQ=255)

                                  gTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCTAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCg                                                                                                                >  2:1699582/1‑150 (MQ=255)

                                       gACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTc                                                                                                           >  1:633775/1‑150 (MQ=255)

                                        aCAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCg                                                                                                          <  1:786552/150‑1 (MQ=255)

                                            ggACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCa                                                                                                      >  2:19681/1‑150 (MQ=255)

                                              aCGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAgg                                                                                                    <  2:584551/150‑1 (MQ=255)

                                               cGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGc                                                                                                   <  1:2082905/150‑1 (MQ=255)

                                                ggCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACATGCCCGAGTTCGCCCAGGCg                                                                                                  >  1:722777/1‑150 (MQ=255)

                                                 gCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCTAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCgg                                                                                                 <  1:1699582/150‑1 (MQ=255)

                                                  ccAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGccc                                                                                                                 >  1:1855461/1‑133 (MQ=255)

                                                  ccAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGccc                                                                                                                 <  2:1855461/133‑1 (MQ=255)

                                                      cagGCCTGGCACCGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACATTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAg                                                                                            <  1:233419/150‑1 (MQ=255)

                                                        ggCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCa                                                                                          <  1:652595/150‑1 (MQ=255)

                                                        ggCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCa                                                                                          <  1:1255970/150‑1 (MQ=255)

                                                        ggCCTGGAACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCa                                                                                          >  2:832304/1‑150 (MQ=255)

                                                        ggCCTCGTACAGCGCTACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCAGGTCCTCGCCC‑GTAGGTACGACGCGAGGGCAAGCTGGCCATCGATGCCCATGTGCTCACCTAGTTGCCCGAGTTCGCCCAGGCGGGTAAGCa                                                                                          <  1:2387854/150‑1 (MQ=255)

                                                          ccTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTg                                                                                                                     <  1:1013250/121‑1 (MQ=255)

                                                             ggCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGa                                                                                     >  2:1022009/1‑150 (MQ=255)

                                                             ggCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGa                                                                                     >  2:2277641/1‑150 (MQ=255)

                                                             ggCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGa                                                                                     <  1:1136621/150‑1 (MQ=255)

                                                                acaGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCa                                                                                  >  1:1821187/1‑150 (MQ=255)

                                                                 caGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCAc                                                                                 <  2:2372865/150‑1 (MQ=255)

                                                                    cGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCtt                                                                              <  2:478442/150‑1 (MQ=255)

                                                                    cGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGACCGCGTTGGTCCTGTTGCGGATGCAGGCCATCACCtt                                                                              <  2:1729748/150‑1 (MQ=255)

                                                                        aCCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGt                                                                          <  1:2183027/150‑1 (MQ=255)

                                                                         ccATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGc                                                                            <  1:1315923/147‑1 (MQ=255)

                                                                         ccATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCAACTTGc                                                                            >  2:1315923/1‑147 (MQ=255)

                                                                          cATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTc                                                                                                           <  1:2242086/115‑1 (MQ=255)

                                                                          cATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTaa                                                                                             <  2:366898/129‑1 (MQ=255)

                                                                          cATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTaa                                                                                             >  1:366898/1‑129 (MQ=255)

                                                                          cATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCa                                                                        >  1:202519/1‑150 (MQ=255)

                                                                          cATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCACTGCGCCCCTGCAAGCGCTGGTTCGCCCAGGCGGTGAAGCAGCCGATCACCTTGCGTcg                                                                        >  2:62688/1‑149 (MQ=255)

                                                                          cATCGCCAACCGGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTc                                                                                                           >  2:2242086/1‑115 (MQ=255)

                                                                                cAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGa                                                                                                               <  1:738218/105‑1 (MQ=255)

                                                                                cAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGa                                                                                                               >  2:738218/1‑105 (MQ=255)

                                                                                cAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAgctgct                                                                  >  2:1064688/1‑150 (MQ=255)

                                                                                 aaCCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTc                                                                 <  1:1775787/150‑1 (MQ=255)

                                                                                       ttCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCTCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCAc                                                           >  1:464483/1‑150 (MQ=255)

                                                                                       ttCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGAt                                                                                    <  1:1587505/125‑1 (MQ=255)

                                                                                       ttCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCAc                                                           >  2:221646/1‑150 (MQ=255)

                                                                                        tctcCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCAcc                                                          >  2:2377169/1‑150 (MQ=255)

                                                                                            cTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCa                                                                                          <  2:758942/114‑1 (MQ=255)

                                                                                            cTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGc                                                      >  1:487232/1‑150 (MQ=255)

                                                                                            cTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGc                                                      >  2:2019420/1‑150 (MQ=255)

                                                                                                  cAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGc                                                                                                                                       >  2:1122391/1‑63 (MQ=255)

                                                                                                  cAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCa                                                                        >  2:143251/1‑126 (MQ=255)

                                                                                                  cAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCtt                                                <  2:452830/150‑1 (MQ=255)

                                                                                                   aaGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTg                                               >  2:2444312/1‑150 (MQ=255)

                                                                                                       ccTTCGCGGCCGTTAC‑CGCCCTTCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCgg                                           >  1:1044738/1‑150 (MQ=255)

                                                                                                         ttCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCAAGCCGGca                                         <  2:1450074/150‑2 (MQ=255)

                                                                                                         ttCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCAAGCCGGca                                         >  1:1450074/1‑149 (MQ=255)

                                                                                                          tCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCAAGCCGGcac                                        <  2:290204/150‑3 (MQ=255)

                                                                                                            gcgGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGAAAGCTGGCCATCGATGAACCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCAtt                                      <  2:1707466/150‑1 (MQ=255)

                                                                                                              ggCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCg                                    >  2:294986/1‑150 (MQ=255)

                                                                                                               gCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGt                                   <  2:487232/150‑1 (MQ=255)

                                                                                                                 cGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGa                                 <  1:786176/150‑1 (MQ=255)

                                                                                                                 cGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGa                                 <  2:633775/150‑1 (MQ=255)

                                                                                                                      c‑cGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAgctgc                            >  2:2327622/1‑150 (MQ=255)

                                                                                                                      c‑cGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAgctgc                            <  1:2327622/150‑1 (MQ=255)

                                                                                                                          cccTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCtgctgc                         >  1:1737250/1‑150 (MQ=255)

                                                                                                                           ccTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGcc                        <  2:1769156/150‑1 (MQ=255)

                                                                                                                           ccTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGcc                        <  1:517212/150‑1 (MQ=255)

                                                                                                                            cTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCg                       <  1:202767/150‑1 (MQ=255)

                                                                                                                             tGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCgg                      <  1:294986/150‑1 (MQ=255)

                                                                                                                                aGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTCAGCAATCTATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTg                   >  2:566769/1‑150 (MQ=255)

                                                                                                                                  ttGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGTCGGCCATTCGTGAGCTGCTGCCGGCTGaa                 <  1:2277641/150‑1 (MQ=255)

                                                                                                                                  ttGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGaa                 <  1:1022009/150‑1 (MQ=255)

                                                                                                                                     gTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCtgctgc                         >  2:2034535/1‑139 (MQ=255)

                                                                                                                                     gTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGcc              >  1:1327656/1‑150 (MQ=255)

                                                                                                                                       cGGTGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGCTCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGGGAGCTGCTGCCGGCTGAAGCCtt            <  2:1181464/150‑1 (MQ=255)

                                                                                                                                       cGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCtt            <  1:333635/150‑1 (MQ=255)

                                                                                                                                        ggCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTg           <  1:2433447/150‑1 (MQ=255)

                                                                                                                                        atcGAGGGCCAGCTTGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTc                                                                         <  1:813567/86‑1 (MQ=255)

                                                                                                                                        atcGAGGGCCAGCTTGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTc                                                                         >  2:813567/3‑88 (MQ=255)

                                                                                                                                         gCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGt          <  1:19681/150‑1 (MQ=255)

                                                                                                                                             gggCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGa      >  2:1167158/1‑150 (MQ=255)

                                                                                                                                                cAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGaa                 >  1:1938079/1‑136 (MQ=255)

                                                                                                                                                 aaGCTGGCCCTCGCTGCCCCCGTGCCCGACCAGTGGCCCGAGTTGCCCCAGCCGGGTAAGCATTCGATCACCTTTCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTgg  <  2:146335/150‑1 (MQ=255)

                                                                                                                                                 aaGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTgg  >  1:310566/1‑150 (MQ=255)

                                                                                                                                                 aaGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTgg  <  1:1167158/150‑1 (MQ=255)

                                                                                                                                                 aaGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTgg  >  1:1867556/1‑150 (MQ=255)

                                                                                                                                                 aaGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCAAGCCGGcac                                        <  1:1374754/112‑3 (MQ=255)

GGTGGCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTAC‑CGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTGG  >  NC_002947/1291324‑1291617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`