BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,488,064	T→A	7.1%	K89M (AAG→ATG) ‡	degS ←	serine endoprotease DegS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,488,064	0	T	A	7.1%	300.5 / 10.8	100	K89M (AAG→ATG) ‡	degS	serine endoprotease DegS
Reads supporting (aligned to +/- strand): ref base T (49/43); new base A (4/3); total (54/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 101 total aligned reads displayed.

GTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCT  >  NC_002947/1487916‑1488211

gttgttGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCtt                                                                                                                                                     <  1:977929/150‑1 (MQ=255)

  tgttgGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGACCCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtgg                                                                                                                                                   <  1:1097205/150‑1 (MQ=255)

    ttgGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtggtg                                                                                                                                                 >  1:2044606/1‑150 (MQ=255)

      ggTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTggtggt                                                                                                                                               <  2:1389112/150‑1 (MQ=255)

        taagcagGTAGCCCTCCAGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGgt‑gt                                                                                                                                            >  2:1166225/3‑150 (MQ=255)

         cagtagGTAGCTCTCTGGGCTCATGATTACCGCCGAACTCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTa                                                                                                                                           <  2:495683/150‑1 (MQ=255)

           gcagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACa                                                                                                                                         >  2:1022160/1‑150 (MQ=255)

            cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAg                                                                                                                                        <  2:484999/150‑1 (MQ=255)

            cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAg                                                                                                                                        >  1:1869098/1‑150 (MQ=255)

            cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAg                                                                                                                                        <  1:948342/150‑1 (MQ=255)

              ggTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCTCTGTTTGGGCAGGTTATCGCCTAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGGTGGT‑GAACaaaa                                                                                                                                      <  2:1173646/150‑4 (MQ=255)

               gTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTggtggt                                                                                                                                               <  2:2292811/141‑1 (MQ=255)

               gTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTggtggt                                                                                                                                               >  1:2292811/1‑141 (MQ=255)

               gTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGtt                                                                                                                                     >  1:1081805/1‑150 (MQ=255)

               gTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTGATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGtt                                                                                                                                     >  2:1537346/1‑150 (MQ=255)

                    ccTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtggtg                                                                                                                                                 >  2:2052708/1‑134 (MQ=255)

                    ccTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTtggtg                                                                                                                                                 <  1:2052708/134‑1 (MQ=255)

                     ctctGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCAc                                                                                                                               >  1:2347453/1‑150 (MQ=255)

                           gCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCgg                                                                                                                         <  2:150238/150‑1 (MQ=255)

                           gCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCgg                                                                                                                         <  1:2087770/150‑1 (MQ=255)

                             tcgtGATTACAGCCGAACCCAGGCCTGATTCCCAACGGCGCTGCTTGGGCAGGTTATCGCCGAGGAACCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTAACCACCTTGGTGGT‑GTACAGGTTGGCCACCGGCgggg                                                                                                                       >  2:470700/4‑150 (MQ=255)

                              cATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGggc                                                                                                                      <  1:568377/150‑1 (MQ=255)

                                tGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGAAGGTTATCGCCGAAGACGAGGCGGAAATGAGGGTCTTCGAACAGCGGGTGGTCGCTGTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGggcgg                                                                                                                    <  2:1464282/150‑1 (MQ=255)

                                 gATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCAccgccg                                                                                                                          <  1:7855/143‑1 (MQ=255)

                                 gATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCAccgccg                                                                                                                          >  2:7855/1‑143 (MQ=255)

                                     aCCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGAggcgacnatc                                                                                                               <  2:1518722/150‑7 (MQ=255)

                                         ccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGGAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTcg                                                                                                           >  1:1196461/1‑149 (MQ=255)

                                          cgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAc                                                                                                          <  1:370206/150‑1 (MQ=255)

                                               ccAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGAGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgt                                                                                                     >  2:1035641/1‑150 (MQ=255)

                                                 aGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcg                                                                                                   >  2:215103/1‑150 (MQ=255)

                                                 aGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTT‑GCGCCCCTGGCGCTCGAACGGGTGGTCCACCCCCTGGCCGGCCAGGGCCAcggcgccg                                                                                                   >  2:1728996/1‑150 (MQ=255)

                                                  ggCTTGATTCCCAGCGGCGCTGATTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcgg                                                                                                  <  2:1201839/150‑1 (MQ=255)

                                                     ttGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcggcgt                                                                                               >  1:1591162/1‑150 (MQ=255)

                                                         ttCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGCACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGa                                                                                           <  1:1728996/150‑1 (MQ=255)

                                                         ttCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGa                                                                                           <  2:2044606/150‑1 (MQ=255)

                                                          tCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGac                                                                                          >  2:191996/1‑150 (MQ=255)

                                                            ccAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcg                                                                                                   >  2:2278534/1‑139 (MQ=255)

                                                            ccAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcg                                                                                                   <  1:2278534/139‑1 (MQ=255)

                                                             cAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGGCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACAcc                                                                                       >  1:2195235/1‑150 (MQ=255)

                                                              aGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCg                                                                                      <  1:321435/150‑1 (MQ=255)

                                                               gcggcgCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCgg                                                                                     <  2:1668640/150‑1 (MQ=255)

                                                               gcggcgCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCgg                                                                                     <  1:2271789/150‑1 (MQ=255)

                                                                     cTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTg                                                                               >  1:70857/1‑150 (MQ=255)

                                                                      tGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGc                                                                              <  1:1791286/150‑1 (MQ=255)

                                                                        tttGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCAt                                                                            <  2:1081805/150‑1 (MQ=255)

                                                                        tttGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGACAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCAt                                                                            >  1:1753125/1‑150 (MQ=255)

                                                                            ggCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATc                                                                        >  2:1547269/1‑150 (MQ=255)

                                                                               aGGTTATCGCCGAAGAAGCGGCGGTACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                     <  1:1524113/150‑1 (MQ=255)

                                                                               aGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGACACGGGGACCTGTTAGACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATGCgg                                                                     >  2:1401876/1‑150 (MQ=255)

                                                                               aGGTTACCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                     <  1:1826005/150‑1 (MQ=255)

                                                                                ggTTATCTCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGACAGGGTCACGGCGTCGGCGTACGACACCGGACCTTGCATGATCCggg                                                                    <  2:1225149/150‑1 (MQ=255)

                                                                                     tCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTc                                                               >  1:377528/1‑150 (MQ=255)

                                                                                        ccGAATAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgt                                                            >  2:822278/1‑150 (MQ=255)

                                                                                              aagCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCt                                                      <  2:1021452/150‑1 (MQ=255)

                                                                                                gcggcgGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtg                                                    >  1:2098172/1‑150 (MQ=255)

                                                                                                gcggcgGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtg                                                    <  1:114337/150‑1 (MQ=255)

                                                                                                   gcggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcg                                                                                                         >  2:1868727/1‑94 (MQ=255)

                                                                                                   gcggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcg                                                                                                         <  1:1868727/94‑1 (MQ=255)

                                                                                                     ggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCa                                               >  1:1433847/1‑150 (MQ=255)

                                                                                                     ggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCa                                               >  1:2040274/1‑150 (MQ=255)

                                                                                                      gAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAg                                              <  1:191996/150‑1 (MQ=255)

                                                                                                      gAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGACAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAg                                              <  2:1753125/150‑1 (MQ=255)

                                                                                                       aaCTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAgg                                             <  1:987778/150‑1 (MQ=255)

                                                                                                           gCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGa                                         >  2:1102122/1‑150 (MQ=255)

                                                                                                              ggTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGt                                      >  1:1252231/1‑150 (MQ=255)

                                                                                                               gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCggg                                                                    <  2:2043098/119‑1 (MQ=255)

                                                                                                               gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCggg                                                                    >  1:2043098/1‑119 (MQ=255)

                                                                                                               gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtg                                                    >  1:1746652/1‑135 (MQ=255)

                                                                                                               gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtg                                                    <  2:1746652/135‑1 (MQ=255)

                                                                                                               gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTc                                     >  1:2407833/1‑150 (MQ=255)

                                                                                                                tCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTcc                                    <  2:1252231/150‑1 (MQ=255)

                                                                                                                  ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctg                                  >  2:2125953/1‑150 (MQ=255)

                                                                                                                  ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctg                                  >  1:1220919/1‑150 (MQ=255)

                                                                                                                   tCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTACACTGTGGCACATCCGGCAGGTTGACGTCctgg                                 >  1:1945640/1‑150 (MQ=255)

                                                                                                                      aaCAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctg                              <  1:1537346/150‑1 (MQ=255)

                                                                                                                      aaCAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctg                              <  1:1547269/150‑1 (MQ=255)

                                                                                                                         aGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGc                           >  2:338910/1‑150 (MQ=255)

                                                                                                                          gCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTCCTGCAGGTTGTCGTCCTCGCTGGGCa                          >  1:1000571/1‑150 (MQ=255)

                                                                                                                             ggTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGcc                       <  2:2347453/150‑1 (MQ=255)

                                                                                                                                 ggCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGNGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGAGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAc                   >  1:599198/1‑150 (MQ=255)

                                                                                                                                  gcgcTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAcc                  >  1:697347/1‑150 (MQ=255)

                                                                                                                                      ttttGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt              >  1:2288800/1‑150 (MQ=255)

                                                                                                                                      ttttGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCAtt              >  2:136383/1‑150 (MQ=255)

                                                                                                                                       cctgtacaccacCAAGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGCGGACAGAGGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTg             >  1:1971581/7‑150 (MQ=255)

                                                                                                                                        ctgtacaccacCAAGGTGGT‑GAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGAc                                        >  1:1134441/6‑122 (MQ=255)

                                                                                                                                        ctgtacaccacCAAGGTGGT‑GAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGc            >  1:2185423/6‑150 (MQ=255)

                                                                                                                                        ctGTACACCACCAAGGTGGT‑GAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGAc                                        <  2:1134441/121‑1 (MQ=255)

                                                                                                                                         tgtacaccacCAAGGTGGT‑GAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCg                     <  2:418495/136‑1 (MQ=255)

                                                                                                                                         tGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCg           <  2:2195235/150‑1 (MQ=255)

                                                                                                                                         tGTACACCACCAAGGTGGT‑GAACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCg                     >  1:418495/1‑140 (MQ=255)

                                                                                                                                           ttcgacaccTTGGTGGT‑GTACAGGTTAGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGa         >  2:691265/6‑150 (MQ=255)

                                                                                                                                            tCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaa        <  1:230210/150‑1 (MQ=255)

                                                                                                                                              accaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTTTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaaaa      >  1:983179/1‑150 (MQ=255)

                                                                                                                                              accaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaaaa      <  1:2125953/150‑1 (MQ=255)

                                                                                                                                              accaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaaaa      >  2:891161/1‑150 (MQ=255)

                                                                                                                                               ccaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGTCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAccc                 >  2:1401495/1‑138 (MQ=255)

                                                                                                                                               ccaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTggg                            >  2:1983499/1‑127 (MQ=255)

                                                                                                                                               ccaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTggg                            <  1:1983499/127‑1 (MQ=255)

                                                                                                                                               ccaccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAccc                 <  1:1401495/138‑1 (MQ=255)

                                                                                                                                                  ccTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt  >  1:1754227/1‑150 (MQ=255)

GTTGTTGGTCAGCAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGT‑GTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCT  >  NC_002947/1487916‑1488211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`