Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,216,174 T→A 6.8% M1K (ATG→AAG) † PP_5493 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,216,1740TA 6.8% 226.8 / 7.0 73M1K (ATG→AAG) †PP_5493hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/39);  new base A (3/2);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

ACCCAGCTGGGATCATTCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAGGGCGCC  >  NC_002947/2216025‑2216319
                                                                                                                                                      |                                                                                                                                                 
acCCAGCTGGGATCATTCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAAt                                                                                                                                                   <  2:1711342/150‑1 (MQ=255)
  ccAGCTGGGATCATTCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATgc                                                                                                                                                 >  1:2050161/1‑150 (MQ=255)
         ggATCATTCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGcgcgccgc                                                                                                                                          <  2:367424/150‑1 (MQ=255)
           aTCATTCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAAATTAAGTGCAGAAGAAATGtgcgccgcgc                                                                                                                                        <  2:1731603/150‑1 (MQ=255)
               ttCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCtttt                                                                                                                                    >  1:1041513/1‑150 (MQ=255)
               ttCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCtttt                                                                                                                                    >  2:955923/1‑150 (MQ=255)
               ttCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCtttt                                                                                                                                    <  1:2440615/150‑1 (MQ=255)
               ttCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCtttt                                                                                                                                    >  1:2265414/1‑150 (MQ=255)
               ttCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCtttt                                                                                                                                    >  1:1337289/1‑150 (MQ=255)
                tCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTc                                                                                                                                   <  2:1002492/150‑1 (MQ=255)
                tCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTc                                                                                                                                   <  1:1820427/150‑1 (MQ=255)
                  gCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCgg                                                                                                                                 >  2:1374135/1‑150 (MQ=255)
                     ttAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGt                                                                                                                              <  2:1220497/150‑1 (MQ=255)
                       aGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTgg                                                                                                                            <  2:2050161/150‑1 (MQ=255)
                       aGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTgg                                                                                                                            <  1:1321469/150‑1 (MQ=255)
                           ggTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTaa                                                                                                                        <  2:1811250/150‑1 (MQ=255)
                               tctcACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGCGGTTAATGtt                                                                                                                    >  1:1285108/1‑150 (MQ=255)
                                 tcACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATgc                                                                                                                                                 <  2:473355/119‑1 (MQ=255)
                                 tcACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATgc                                                                                                                                                 >  1:473355/1‑119 (MQ=255)
                                 tcACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTAt                                                                                                                  >  1:1650321/1‑150 (MQ=255)
                                   acacGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATcc                                                                                                                >  2:1896516/1‑150 (MQ=255)
                                   acacGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATcc                                                                                                                <  2:2451482/150‑1 (MQ=255)
                                     acGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCt                                                                                                              <  2:2265414/150‑1 (MQ=255)
                                      aaataGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGGATGCATCTGAACTGATGTGCG‑TCGCCTGAGCAGAAGACTTCACAAGAGAATAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTc                                                                                                             >  1:543481/5‑150 (MQ=255)
                                          aGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGa                                                                                                         <  2:1337289/150‑1 (MQ=255)
                                           gTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGt                                                                                                                              <  1:116865/128‑1 (MQ=255)
                                           gTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGt                                                                                                                              >  2:116865/1‑128 (MQ=255)
                                               aTTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACt                                                                                                    <  1:1217627/150‑1 (MQ=255)
                                                 tCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTcc                                                                                                  >  2:2013169/1‑150 (MQ=255)
                                                        tttCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCt                                                                                           <  1:955923/150‑1 (MQ=255)
                                                             cAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCAATCAAGAAGATACCCTGCGAACATCt                                                                                      >  2:1134075/1‑150 (MQ=255)
                                                                tGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGc                                                                                   <  2:876696/150‑1 (MQ=255)
                                                                             cgaatgcgaTCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAgg                                                                      <  2:543481/150‑1 (MQ=255)
                                                                             cgaatgcgaTCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAgg                                                                      <  2:1041513/150‑1 (MQ=255)
                                                                               aatgcgaTCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCa                                                                    >  2:2192935/1‑150 (MQ=255)
                                                                                  gcgaTCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTa                                                                 <  1:2024065/150‑1 (MQ=255)
                                                                                              gCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCg                                                     <  2:1681564/150‑1 (MQ=255)
                                                                                               cATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTACGTGCCGAAGAAATTCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCgg                                                    <  2:1243962/150‑1 (MQ=255)
                                                                                                atatCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCggg                                                   <  2:228387/150‑1 (MQ=255)
                                                                                                   tCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCt                                                                                      >  2:773387/1‑112 (MQ=255)
                                                                                                   tCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCt                                                                                      <  1:773387/112‑1 (MQ=255)
                                                                                                     aaCTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACaa                                              >  1:1317014/1‑150 (MQ=255)
                                                                                                     aaCTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACaa                                              >  1:1732604/1‑150 (MQ=255)
                                                                                                     aaCTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGCTATCCCTCAAGAAAACTCCCCGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACaa                                              <  2:1324310/150‑1 (MQ=255)
                                                                                                      aCTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAgg                                                                      >  2:2178870/1‑125 (MQ=255)
                                                                                                      aCTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAgg                                                                      <  1:2178870/125‑1 (MQ=255)
                                                                                                        tGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACaaaaa                                           <  1:2192935/150‑1 (MQ=255)
                                                                                                             tgAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaaa                                      <  1:1896516/150‑1 (MQ=255)
                                                                                                              gAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAg                                     <  1:1602897/150‑1 (MQ=255)
                                                                                                                gTTTGATTCATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGtt                                   <  2:1317014/150‑1 (MQ=255)
                                                                                                                 tttGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaaa                                      >  2:207745/1‑146 (MQ=255)
                                                                                                                 tttGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaaa                                      <  1:207745/146‑1 (MQ=255)
                                                                                                                 tttGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaa                                       >  1:1031222/1‑145 (MQ=255)
                                                                                                                 tttGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaa                                       <  2:1031222/145‑1 (MQ=255)
                                                                                                                             gCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCggg                                                   <  1:1266660/121‑1 (MQ=255)
                                                                                                                             gCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCggg                                                   >  2:1266660/1‑121 (MQ=255)
                                                                                                                             gCA‑CCAACTTAAGTGAAGAAGAAAAGCGCGGCGCGCATTTc                                                                                                                                   <  1:232020/41‑1 (MQ=17)
                                                                                                                             gCA‑CCAACTTAAGTGAAGAAGAAAAGCGCGGCGCGCATTTc                                                                                                                                   >  2:232020/1‑41 (MQ=17)
                                                                                                                              cA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAgg                     >  1:1494493/1‑150 (MQ=255)
                                                                                                                                 ccAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCa                   >  2:938722/1‑150 (MQ=255)
                                                                                                                                           tGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTATGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGa         >  1:1455329/1‑150 (MQ=255)
                                                                                                                                            gaagaagaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAg        <  1:1703852/150‑1 (MQ=255)
                                                                                                                                            gaagaagaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTCGAg        <  2:1494493/150‑1 (MQ=255)
                                                                                                                                             aagaagaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAgg       <  2:1821934/150‑1 (MQ=255)
                                                                                                                                                aagaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAGGgcg    <  1:829003/150‑1 (MQ=255)
                                                                                                                                                aagaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAGGgcg    >  1:726420/1‑150 (MQ=255)
                                                                                                                                                 cgaaAAGCGCGGCGCGCATTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCg                                                     <  1:811896/99‑1 (MQ=255)
                                                                                                                                                 cgaaAAGCGCGGCGCGCATTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCg                                                     >  2:811896/2‑100 (MQ=255)
                                                                                                                                                 cgaaAAGCGCGGCGCGCATTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAGGgcgc   >  2:12200/2‑150 (MQ=255)
                                                                                                                                                  gaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCtt                                                                                     <  2:2430273/67‑1 (MQ=255)
                                                                                                                                                  gaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCtt                                                                                     >  1:2430273/1‑67 (MQ=255)
                                                                                                                                                  gaaATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAGGGCGcc  >  2:14542/1‑150 (MQ=255)
                                                                                                                                                   aaaTGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaaa                                      >  1:927893/1‑113 (MQ=255)
                                                                                                                                                   aaaTGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTaaa                                      <  2:927893/113‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
ACCCAGCTGGGATCATTCGCCTTAGGCGGTCTCTCACACGGCAGTGCATTCCACTATTTCCCAATGCTACAGTAATGCGAATGCGATCTGGTATGCATATCAACTGATGTGAGTTTGATTAATGAGCA‑CCAACTTAAGTGAAGAAGAAATGCGCGCCGCGCTTTTCGGAGTGGTTAATGTTATCCCTCAAGAAAACTCCCTGCGCTCATCTTGCAGTCCTGCTAAGGCAGTATCAAAAAACTCGGGGACAAAAATTAAAGTTACTTTGCACGTAGGCAACGAGTTTGAGGGCGCC  >  NC_002947/2216025‑2216319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑