BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,239,868	C→A	5.7%	V18V (GTC→GTA)	gltX →	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,239,868	0	C	A	5.7%	336.0 / 6.4	105	V18V (GTC→GTA)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (49/50); new base A (2/4); total (51/54)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 105 total aligned reads displayed.

ACAAGTGACGTTCGCGCCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCTGCGCTGGCTTGGCATCG  >  NC_002947/2239727‑2240016

aCAAGTGACGTTCGCGCCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTAGGCAGTGc                                                                                                                                               <  1:2187780/150‑1 (MQ=255)

  aaGTGACGTTCGCGCCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCt                                                                                                                                             >  2:410982/1‑150 (MQ=255)

   aGTGACGTTCGCGCCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTa                                                                                                                                            <  2:2382283/150‑1 (MQ=255)

        cGTTCGCGCCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTAGGCAGTGCCGAC‑Atgg                                                                                                                                      <  1:240260/150‑3 (MQ=255)

               gcCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGtt                                                                                                                               <  1:410982/150‑1 (MQ=255)

                ccATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTc                                                                                                                              <  2:786855/150‑1 (MQ=255)

                  aTTGCTGTAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCaa                                                                                                                            <  2:718072/150‑1 (MQ=255)

                  aTTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCaa                                                                                                                            <  2:676650/150‑1 (MQ=255)

                   ttGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAac                                                                                                                           <  1:735608/150‑1 (MQ=255)

                      cTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAactac                                                                                                                        <  1:1441205/150‑1 (MQ=255)

                       tGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAactact                                                                                                                       >  2:2083613/1‑150 (MQ=255)

                       tGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTAGGCAGTGCCGAC‑Atgggg                                                                                                                                    >  2:937928/1‑133 (MQ=255)

                       tGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTAGGCAGTGCCGAC‑Atgggg                                                                                                                                    <  1:937928/137‑5 (MQ=255)

                            cccGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCttt                                                                                                                  >  2:1694337/1‑150 (MQ=255)

                            cccGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGAACACCGTACGCATTTTTATTGC‑CAAGCAGCCGACGGGCTACCCCCTTTTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCttt                                                                                                                  <  1:1486617/150‑1 (MQ=255)

                                 tGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTgagaa                                                                                                             <  1:938214/150‑5 (MQ=255)

                                     cGCCTCCTGCTAACATCCCGCCTTTGTTTCCTATTGCTGTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAagcag                                                                                                         >  1:2410143/1‑150 (MQ=255)

                                      gCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAagcagc                                                                                                        >  1:1788135/1‑150 (MQ=255)

                                       cctcctGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAgcagca                                                                                                       <  1:1694337/150‑1 (MQ=255)

                                            tGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAagca                                                                                                          <  1:171438/142‑1 (MQ=255)

                                            tGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAagca                                                                                                          >  2:171438/1‑142 (MQ=255)

                                              cTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGt                                                                                                <  1:2060593/150‑1 (MQ=255)

                                              cTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGt                                                                                                <  1:208839/150‑1 (MQ=255)

                                              cTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGt                                                                                                <  2:616319/150‑1 (MQ=255)

                                                aaCATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGa                                                                                              <  2:1788135/150‑1 (MQ=255)

                                                   aTCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCg                                                                                                                                                      >  2:749720/1‑92 (MQ=255)

                                                   aTCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCg                                                                                                                                                      <  1:749720/92‑1 (MQ=255)

                                                    tCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTAGGCAGTGCCGAC‑At                                                                                                                                        >  1:858582/1‑104 (MQ=255)

                                                    tCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTAGGCAGTGCCGAC‑At                                                                                                                                        <  2:858582/104‑1 (MQ=255)

                                                     cccGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCa                                                                                         >  2:1360841/1‑150 (MQ=255)

                                                      ccGCCTTTGTCTAATCTCTCAATGGAGAGTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCAt                                                                                        >  2:585807/1‑150 (MQ=255)

                                                       cGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCTCGCTTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGCTCATc                                                                                       >  2:2171504/1‑150 (MQ=255)

                                                       cGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCttt                                                                                                                  >  2:1084695/1‑123 (MQ=255)

                                                       cGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCttt                                                                                                                  <  1:1084695/123‑1 (MQ=255)

                                                       cGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATc                                                                                       >  1:1830096/1‑150 (MQ=255)

                                                         ccTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGc                                                                                                                >  1:575001/1‑123 (MQ=255)

                                                         ccTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGc                                                                                                                <  2:575001/123‑1 (MQ=255)

                                                           tttGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTgc                                                                                   <  2:1794640/150‑1 (MQ=255)

                                                           tttGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTgc                                                                                   <  1:1360841/150‑1 (MQ=255)

                                                              gTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCa                                                                                >  2:647994/1‑150 (MQ=255)

                                                                ttCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATc                                                                              >  2:1142763/1‑150 (MQ=255)

                                                                 tCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCgg                                                                                                                                                     <  1:257995/79‑1 (MQ=255)

                                                                 tCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCgg                                                                                                                                                     >  2:257995/1‑79 (MQ=255)

                                                                  cATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATcc                                                                                      >  1:765356/1‑140 (MQ=255)

                                                                  cATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATcc                                                                                      <  2:765356/140‑1 (MQ=255)

                                                                   aTCTTTCGTTCGAGACTCTCCATGCCGTCCGTATGAGCGGGTGTTGGGCGATC‑ATCGCCCATCCACCCCCATGTCCGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGACAAGCAGCAAGGCGGTGAGTTCATCCGTCTCATCGaa                                                                           <  2:1106763/150‑1 (MQ=255)

                                                                   aTCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGaa                                                                           <  1:1257617/150‑1 (MQ=255)

                                                                   aTCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGaa                                                                           <  2:1830096/150‑1 (MQ=255)

                                                                      tttCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTgcg                                                                                  <  2:1944518/140‑1 (MQ=255)

                                                                      tttCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTgcg                                                                                  >  1:1944518/1‑140 (MQ=255)

                                                                      tttCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGac                                                                        >  1:1269836/1‑150 (MQ=255)

                                                                      tttCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGac                                                                        >  1:1137946/1‑150 (MQ=255)

                                                                       ttcgttcgAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCaa                                                                                                             >  1:1672475/1‑112 (MQ=255)

                                                                       ttcgttcgAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCaa                                                                                                             <  2:1672475/112‑1 (MQ=255)

                                                                        tcgttcgAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCACCTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGacac                                                                      <  2:912578/150‑1 (MQ=255)

                                                                          gttcgAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCg                                                                    >  2:2425757/1‑150 (MQ=255)

                                                                           ttcnAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGa                                                                   >  2:1508752/1‑150 (MQ=255)

                                                                           ttcgAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGa                                                                   >  1:293584/1‑150 (MQ=255)

                                                                            tcgAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGAt                                                                  <  1:302321/150‑1 (MQ=255)

                                                                              gagaCTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCa                                                                >  1:323260/1‑150 (MQ=255)

                                                                                gaCTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGc                                                              >  1:2424149/1‑150 (MQ=255)

                                                                                   tctcCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCAt                                                                                        <  2:1546905/121‑1 (MQ=255)

                                                                                   tctcCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCAt                                                                                        >  1:1546905/1‑121 (MQ=255)

                                                                                       cATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTc                                                                                                                              <  2:2028254/79‑1 (MQ=255)

                                                                                       cATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTc                                                                                                                              >  1:2028254/1‑79 (MQ=255)

                                                                                        aTGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTcc                                                      >  1:983503/1‑150 (MQ=255)

                                                                                             caccGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTgc                                                                                   >  2:1987914/1‑116 (MQ=255)

                                                                                             caccGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTgc                                                                                   <  1:1987914/116‑1 (MQ=255)

                                                                                             caccGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCAcgcg                                                 >  1:1546598/1‑150 (MQ=255)

                                                                                              tctgttcgcacgcGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGt                                                <  2:1450156/144‑1 (MQ=255)

                                                                                              accGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGCCCCCCAGGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCACCGAAGACACCGATCAGCTGCGCTCCACGCGt                                                <  1:1959141/150‑1 (MQ=255)

                                                                                              accGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCCCGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGt                                                <  2:808554/150‑1 (MQ=255)

                                                                                              accGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGaa                                                                           >  2:1058584/1‑123 (MQ=255)

                                                                                              accGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGaa                                                                           <  1:1058584/123‑1 (MQ=255)

                                                                                              accGTACGCACGCGTATTGCGCCATC‑GCCTACCGGAGCGCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGt                                                <  2:2410143/150‑1 (MQ=255)

                                                                                                  tACGCACGCGTATTGCGCCATC‑GCCTACCGGCGCCCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGt                                            <  1:2284569/150‑1 (MQ=255)

                                                                                                    cgcacgcGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCg                                          <  1:1320910/150‑1 (MQ=255)

                                                                                                    cgcacgcGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCg                                          <  2:293584/150‑1 (MQ=255)

                                                                                                     gcacgcGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTAAGGCgg                                         >  2:852595/1‑150 (MQ=255)

                                                                                                       acgcGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAg                                       >  2:645473/1‑150 (MQ=255)

                                                                                                          cgTATTGCGCCATC‑GCCTACCGGCGACCACCCTGCCGGCCACACGGACTACTACCCTCTT‑TACTACTGCTTTGCCAAGCAGCACGGCGGTGCGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCaa                                    <  2:1242164/150‑1 (MQ=255)

                                                                                                                 cgcCATC‑GCCTACCGGCGACCCCCATGTCGACACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACAGAAATGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCt                             >  1:1732515/1‑150 (MQ=255)

                                                                                                                  gcCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCACATACTGCTTTGCCAAGCAGGACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGGCGGAGCAACAGATCtt                            >  1:1490129/1‑150 (MQ=255)

                                                                                                                  gcCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCtt                            >  2:1486230/1‑150 (MQ=255)

                                                                                                                   ccATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTc                           <  1:1273851/150‑1 (MQ=255)

                                                                                                                    cATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCg                          >  2:877913/1‑150 (MQ=255)

                                                                                                                       c‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACg                       <  2:1269836/150‑1 (MQ=255)

                                                                                                                         gCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTGGTCCTGCGCATCGaa                                                                           >  1:147093/1‑97 (MQ=255)

                                                                                                                         gCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGaa                                                                           <  2:147093/97‑1 (MQ=255)

                                                                                                                          ccTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATAGCGGAAACGAATCAGCTTCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGcc                     >  2:663587/1‑150 (MQ=255)

                                                                                                                            tACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTgcgc                                                         >  1:1291110/1‑112 (MQ=255)

                                                                                                                            tACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTgcgc                                                         <  2:1291110/112‑1 (MQ=255)

                                                                                                                            tACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCt                   >  2:2152570/1‑150 (MQ=255)

                                                                                                                            tACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCt                   <  2:323260/150‑1 (MQ=255)

                                                                                                                               cGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCTgcg                >  2:1736189/1‑150 (MQ=255)

                                                                                                                               cGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCTgcg                >  1:1457168/1‑150 (MQ=255)

                                                                                                                                 gCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCTGCGCt              <  1:2425757/150‑1 (MQ=255)

                                                                                                                                      ccccATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGa                                              >  1:865553/1‑113 (MQ=255)

                                                                                                                                      ccccATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGa                                              <  2:865553/113‑1 (MQ=255)

                                                                                                                                             tCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCTGCGCTGGCTTGGCATCg  >  2:1761025/1‑150 (MQ=255)

ACAAGTGACGTTCGCGCCATTGCTGGAGCCCGGTGCCCGCCTCCTGCTAACATCCCGCCTTTGTTTCATCTTTCGTTCGAGACTCTCCATGACCACCGTACGCACGCGTATTGCGCCATC‑GCCTACCGGCGACCCCCATGTCGGCACTGCCTAC‑ATCGCCCTGTTCAACTACTGCTTTGCCAAGCAGCACGGCGGTGAGTTCATCCTGCGCATCGAAGACACCGATCAGCTGCGCTCCACGCGTGAGTCGGAGCAACAGATCTTCGACGCCCTGCGCTGGCTTGGCATCG  >  NC_002947/2239727‑2240016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`