Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,404,137 T→G 11.6% S241A (TCG→GCG) ‡ PP_2106 → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,404,1370TG11.6% 184.9 / 18.2 79S241A (TCG→GCG) ‡PP_2106ammonium transporter
Reads supporting (aligned to +/- strand):  ref base T (40/29);  new base G (4/5);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

GTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCG  >  NC_002947/2403989‑2404284
                                                                                                                                                    |                                                                                                                                                   
gTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTc                                                                                                                                                    <  2:2047293/150‑1 (MQ=255)
 tACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCg                                                                                                                                                   <  1:1493977/150‑1 (MQ=255)
  aCCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTTAGCGGCCTGGTGGCGATCAACTCGt                                                                                                                                                  <  1:440726/150‑1 (MQ=255)
     gcgATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGa                                                                                                                                               <  2:1386396/150‑1 (MQ=255)
     gcgATGGGCGATTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATACTGATCATCGGCTGGTTCGGCTTCAACGTCATGACTGCCGAAACCGTGCCGTACATCAGCGGCCGGGTGGCGATCAACCCGtaaa                                                                                                                                               >  2:500539/1‑147 (MQ=255)
        aTGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATgg                                                                                                                                            >  2:1630786/1‑150 (MQ=255)
           ggCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCa                                                                                                                                         <  2:805833/150‑1 (MQ=255)
            gCGTTTGGTGGCTTTTGCGCCATCGGGTATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAt                                                                                                                                        >  1:64593/1‑150 (MQ=255)
             cGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCacc                                                                                                                                       <  2:2249578/150‑3 (MQ=255)
                 tggtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                                   <  1:386893/150‑1 (MQ=255)
                 tgctggCTTTTGCGCCATCGAGCATTCCCTTTCTCGCGCTGGGCTCGTGGATCCTCATCATCTGCTGCTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGCGCGTCAGCGGCCTTGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                                   <  2:450446/147‑1 (MQ=255)
                   gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGGGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                 <  2:799217/150‑1 (MQ=255)
                   gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                 <  1:650302/150‑1 (MQ=255)
                   gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGAGCCTCATCATCGGCTGGTTCGGCTTCAACGTTATGAGTGCCCAGACCTTGCAGTGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                 <  2:1230054/150‑1 (MQ=255)
                   gtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGAGCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                                 <  2:64593/150‑1 (MQ=255)
                      gCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGc                                                                                                                              >  1:935013/1‑150 (MQ=255)
                        ttttGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCAc                                                                                                                            >  2:904624/1‑150 (MQ=255)
                           tGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCt                                                                                                                         >  1:31942/1‑150 (MQ=255)
                              gcCATCGAGCATTCCCTTTCTGGCGCTGCGCTCGTGGATCCTCATCATCGGCTGGTTCGTCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTAATGCGCATGGTCGCGTGCACCCTGTc                                                                                                                      >  2:226602/1‑150 (MQ=255)
                                cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAg                                                                                                                    >  1:578593/1‑150 (MQ=255)
                                cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                        <  2:221543/130‑2 (MQ=255)
                                cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                        >  1:221543/1‑129 (MQ=255)
                                  tCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGAtc                                                                                                                                             >  1:611684/1‑122 (MQ=255)
                                  tCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGAtc                                                                                                                                             <  2:611684/123‑2 (MQ=255)
                                     aGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTg                                                                                                               >  1:797731/1‑150 (MQ=255)
                                        aTTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGGCCAGACCTTGCAGGGCGTCAGCGGCCTTGTGGCGATCAACTCGTTGATGTGCATGGTGGGTGGCACCCTGTAAGCGTTGCTg                                                                                                            >  1:144172/1‑150 (MQ=255)
                                        aTTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTg                                                                                                            <  1:1183646/150‑1 (MQ=255)
                                         ttCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggtg                                                                                                                                >  2:1849519/1‑129 (MQ=255)
                                         ttCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggtg                                                                                                                                <  1:1849519/129‑1 (MQ=255)
                                          tCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGc                                                                                                          <  1:904624/150‑1 (MQ=255)
                                           cccTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGcc                                                                                                         >  2:1695317/1‑150 (MQ=255)
                                            ccTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCg                                                                                                        >  2:1021867/1‑150 (MQ=255)
                                             cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                       >  1:1460381/1‑150 (MQ=255)
                                             cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                       >  2:199036/1‑150 (MQ=255)
                                             cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                       >  1:2054350/1‑150 (MQ=255)
                                                                  gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                                  <  1:1630786/150‑1 (MQ=255)
                                                                  gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                                  <  2:935013/150‑1 (MQ=255)
                                                                        catcatCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                              >  2:998138/1‑132 (MQ=255)
                                                                        catcatCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                              <  1:998138/132‑1 (MQ=255)
                                                                          tcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCaccag                                                                                                                                     <  1:1642270/91‑5 (MQ=37)
                                                                          tcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCAACGAGTTGATCGCCaccag                                                                                                                                     >  2:1642270/1‑87 (MQ=37)
                                                                             tcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGcc                                                                       >  2:755895/1‑150 (MQ=255)
                                                                                gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                                    >  2:1052881/1‑150 (MQ=255)
                                                                                gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                                    >  1:1500429/1‑150 (MQ=255)
                                                                                         gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                           >  1:1934545/1‑150 (MQ=255)
                                                                                         gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                           >  2:549063/1‑150 (MQ=255)
                                                                                             cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                       <  2:1460381/150‑1 (MQ=255)
                                                                                             cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                       <  2:578593/150‑1 (MQ=255)
                                                                                             cAACGTCATGAGTGCCCAGACCTTGCAGGGCGTAAGTGGCCTGGTGGGGTTCAACTCGTTTTTGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtggt                                                       <  2:144172/150‑1 (MQ=255)
                                                                                                 gTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCg                                                                     <  1:2172440/132‑1 (MQ=255)
                                                                                                 gTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCg                                                                     >  2:2172440/1‑132 (MQ=255)
                                                                                                     tGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                               >  1:1977459/1‑150 (MQ=255)
                                                                                                        gTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTgcg                                            <  2:2054350/150‑1 (MQ=255)
                                                                                                        gTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTgcg                                            >  1:1156678/1‑150 (MQ=255)
                                                                                                           cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                         >  1:246374/1‑150 (MQ=255)
                                                                                                           cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                         >  1:246403/1‑150 (MQ=255)
                                                                                                           cccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAg                                         >  2:89018/1‑150 (MQ=255)
                                                                                                            ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGc                                                                                >  2:834007/1‑110 (MQ=255)
                                                                                                            ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGc                                                                                <  1:834007/110‑1 (MQ=255)
                                                                                                            ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGAGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCCGATTTTCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttttgcgcgg                                        >  1:1984149/1‑143 (MQ=255)
                                                                                                             cAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGt                                       >  2:1577887/1‑150 (MQ=255)
                                                                                                             cAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAAGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGt                                       >  2:1578738/1‑150 (MQ=255)
                                                                                                                  cTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGa                                  <  1:89018/150‑1 (MQ=255)
                                                                                                                  cTTGCAGGGCGTCAGCGGCCTGGTGGCGAGCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGa                                  >  1:1640315/1‑150 (MQ=255)
                                                                                                                         ggCGTCAGCGGCCTGGTGGCGATCAACTCGTTGACGGCGATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATg                           <  1:500539/150‑1 (MQ=255)
                                                                                                                           cGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGAGGCGATTTGCGCAGGTTCCGATCTGATGCa                         >  2:1279426/1‑150 (MQ=255)
                                                                                                                               aGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCggg                                                            >  2:529451/1‑111 (MQ=255)
                                                                                                                               aGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCggg                                                            <  1:529451/111‑1 (MQ=255)
                                                                                                                                     cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt               <  1:1577887/150‑1 (MQ=255)
                                                                                                                                     cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAAGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt               <  1:1578738/150‑1 (MQ=255)
                                                                                                                                      tggtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggtg              >  2:902354/1‑150 (MQ=255)
                                                                                                                                      tggtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTGTCTGCATGACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggtg              >  1:1738712/1‑150 (MQ=255)
                                                                                                                                           gCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTc         >  2:1481051/1‑150 (MQ=255)
                                                                                                                                            ccaTCAACGAGTTGATCGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCg        >  2:646654/3‑150 (MQ=255)
                                                                                                                                             gATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGc       >  2:994949/1‑150 (MQ=255)
                                                                                                                                               tCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGccc     >  2:2127159/1‑149 (MQ=255)
                                                                                                                                               tCAACTCGTTGATGGCCATGGTGGGTGGCACCCTATGAACTTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCa     <  1:1308647/150‑1 (MQ=255)
                                                                                                                                                cAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCAc    <  1:1279426/150‑1 (MQ=255)
                                                                                                                                                  aCTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCAcca  >  1:735402/1‑149 (MQ=255)
                                                                                                                                                    tCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtgg                                                        >  1:1722673/1‑94 (MQ=255)
                                                                                                                                                    tCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtgg                                                        <  2:1722673/94‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGCCACCG  >  NC_002947/2403989‑2404284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑