Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 213,354 T→A 6.0% L6287Q (CTG→CAG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947213,3540TA 6.0% 306.1 / 7.9 99L6287Q (CTG→CAG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (46/47);  new base A (2/4);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 105 total aligned reads displayed.

AGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGTG  >  NC_002947/213209‑213503
                                                                                                                                                 |                                                                                                                                                     
ggctcGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                                   >  2:244957/5‑150 (MQ=255)
aGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                                   <  1:2311090/150‑1 (MQ=255)
 gCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGCCCAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCtcgccg                                                                                                                                                  >  2:2302226/1‑145 (MQ=255)
  cACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGGTCACGACCAGGTCGt                                                                                                                                                 <  2:1604233/150‑1 (MQ=255)
    cGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGa                                                                                                                                               <  2:2138455/150‑1 (MQ=255)
     gTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGag                                                                                                                                              >  2:2124365/1‑149 (MQ=255)
      tCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGAcc                                                                                                                                             <  2:2446011/150‑1 (MQ=255)
        ggTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCt                                                                                                                                           >  2:741341/1‑150 (MQ=255)
          tGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGAGCAGGTCGTGAgccaag                                                                                                                                         <  2:1227475/150‑4 (MQ=255)
              cgacgaGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGCCCTCGGCGAAACCGACCGTCACACTTACTCGCACGTTGCACATCAACACCGCCTTGGCTCACGACCAGGTCGTGAgccaaggcgg                                                                                                                                     <  1:2124365/150‑8 (MQ=12)
              cgacgaGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGcaa                                                                                                                                     >  2:312668/1‑150 (MQ=255)
                   aGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATg                                                                                                                                <  2:1572753/150‑1 (MQ=255)
                   aGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATg                                                                                                                                <  1:244957/150‑1 (MQ=255)
                   aGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGATCGTGGTCTTGTCGTGACCCTGAGCAACAATg                                                                                                                                <  1:664502/150‑1 (MQ=18)
                      cGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGccc                                                                                                                             <  1:73249/150‑1 (MQ=255)
                         gTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAgg                                                                                                                          >  2:293588/1‑150 (MQ=255)
                             cccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcat                                                                                                                      >  2:2298182/1‑150 (MQ=255)
                              ccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc                                                                                                                     >  1:330945/1‑150 (MQ=255)
                              ccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc                                                                                                                     >  1:1723418/1‑150 (MQ=255)
                                aGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcat                                                                                                                   >  2:632400/1‑150 (MQ=255)
                                  gCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatca                                                                                                                 <  1:2298182/150‑1 (MQ=255)
                                        aaGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTg                                                                                                           <  1:979175/150‑1 (MQ=255)
                                         aGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatca                                                                                                                 <  2:1772529/143‑1 (MQ=255)
                                         aGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatca                                                                                                                 >  1:1772529/1‑143 (MQ=255)
                                               cTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGGTCACCGTTCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAAc                                                                                                    <  1:1122979/150‑1 (MQ=255)
                                                ttGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAAcc                                                                                                   >  1:1027533/1‑150 (MQ=255)
                                                 tGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACCATGCCCAGGTCATCATCAAGGCTGGCGAAACCa                                                                                                  <  1:907848/150‑1 (MQ=255)
                                                  ggTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTAACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                         >  1:1886383/1‑110 (MQ=255)
                                                  ggTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTAACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                         <  2:1886383/110‑1 (MQ=255)
                                                   gTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAgc                                                                                                <  1:1676644/150‑1 (MQ=255)
                                                    tCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg                                                                                                                           >  2:906943/1‑122 (MQ=255)
                                                    tCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg                                                                                                                           <  1:906943/122‑1 (MQ=255)
                                                         gTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                         <  1:1090370/103‑1 (MQ=255)
                                                         gTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                         >  2:1090370/1‑103 (MQ=255)
                                                         gTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCa                                                                                          <  2:1064841/150‑1 (MQ=255)
                                                          tCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCAt                                                                                         >  1:373145/1‑150 (MQ=255)
                                                              cATCACGGCCGACCGGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATacac                                                                                     <  2:1650690/150‑1 (MQ=255)
                                                                  aCGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCAc                                                                                 >  1:1704672/1‑150 (MQ=255)
                                                                   cGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACg                                                                                <  2:1704672/150‑1 (MQ=255)
                                                                     gCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGaa                                                                                                      <  2:720621/126‑1 (MQ=255)
                                                                     gCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGaa                                                                                                      >  1:720621/1‑126 (MQ=255)
                                                                        gACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTgcg                                                                           >  2:1998457/1‑150 (MQ=255)
                                                                          ccAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCa                                                                         >  1:1212767/1‑150 (MQ=255)
                                                                             gACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAgg                                                                      >  1:1815609/1‑150 (MQ=255)
                                                                               ccTCGGTGGCCGAGAACGTCAGGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCg                                                                    >  1:202748/1‑150 (MQ=255)
                                                                                 tCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt                                                                  >  2:1825101/1‑150 (MQ=255)
                                                                                   ggTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGa                                                                <  2:373145/150‑1 (MQ=255)
                                                                                      ggCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGt                                                             <  1:2223137/150‑1 (MQ=255)
                                                                                       gCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACTCTGCGCAAGGCGATGACGTc                                                            <  1:1065513/150‑1 (MQ=255)
                                                                                       gCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTc                                                            <  1:1825101/150‑1 (MQ=255)
                                                                                       gCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTc                                                            <  1:1998457/150‑1 (MQ=255)
                                                                                       gCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTc                                                            >  1:2182226/1‑150 (MQ=255)
                                                                                             aaCGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTTGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAAc                                                      <  1:293588/150‑1 (MQ=255)
                                                                                               ggTCAACACGACGTTAACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGACGATGACGTCTACACCGa                                                    <  2:819168/149‑1 (MQ=18)
                                                                                                gTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAg                                                                                                                           <  1:237617/78‑1 (MQ=255)
                                                                                                gTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCaa                                                                        >  2:287064/1‑129 (MQ=255)
                                                                                                gTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCaa                                                                        <  1:287064/129‑1 (MQ=255)
                                                                                                gTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAGGACGTCTACAACGAt                                                   >  1:1425720/1‑150 (MQ=255)
                                                                                                   aaGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCt                                                >  2:114556/1‑150 (MQ=255)
                                                                                                       cGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTAc                                                         >  2:1539453/1‑137 (MQ=255)
                                                                                                       cGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTAc                                                         <  1:1539453/137‑1 (MQ=255)
                                                                                                       cGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGcc                                            >  2:323218/1‑150 (MQ=255)
                                                                                                        gACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCa                                           <  1:305522/150‑1 (MQ=255)
                                                                                                        gACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCa                                           <  2:202748/150‑1 (MQ=255)
                                                                                                           gTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAATGCGATGACGTCTACAACGATGCTGGCCAGAt                                        <  1:312668/150‑1 (MQ=255)
                                                                                                           gTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGAt                                        >  1:634953/1‑150 (MQ=255)
                                                                                                             tCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                      <  2:2021606/150‑1 (MQ=255)
                                                                                                              cACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAg                                     >  1:2119191/1‑150 (MQ=255)
                                                                                                               aCCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGa                                                                                                                                               >  2:351343/1‑43 (MQ=255)
                                                                                                               aCCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGa                                                                                                                                               <  1:351343/43‑1 (MQ=255)
                                                                                                               aCCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGc                                    <  1:632400/150‑1 (MQ=255)
                                                                                                                  gTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTg                                 <  2:634953/150‑1 (MQ=255)
                                                                                                                   tGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTgg                                >  1:849303/1‑150 (MQ=255)
                                                                                                                   tGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTgg                                <  1:2143396/150‑1 (MQ=255)
                                                                                                                      acaTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCa                             >  1:612613/1‑150 (MQ=255)
                                                                                                                         tCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTa                          <  1:741341/150‑1 (MQ=255)
                                                                                                                            acacCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGGGCCATACCCCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAtt                       >  2:918651/1‑150 (MQ=255)
                                                                                                                             cacCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTc                      >  2:1403348/1‑150 (MQ=255)
                                                                                                                               ccGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                      >  2:837493/1‑132 (MQ=255)
                                                                                                                               ccGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCa                                      <  1:837493/132‑1 (MQ=255)
                                                                                                                                cGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGc                   >  1:1068078/1‑150 (MQ=255)
                                                                                                                                 gCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCa                  <  2:2119191/150‑1 (MQ=255)
                                                                                                                                   cTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCACCTACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGt                <  1:536744/150‑1 (MQ=255)
                                                                                                                                       gCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTgg                                                                                                          <  1:2060934/56‑1 (MQ=255)
                                                                                                                                       gCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTgg                                                                                                          >  2:2060934/1‑56 (MQ=255)
                                                                                                                                         tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc          >  1:310325/1‑150 (MQ=255)
                                                                                                                                         tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc          >  2:753180/1‑150 (MQ=255)
                                                                                                                                         tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc          >  1:1800297/1‑150 (MQ=255)
                                                                                                                                         tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc          >  1:1883525/1‑150 (MQ=255)
                                                                                                                                         tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc          >  1:983428/1‑150 (MQ=255)
                                                                                                                                         tCACGACCAGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc          >  1:107004/1‑150 (MQ=255)
                                                                                                                                           aCGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCa        <  2:310325/150‑1 (MQ=255)
                                                                                                                                             gACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACt      >  2:833572/1‑150 (MQ=255)
                                                                                                                                                cTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGt   <  2:2182226/150‑1 (MQ=255)
                                                                                                                                                cTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGt   <  1:820264/150‑1 (MQ=255)
                                                                                                                                                 tGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTg                                               >  1:2310762/1‑105 (MQ=255)
                                                                                                                                                 tGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTg                                               <  2:2310762/105‑1 (MQ=255)
                                                                                                                                                 tGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGTg  >  2:1154661/1‑150 (MQ=255)
                                                                                                                                                 tGGTCGTGACCCTGAGCAACAATGCACAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGTg  >  1:65047/1‑150 (MQ=255)
                                                                                                                                                 tGGACGTGACCCGGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGTg  >  2:2441646/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
AGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACTGGTG  >  NC_002947/213209‑213503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑