Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,137 T→G 7.4% intergenic (+33/+101) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,1370TG 7.4% 148.9 / 4.6 54intergenic (+33/+101)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base G (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

GACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTT  >  NC_002947/2649988‑2650282
                                                                                                                                                     |                                                                                                                                                 
gACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGt                                                                                                                                                   <  1:838076/150‑1 (MQ=255)
 aCATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTg                                                                                                                                                  >  2:2058650/1‑150 (MQ=255)
    tGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAg                                                                                                                                               <  2:1033361/150‑1 (MQ=255)
     ggTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAgg                                                                                                                                              >  1:1874823/1‑150 (MQ=255)
      gTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGc                                                                                                                                             <  2:374652/150‑1 (MQ=255)
        gCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCACTAAGGGCAGGCCt                                                                                                                                           <  2:453788/150‑1 (MQ=255)
        gCGTGACGTGGTGGCTGCAGAAGATGGTATGACGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCt                                                                                                                                           >  2:737774/1‑150 (MQ=255)
           tGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCtttt                                                                                                                                        >  1:847264/1‑150 (MQ=255)
                tggtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAGCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTAc                                                                                                                                   <  2:1638182/150‑1 (MQ=255)
                tggtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTAc                                                                                                                                   <  1:880768/150‑1 (MQ=255)
                        gCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTg                                                                                                                                                  >  2:465783/1‑127 (MQ=255)
                        gCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTg                                                                                                                                                  <  1:465783/127‑1 (MQ=255)
                         cAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCGTGTCGTACCTGCTGAcc                                                                                                                          >  2:1838934/1‑150 (MQ=255)
                           gaagaTGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACcgc                                                                                                                        <  1:737774/150‑1 (MQ=255)
                            aagaTGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACcgcg                                                                                                                       >  2:1191910/1‑150 (MQ=255)
                                       ccGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGttt                                                                                                            <  2:1874823/150‑1 (MQ=255)
                                            aGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                       <  1:1381648/150‑1 (MQ=255)
                                            aGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                       <  2:1022285/150‑1 (MQ=255)
                                            aGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                       <  1:2058650/150‑1 (MQ=255)
                                            aGAAATTGCCGTGCCCGTGGACAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAg                                                                                                       <  2:553323/150‑1 (MQ=255)
                                                            gTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGa                                                                                       >  2:2106838/1‑150 (MQ=255)
                                                                   aCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACaa                                                                                <  2:577670/150‑1 (MQ=255)
                                                                    ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACctgct                                                                                                                              <  1:616919/103‑1 (MQ=255)
                                                                    ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACctgct                                                                                                                              >  2:616919/1‑103 (MQ=255)
                                                                     cAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGcc                                                                                         <  2:2337348/139‑1 (MQ=255)
                                                                     cAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGcc                                                                                         >  1:2337348/1‑139 (MQ=255)
                                                                       gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTgg                                                                            <  1:584474/150‑1 (MQ=255)
                                                                             cGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGc                                                                      >  1:323002/1‑150 (MQ=255)
                                                                                 ccGATGGTGACGAGCATGCCGCCGGCACTGCAGTAAGGTAAGTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAAGAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                                  <  1:880436/150‑1 (MQ=255)
                                                                                     tGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGcttttttat                                                              <  1:1810362/150‑7 (MQ=255)
                                                                                      ggTGACGAGCATTCCGCCTGAACTGCAGTACGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGcttttttatt                                                             <  1:2180108/150‑5 (MQ=255)
                                                                                            gAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCgc                                                       >  2:2389295/1‑149 (MQ=255)
                                                                                                 ttCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGtgatga                                                  >  1:1216542/1‑150 (MQ=255)
                                                                                                  tCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGat                                                 >  2:1896155/1‑150 (MQ=255)
                                                                                                     gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAgg                                                                                                      >  1:2007419/1‑94 (MQ=255)
                                                                                                     gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAgg                                                                                                      <  2:2007419/94‑1 (MQ=255)
                                                                                                       tcGAACTGCAGTAAGGTAATTTCGGCGTCCCACGGACCGCTTTTAGTGTAGGCCTTTTCGTATCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGAttatta                                            <  2:390959/149‑1 (MQ=255)
                                                                                                         gAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACt                                          >  1:789575/1‑150 (MQ=255)
                                                                                                                aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGc                                                                      >  1:1642690/1‑115 (MQ=255)
                                                                                                                aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGc                                                                      <  2:1642690/115‑1 (MQ=255)
                                                                                                                aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttctt                                                                 >  2:619609/1‑120 (MQ=255)
                                                                                                                aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttctt                                                                 <  1:619609/120‑1 (MQ=255)
                                                                                                                 gTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctg                                  >  2:1994812/1‑150 (MQ=255)
                                                                                                                                     ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg              >  1:1870825/1‑150 (MQ=255)
                                                                                                                                     ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg              >  2:1291895/1‑150 (MQ=255)
                                                                                                                                     atgagCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg              >  1:1663636/5‑150 (MQ=255)
                                                                                                                                      aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCg                                                                        >  2:2243821/2‑91 (MQ=39)
                                                                                                                                      aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCg                                                                        <  1:2243821/90‑1 (MQ=39)
                                                                                                                                         gCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcg          >  2:158008/1‑150 (MQ=255)
                                                                                                                                           cTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcgcg        >  1:774630/1‑150 (MQ=255)
                                                                                                                                                cTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGt   >  2:1755578/1‑150 (MQ=255)
                                                                                                                                                cTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGt   >  2:1607652/1‑150 (MQ=255)
                                                                                                                                                cTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGt   >  1:340394/1‑150 (MQ=255)
                                                                                                                                                 ttAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGtt  <  2:774630/150‑1 (MQ=255)
                                                                                                                                                 ttAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCAACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGtt  >  1:2434768/1‑150 (MQ=255)
                                                                                                                                                     tGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATcc                                                         <  2:2272401/91‑1 (MQ=255)
                                                                                                                                                     tGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATcc                                                         >  1:2272401/1‑91 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTT  >  NC_002947/2649988‑2650282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑