Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,732,661 A→T 5.9% Q652L (CAG→CTG)  polB → DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,732,6610AT 5.9% 256.7 / 5.4 85Q652L (CAG→CTG) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (40/39);  new base T (2/3);  total (42/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCGCTGGCTGATTACCAGCGCAAC  >  NC_002947/2732512‑2732809
                                                                                                                                                     |                                                                                                                                                    
cTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGACTGGAGAGGGAGCGCACCGACTTGTGACCGCTGGCACGGCACCTCca                                                                                                                                                      >  2:390266/1‑150 (MQ=255)
 ttCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcag                                                                                                                                                     >  2:915736/1‑150 (MQ=255)
   ccTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcagca                                                                                                                                                   <  1:327851/150‑1 (MQ=255)
   ccTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCcagca                                                                                                                                                   <  2:1762390/150‑1 (MQ=255)
    cTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGCCGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCTCCGCTGGCCCGGCAGTTCcagcag                                                                                                                                                  <  2:471678/150‑1 (MQ=255)
     tGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTTGGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAgg                                                                                                                                                 >  1:391767/1‑150 (MQ=255)
        tGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAAc                                                                                                                                              <  1:2004630/150‑1 (MQ=255)
         gCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAAct                                                                                                                                             >  1:193770/1‑150 (MQ=255)
            caccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTa                                                                                                                                          >  1:2160693/1‑150 (MQ=255)
            caccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTa                                                                                                                                          >  2:1913293/1‑150 (MQ=255)
            caccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTa                                                                                                                                          >  1:1539442/1‑150 (MQ=255)
            caccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCCGGCCCGGCAGTTCCAGCAGGAACTCTa                                                                                                                                          >  2:2241850/1‑150 (MQ=255)
             accaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCTGCTGGAActgccg                                                                                                                                         <  1:1877878/150‑5 (MQ=255)
             accaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                                                                         <  2:1712670/150‑1 (MQ=255)
             accaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                                                                         <  2:1806306/150‑1 (MQ=255)
             accaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                                                                         <  1:2157661/150‑1 (MQ=255)
              ccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                                                                         >  2:2337666/1‑149 (MQ=255)
              ccaTCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTAc                                                                                                                                         <  1:2337666/149‑1 (MQ=255)
              ccaTCCCCGGCATCGACTTGGTCAGGAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCTGCTGGAActgccgg                                                                                                                                        <  2:1321006/150‑6 (MQ=255)
                 tgcgcgGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCCGGCCCGGCAGTTCCAGCAGGAACTCTACGGcc                                                                                                                                     <  1:2241850/148‑1 (MQ=255)
                   cgcgGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgt                                                                                                                                   <  1:1913293/150‑1 (MQ=255)
                   cgcgGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgt                                                                                                                                   <  1:986296/150‑1 (MQ=255)
                     cgGCACCGACGGGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgtgt                                                                                                                                 <  2:421387/150‑1 (MQ=255)
                        cACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTTCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGtt                                                                                                                              >  1:545253/1‑150 (MQ=255)
                         aCCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTc                                                                                                                             >  1:1087752/1‑150 (MQ=255)
                         aCCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTc                                                                                                                             <  1:1504482/150‑1 (MQ=255)
                          ccGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCGCCGCCCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTcc                                                                                                                            >  1:1333037/1‑150 (MQ=255)
                                 gggCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGa                                                                                                                     <  1:2118904/150‑1 (MQ=255)
                                     agcaAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGccc                                                                                                                 >  2:2255537/1‑150 (MQ=255)
                                     agcaAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGccc                                                                                                                 >  2:229773/1‑150 (MQ=255)
                                             gcgcTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGa                                                                                                         <  1:2069217/150‑1 (MQ=255)
                                             gcgcTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGa                                                                                                         <  2:947848/150‑1 (MQ=255)
                                             gcgcTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTACGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgtgt                                                                                                                                 >  2:754592/1‑126 (MQ=255)
                                             gcgcTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTACGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCgtgt                                                                                                                                 <  1:754592/126‑1 (MQ=255)
                                                    gCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCAAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTc                                                                                                  >  2:1487285/1‑150 (MQ=255)
                                                      cGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCcg                                                                                                <  2:2389822/150‑1 (MQ=255)
                                                         ccTGGTGCAGCGGGCCGGCGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCcgcgg                                                                                             >  1:295789/1‑149 (MQ=255)
                                                         ccTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGa                                                                                             <  2:929254/150‑1 (MQ=255)
                                                           tGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGt                                                                                           <  2:1087752/150‑1 (MQ=255)
                                                                  gCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACg                                                                                    >  2:950350/1‑150 (MQ=255)
                                                                              tGCTGAAGAAATGGTTTACAAGGGACTCGATGCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggg                                                                        >  1:565542/1‑149 (MQ=255)
                                                                                 tGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAg                                                                                                                    >  2:471491/1‑103 (MQ=255)
                                                                                 tGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAg                                                                                                                    <  1:471491/103‑1 (MQ=255)
                                                                                 tGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcggg                                                                     >  2:2069502/1‑150 (MQ=255)
                                                                                   aagaaATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAAAGTGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcgggcg                                                                   >  1:1354151/1‑150 (MQ=255)
                                                                                    agcaaTGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACGGGTCCCGGTTGGGCCTGCGGTTCCCGCAGGATCTCTACGGCCGTGTGGTCCGCAGCGGGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGa                                                                  <  2:565542/147‑1 (MQ=255)
                                                                                    agaaATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGa                                                                  <  2:545253/150‑1 (MQ=255)
                                                                                    agaaATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGa                                                                  <  2:1530193/150‑1 (MQ=255)
                                                                                     gaaATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAg                                                                 <  2:1333037/150‑1 (MQ=255)
                                                                                        aTGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAg                                                              <  1:1341956/150‑1 (MQ=255)
                                                                                          ggTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGa                                                            <  2:193770/150‑1 (MQ=255)
                                                                                            tttACAAGGGCCTGGCGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGCGTTCCGCAGCGAGCCCGACCGTGATTATGTCCGCGAGTATGTACGGCGCGCGTCGGCGGGGGAGCGGGACg                                                          >  2:1545966/1‑150 (MQ=255)
                                                                                            tttACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTcc                                                                                                                            >  2:2259391/1‑84 (MQ=255)
                                                                                            tttACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTcc                                                                                                                            <  1:2259391/84‑1 (MQ=255)
                                                                                               aCAAGGGCCTGGAGCCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAAc                                                       >  2:2180208/1‑150 (MQ=255)
                                                                                                 aaGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTACAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActg                                                     >  1:749415/1‑150 (MQ=255)
                                                                                                  aGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgc                                                    <  1:915736/150‑1 (MQ=255)
                                                                                                     gCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTgg                                                 >  2:1925695/1‑150 (MQ=255)
                                                                                                        accaGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGttt                                              >  2:470555/4‑150 (MQ=255)
                                                                                                         ggAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTa                                             <  2:1539442/150‑1 (MQ=255)
                                                                                                          gAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTACAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTAt                                            <  2:749415/150‑1 (MQ=255)
                                                                                                          caGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTAt                                            >  1:874801/2‑150 (MQ=255)
                                                                                                               ggTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTAcc                                                                                                             >  2:891807/1‑80 (MQ=255)
                                                                                                               ggTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTAcc                                                                                                             <  1:891807/80‑1 (MQ=255)
                                                                                                               ggTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCaa                                       <  2:2160693/150‑1 (MQ=255)
                                                                                                               ggTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCaa                                       <  2:1132614/150‑1 (MQ=255)
                                                                                                                     cACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcgggc                                                                    >  2:2105100/1‑115 (MQ=255)
                                                                                                                     cACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggcgggc                                                                    <  1:2105100/115‑1 (MQ=255)
                                                                                                                       ccGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGc                               <  1:2180208/150‑1 (MQ=255)
                                                                                                                       ccGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGc                               >  1:1230598/1‑150 (MQ=255)
                                                                                                                        cGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgctg                                                  <  2:1430074/130‑1 (MQ=255)
                                                                                                                        cGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgctg                                                  >  1:1430074/1‑130 (MQ=255)
                                                                                                                              gTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGccc                        >  2:1375239/1‑150 (MQ=255)
                                                                                                                               tCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCg                       >  2:1528238/1‑150 (MQ=255)
                                                                                                                               tCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCg                       >  2:647131/1‑150 (MQ=255)
                                                                                                                                 gccGCTGGCCCGGCAGTGCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCgct                     <  1:1975738/149‑1 (MQ=255)
                                                                                                                                     cTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCgctggct                 <  1:1925695/150‑1 (MQ=255)
                                                                                                                                         cccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCGCTGGCTGAtt             >  2:679749/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggc                                                                        <  1:2194727/90‑1 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTggc                                                                        >  2:2194727/1‑90 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgctg                                                  >  1:1895458/1‑112 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAActgctg                                                  <  2:1895458/112‑1 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCaa                                       <  1:1237209/123‑1 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCaa                                       >  2:1237209/1‑123 (MQ=255)
                                                                                                                                          ccGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCGCTGGCTGATTa            >  1:1195436/1‑150 (MQ=255)
                                                                                                                                           gtagaGTTCCTGCTGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCAc                                                                              <  1:2429068/79‑1 (MQ=255)
                                                                                                                                           gtagaGTTCCTGCTGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCAc                                                                              >  2:2429068/5‑83 (MQ=255)
                                                                                                                                            tagaGTTCCTGCTGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCGCTGGCTGATTAcc          >  2:2125390/4‑150 (MQ=255)
                                                                                                                                                 ttCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTg                                >  2:1585120/1‑123 (MQ=255)
                                                                                                                                                 ttCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTg                                <  1:1585120/123‑1 (MQ=255)
                                                                                                                                                    cagcagGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCGCTGGCTGATTACCAGCGCAAc  >  1:1256238/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTCCTGATGCCCACCATCCGCGGCACCGACGAGGGCAGCAAGAAGCGCTATGCCGGCCTGGTGCAGCGGGCCGACGGTGCTGAAGAAATGGTTTACAAGGGCCTGGAGTCGGTGCGCACCGACTGGTCACCGCTGGCCCGGCAGTTCCAGCAGGAACTCTACGGCCGTGTGTTCCGCAGCGAGCCCTACCGTGATTATGTCCGCGAGTATGTACGGCGCACGTTGGCGGGCGAGCAGGACGAACTGCTGGTTTATCGCAAGCGCCTGCGTCGCCCGCTGGCTGATTACCAGCGCAAC  >  NC_002947/2732512‑2732809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑