BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	226,226	T→A	9.5%	L151Q (CTG→CAG)	PP_0174 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	226,226	0	T	A	9.5%	314.7 / 19.7	108	L151Q (CTG→CAG)	PP_0174	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (49/47); new base A (3/7); total (53/55)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 114 total aligned reads displayed.

CGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAC  >  NC_002947/226080‑226374

cGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCa                                                                                                                                                   <  1:499172/150‑1 (MQ=255)

cGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAAGGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCa                                                                                                                                                   <  2:1000924/150‑1 (MQ=255)

        tGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgccg                                                                                                                                           >  1:113371/1‑150 (MQ=255)

        atttGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcgg                                                                                                                                           <  1:1554993/148‑3 (MQ=255)

         gTTGCGCATGGCGGCCCTGCGGGGCCCTGGCGCTATCGGATTGTATGTGTGCCGGCTGCACTGCTTCGGCAAAGGCATCGACCCTTCCTCTGTGCAGGCGGCGCAGTGTTTCCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcggt                                                                                                                                          <  2:2091454/150‑4 (MQ=255)

         gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTACGGCCATGACAGCGACCCTTCCTAGGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcggt                                                                                                                                          <  1:885194/150‑4 (MQ=255)

             cgcATGGCGGCCCTGCGGGGCAAAGCTGCTGCCTGGGTCAACCTTGGCCAGCAGCACCACTTCGGCCAAGGCGTCGACCCTTCCTATGTGCAGGCGGCGGAGGGGTACCGGCAGGCCCTCGACCCCCGCGCGCCACATgccgcgggagag                                                                                                                                      >  2:1554993/1‑143 (MQ=255)

              gcATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACGACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCAc                                                                                                                                     >  2:1593931/1‑150 (MQ=255)

                 tGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTgg                                                                                                                                  <  1:226634/150‑1 (MQ=255)

                     ggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGa                                                                                                                              >  1:2198670/1‑150 (MQ=255)

                       cccTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGTGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGAcc                                                                                                                            >  2:1743241/1‑150 (MQ=255)

                       cccTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGAcc                                                                                                                            >  1:1708864/1‑150 (MQ=255)

                               ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    <  2:1708864/150‑1 (MQ=255)

                               ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    <  1:963435/150‑1 (MQ=255)

                               ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    >  2:1364438/1‑150 (MQ=255)

                               ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    >  2:204018/1‑150 (MQ=255)

                               ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    <  1:606707/150‑1 (MQ=255)

                               ggCAATGCTGCTGCCTGGTTCAACCTTGCCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCATCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                    >  2:1252632/1‑150 (MQ=255)

                                 cAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgagga                                                                                                                  >  2:538745/1‑150 (MQ=255)

                                    tgctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggagga                                                                                                               <  2:2283775/150‑1 (MQ=255)

                                        gctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCAtgct                                                                                                                                                      <  1:880714/107‑1 (MQ=255)

                                        gctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCAtgct                                                                                                                                                      >  2:880714/1‑107 (MQ=255)

                                         ctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGgtgt                                                                                                          <  2:1164905/150‑1 (MQ=255)

                                          tgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGtgtg                                                                                                         <  1:538745/150‑1 (MQ=255)

                                           gcCTGGTTCAACCTTGGCCAGCAGCCCCAACCCAGCACCTGTTGCTAGGCTGCCTATGTGCAGGCGGCGGAGCTTTTCCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGc                                                                                                        <  1:532890/150‑1 (MQ=255)

                                           gcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGc                                                                                                        <  1:1942170/150‑1 (MQ=255)

                                               ggTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACg                                                                                                    <  1:1098780/150‑1 (MQ=255)

                                                gTTCAACCTTGGCCATCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgcc                                                                                                                                               <  2:918671/106‑1 (MQ=255)

                                                gTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACgg                                                                                                   >  1:8016/1‑150 (MQ=255)

                                                gTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACgg                                                                                                   >  2:487134/1‑150 (MQ=255)

                                                gTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgcc                                                                                                                                               >  1:918671/1‑106 (MQ=255)

                                                 ttCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGgt                                                                                                            >  1:1005986/1‑140 (MQ=255)

                                                 ttCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGt                                                                                                  <  1:845596/150‑1 (MQ=255)

                                                      ccTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                             >  1:2314799/1‑150 (MQ=255)

                                                      ccTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                             >  2:2061406/1‑150 (MQ=255)

                                                      ccTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                             >  1:2239618/1‑150 (MQ=255)

                                                      ccTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                             >  1:208477/1‑150 (MQ=255)

                                                       cTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTg                                                                                            <  1:2061406/150‑1 (MQ=255)

                                                        ttGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTgg                                                                                           >  1:1745280/1‑150 (MQ=255)

                                                            ccAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATg                                                                                       >  1:1413519/1‑150 (MQ=255)

                                                               gcagcaCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCa                                                                                    >  2:1456260/1‑150 (MQ=255)

                                                                  gcaCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGt                                                                                 <  2:1180780/150‑1 (MQ=255)

                                                                   caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGtt                                                                                >  1:55847/1‑150 (MQ=255)

                                                                       actTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGAcc                                                                            >  1:807376/1‑150 (MQ=255)

                                                                        ctTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGCCCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCt                                                                           <  2:851858/150‑1 (MQ=255)

                                                                         ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                          >  1:842399/1‑150 (MQ=255)

                                                                           cGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGTGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGt                                                                        >  2:793547/1‑150 (MQ=255)

                                                                           cGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGt                                                                        <  1:487134/150‑1 (MQ=255)

                                                                            ggCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTg                                                                       >  1:782837/1‑150 (MQ=255)

                                                                            ggCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcgg                                                                                                                                           >  2:2415270/1‑80 (MQ=255)

                                                                            ggCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcgg                                                                                                                                           <  1:2415270/82‑3 (MQ=255)

                                                                                 aGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGc                                                                  <  1:667894/150‑1 (MQ=255)

                                                                                   gCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCt                                                                >  1:302782/1‑150 (MQ=255)

                                                                                   gCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCt                                                                >  1:1906254/1‑150 (MQ=255)

                                                                                      tCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATc                                                             >  2:1947671/1‑150 (MQ=255)

                                                                                       cGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCa                                                            <  2:506686/150‑1 (MQ=255)

                                                                                         aCCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCCGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAACGGCAGGTTGACCTGGTGCAAGCCTATCAGt                                                          >  1:1200700/1‑150 (MQ=255)

                                                                                          cccTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCATGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTg                                                         <  1:1440877/150‑1 (MQ=255)

                                                                                            cTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCTGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGa                                                                              >  2:965612/1‑127 (MQ=255)

                                                                                            cTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCTGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGa                                                                              <  1:965612/127‑1 (MQ=255)

                                                                                                cTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTTGTGCAAGCCTATCAGTGGTTCtt                                                   >  2:1160752/1‑150 (MQ=255)

                                                                                                 tATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTg                                                  <  2:842399/150‑1 (MQ=255)

                                                                                                     tgCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTg                                              >  2:316994/1‑150 (MQ=255)

                                                                                                     tgCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTg                                              >  2:563013/1‑150 (MQ=255)

                                                                                                     tgCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAACGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTg                                              <  2:1200700/150‑1 (MQ=255)

                                                                                                      gCAGGCGGCGGAGTGTTCCCGGCAGGCCTTCGACCGCGGCATTCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCTTATCAGTGGTTCTTGCGTgg                                             <  1:1036912/150‑1 (MQ=255)

                                                                                                       cAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGgc                                            <  2:55847/150‑1 (MQ=255)

                                                                                                          gcggcgGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGcc                                         >  2:1571728/1‑150 (MQ=255)

                                                                                                             gcggAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGaa                                      >  2:353769/1‑150 (MQ=255)

                                                                                                               ggAGTGTTACCTGCAGGCCTTCAACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTGTGAGGAGGAGGTGTGCGAGGGTCCCCGACAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTATTGCATGGCGCCGAACa                                    >  2:323352/1‑150 (MQ=255)

                                                                                                                 aGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAgg                                  <  1:204018/150‑1 (MQ=255)

                                                                                                                   tgtTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGc                                <  2:2239618/150‑1 (MQ=255)

                                                                                                                     ttACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                               <  1:428508/133‑1 (MQ=255)

                                                                                                                     ttACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                               >  2:428508/1‑133 (MQ=255)

                                                                                                                     ttACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGa                              >  1:2436368/1‑150 (MQ=255)

                                                                                                                        ccGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGgc                           <  1:1456260/150‑1 (MQ=255)

                                                                                                                             aGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                          <  1:636326/98‑1 (MQ=255)

                                                                                                                             aGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTTGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGtt                      <  1:1160752/150‑1 (MQ=255)

                                                                                                                                ccTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTa                                                               <  1:978121/106‑1 (MQ=255)

                                                                                                                                ccTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                   >  1:97284/1‑150 (MQ=255)

                                                                                                                                 cTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                               <  1:824012/121‑1 (MQ=255)

                                                                                                                                 cTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                               >  2:824012/1‑121 (MQ=255)

                                                                                                                                  ttCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTgg                                                        <  1:107539/111‑1 (MQ=255)

                                                                                                                                  ttCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTgg                                                        >  2:107539/1‑111 (MQ=255)

                                                                                                                                  ttCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGt                 >  1:1156708/1‑150 (MQ=255)

                                                                                                                                   tCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGtt                <  1:793547/150‑1 (MQ=255)

                                                                                                                                    cggcggcgGCATGCAGCATGCCGCGGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGttt               <  1:356683/145‑1 (MQ=255)

                                                                                                                                     gACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGTGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGtttt              <  1:1364438/150‑1 (MQ=255)

                                                                                                                                     gACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGtttt              <  2:2314799/150‑1 (MQ=255)

                                                                                                                                       ccGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGa            >  1:1463232/1‑150 (MQ=255)

                                                                                                                                         gcgGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGaaa          <  1:563013/150‑1 (MQ=255)

                                                                                                                                         gcgGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGaaa          >  2:166937/1‑150 (MQ=255)

                                                                                                                                          cgGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAAt         >  1:2009025/1‑150 (MQ=255)

                                                                                                                                           ggCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATg        <  2:2009025/150‑1 (MQ=255)

                                                                                                                                            gCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATgg       <  2:1833789/150‑1 (MQ=255)

                                                                                                                                             cATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATggg      <  1:1427462/150‑1 (MQ=255)

                                                                                                                                               tgctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCt    >  2:520837/1‑150 (MQ=255)

                                                                                                                                                 ctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAc  >  1:208949/1‑150 (MQ=255)

                                                                                                                                                  tgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGt                                                                        <  2:2263075/79‑1 (MQ=255)

                                                                                                                                                  tgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGt                                                                        >  1:2263075/1‑79 (MQ=255)

CGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAC  >  NC_002947/226080‑226374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`