BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,890,482	T→A	7.1%	P57P (CCA→CCT) ‡	PP_2544 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,890,482	0	T	A	7.1%	326.3 / 12.5	110	P57P (CCA→CCT) ‡	PP_2544	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (53/49); new base A (3/5); total (56/54)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

Only 100 of 111 total aligned reads displayed.

CCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGTGCG  >  NC_002947/2890333‑2890628

ccATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGAACAGGTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCGtt                                                                                                                                                         >  1:626820/1‑150 (MQ=255)

  aTGGCCTTCATGCCTGACTACCAGATCCTGGGCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                       <  2:2378612/150‑1 (MQ=255)

    ggCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgggg                                                                                                                                                     >  1:1627447/1‑150 (MQ=255)

       cTTCATGCCTGACTACCAGATCCTGGACATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGAAACCGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                                >  2:1209960/1‑150 (MQ=255)

        ttCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Accc                                                                                                                                               <  2:1558184/150‑1 (MQ=255)

         tCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCg                                                                                                                                              <  1:2444844/150‑1 (MQ=255)

            tGCCAGACTACCAGATCCTGGTCATTTCCAACGGTATCGCAGCGGCGGTCGGGATGGCGGTGGGTGACGTGGACCCGATCCGCTCTGGTAGCAAGCAGCACTAAGCATCACTAGCCTCTGGAAACGGCCGGCTCACTTGGGG‑‑ACCCCGcc                                                                                                                                           >  2:1121266/1‑150 (MQ=255)

                   cTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTGGTGGTGGCGATGGGTGTGGTGCATCAAAGGCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcgc                                                                                                                                    <  1:1614156/150‑1 (MQ=255)

                   cTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcgc                                                                                                                                    <  2:2350613/150‑1 (MQ=255)

                    tACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                       <  1:2080427/132‑1 (MQ=255)

                    tACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                       >  2:2080427/1‑132 (MQ=255)

                      ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                                 >  1:1271535/1‑150 (MQ=255)

                      ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                                 >  1:341646/1‑150 (MQ=255)

                      ccAGATCCTGGTCATTTCCACCGGTATCACTGCGGCGGTCGTGGTGGCGATGGGTGGGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCGCTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                                 >  2:1255026/1‑150 (MQ=255)

                         gATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTATGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgttgtt                                                                                                                              >  1:2188895/1‑150 (MQ=255)

                             cTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTTGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTTCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc                                                                                                                          <  1:1196284/150‑1 (MQ=255)

                             cTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc                                                                                                                          >  1:312927/1‑150 (MQ=255)

                              tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGt                                                                                                                                         <  1:374112/134‑1 (MQ=39)

                              tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGt                                                                                                                                         >  2:374112/1‑134 (MQ=39)

                              tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc‑c                                                                                                                        >  1:2192782/1‑150 (MQ=255)

                               ggTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑Ct                                                                                                                       <  2:165956/150‑1 (MQ=255)

                                   aTTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGc                                                                                                                   >  1:1567108/1‑150 (MQ=255)

                                      tCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCCCGTTGTTGGGC‑CTGGGCtt‑t                                                                                                               <  1:1209960/150‑1 (MQ=255)

                                        cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGAG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCtt‑ttt                                                                                                             >  2:761134/1‑150 (MQ=255)

                                         aCCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                                <  2:1979456/116‑1 (MQ=255)

                                            ggTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                         <  1:237793/150‑1 (MQ=255)

                                             gTATCGCTGCGGCGGTCGTGGGGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                        <  2:1566545/150‑1 (MQ=255)

                                              tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        <  1:1410631/119‑1 (MQ=38)

                                              tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        <  1:1412770/119‑1 (MQ=38)

                                              tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        >  2:1410631/1‑119 (MQ=38)

                                                  gCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAt                                                                                                   >  2:199303/1‑150 (MQ=255)

                                                  gCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAt                                                                                                   >  1:1791630/1‑150 (MQ=255)

                                                    tGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAtgt                                                                                                 <  1:1811350/150‑1 (MQ=255)

                                                       ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcca                                                                                                                                    >  1:2445157/1‑112 (MQ=255)

                                                       ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcca                                                                                                                                    <  2:2445157/114‑3 (MQ=255)

                                                       ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CCGGGCTT‑TTTCTGCGGGCATGTGGa                                                                                              <  2:1627447/150‑1 (MQ=255)

                                                          ggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAAc                                                                                           >  1:1034618/1‑150 (MQ=255)

                                                            tCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGc                                                                                         <  2:2178240/150‑1 (MQ=255)

                                                               tggtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGTATCGGCCGGCCCCCGTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTgg                                                                                      <  2:314232/150‑1 (MQ=255)

                                                               tggtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTgg                                                                                      <  1:2278613/150‑1 (MQ=255)

                                                                 gtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGAAAACGCTGGtt                                                                                    >  2:163363/1‑150 (MQ=255)

                                                                    gCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTcct                                                                                 >  2:2389084/1‑148 (MQ=255)

                                                                         gggTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTTGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACg                                                                            <  1:161264/150‑1 (MQ=255)

                                                                                gtgCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AAcc                                                                    >  2:1606353/1‑150 (MQ=255)

                                                                                  gCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGCGCCG‑‑CGGCACCATTCGCGCCACCCTCCCGTTTCGCGTGGGTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGa                                                                  <  1:2389084/150‑1 (MQ=255)

                                                                                   cACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAg                                                                 >  1:85650/1‑150 (MQ=255)

                                                                                    aCCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGc                                                                >  1:765790/1‑150 (MQ=255)

                                                                                     ccAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               >  1:1186845/1‑150 (MQ=255)

                                                                                     ccAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTG‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               >  2:2277511/1‑150 (MQ=255)

                                                                                      cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                        >  1:1373959/1‑109 (MQ=255)

                                                                                      cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                        <  2:1373959/109‑1 (MQ=255)

                                                                                      cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAg                                                              >  1:749394/1‑150 (MQ=255)

                                                                                       aGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACAGCCGGCTACCCAGAGG‑‑ACCCCCCCGTACCCCGCTGCCCGGT‑CTGCGCTTCGTTCTTCTGTGTTG‑GTAATCTCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             <  2:2103063/150‑1 (MQ=255)

                                                                                        gATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAggg                                                            >  1:1509762/1‑150 (MQ=255)

                                                                                             gCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             <  2:104690/144‑1 (MQ=255)

                                                                                             gCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             >  1:104690/1‑144 (MQ=255)

                                                                                                ctGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCa                                                    <  1:1255026/150‑1 (MQ=255)

                                                                                                ctGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGAG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCa                                                    <  1:761134/150‑1 (MQ=255)

                                                                                                   gTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGAGG‑AAGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATAAACGGTG‑AACCGCGCAGGGCTGCCGCAGCg                                                 >  1:747394/1‑150 (MQ=255)

                                                                                                    tAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGC‑T‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGAt                                               >  1:628643/1‑150 (MQ=255)

                                                                                                       ctagcagcGCTAGGCGTCGCTCGCCTCTGGAACCGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                             <  2:692119/148‑1 (MQ=255)

                                                                                                       caagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                             <  1:305028/150‑1 (MQ=255)

                                                                                                       caagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGAAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                             <  1:163363/150‑1 (MQ=255)

                                                                                                         agcagcACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAgg                                           <  2:1034618/150‑1 (MQ=255)

                                                                                                         agcagcACTAAGCGTCACTCGCCTCTGCAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAgg                                           <  2:1271535/150‑1 (MQ=255)

                                                                                                          gcagcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAggg                                          >  1:1478211/1‑150 (MQ=255)

                                                                                                           cagcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGc                                         <  1:1129320/150‑1 (MQ=255)

                                                                                                            agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             <  1:604287/129‑1 (MQ=255)

                                                                                                            agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             >  2:604287/1‑129 (MQ=255)

                                                                                                            agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGcc                                        >  1:113824/1‑150 (MQ=255)

                                                                                                             gcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCa                                       >  2:173613/1‑150 (MQ=255)

                                                                                                             gcaCTAAGCGTCACTCGACTCTGGAAACGGCCGGCTCCCTTGGGC‑‑AAACCGCCGTTTC‑AATTTTCGCTCGTTGCTCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCa                                       <  1:1224148/150‑1 (MQ=255)

                                                                                                                 tAAGCGTGACTCGCCTCTGTAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGAt                                   <  2:1996129/150‑1 (MQ=255)

                                                                                                                 tAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGAt                                   >  2:2048820/1‑150 (MQ=255)

                                                                                                                    gCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGANGGc                                <  2:1509762/150‑1 (MQ=255)

                                                                                                                      gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTg                                                                                       >  2:431772/1‑94 (MQ=255)

                                                                                                                      gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTg                                                                                       <  1:431772/94‑1 (MQ=255)

                                                                                                                      gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                              <  2:1567108/150‑1 (MQ=255)

                                                                                                                      gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                              >  1:1342160/1‑150 (MQ=255)

                                                                                                                      gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATCTGGAAACGCTGGTGAATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                              >  2:1301332/1‑150 (MQ=255)

                                                                                                                        cACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAgg                            <  2:158370/150‑1 (MQ=255)

                                                                                                                        cACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAgg                            <  1:1028111/150‑1 (MQ=255)

                                                                                                                           tCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGGTGGGC‑CTGGGCTG‑TTTCTGCCGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGTATGGCGAGGGCg                         >  2:1326719/1‑150 (MQ=255)

                                                                                                                             gCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGAt                       <  1:395678/150‑1 (MQ=255)

                                                                                                                                tctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGC‑T‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAg                   <  2:628643/150‑1 (MQ=255)

                                                                                                                                 ctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAg                   >  2:2255262/1‑150 (MQ=255)

                                                                                                                                 ctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGCCCAGGATGGCGAGGGCGATGTAg                   <  2:1186845/150‑1 (MQ=255)

                                                                                                                                  tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               <  2:821638/105‑1 (MQ=255)

                                                                                                                                  tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               >  1:821638/1‑105 (MQ=255)

                                                                                                                                  tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGACGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                         >  2:1192076/1‑64 (MQ=255)

                                                                                                                                  tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGACGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                         <  1:1192076/64‑1 (MQ=255)

                                                                                                                                   ggAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGt                 <  1:173613/150‑1 (MQ=255)

                                                                                                                                    gAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTc                >  2:1999701/1‑150 (MQ=255)

                                                                                                                                       aCGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTc             >  2:301122/1‑150 (MQ=255)

                                                                                                                                          gccggcTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTT‑TTTAATGATTTAACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACg          >  1:1970954/1‑150 (MQ=255)

                                                                                                                                               cTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGt     <  2:747394/150‑1 (MQ=255)

                                                                                                                                                 cccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGTgc   <  2:2192782/150‑1 (MQ=255)

                                                                                                                                                  ccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAggg                           >  2:1747173/1‑125 (MQ=255)

                                                                                                                                                  ccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAggg                           <  1:1747173/125‑1 (MQ=255)

                                                                                                                                                  ccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTATTCACGGGCGTgcg  >  1:2404232/1‑150 (MQ=255)

CCATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGTGCG  >  NC_002947/2890333‑2890628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`