BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,890,483	G→A	6.8%	P57L (CCA→CTA) ‡	PP_2544 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,890,483	0	G	A	6.8%	297.0 / 9.2	105	P57L (CCA→CTA) ‡	PP_2544	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (51/45); new base A (3/4); total (54/51)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 110 total aligned reads displayed.

CATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGTGCG  >  NC_002947/2890334‑2890628

cATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTg                                                                                                                                                        <  1:630158/150‑1 (MQ=255)

 aTGGCCTTCATGCCTGACTACCAGATCCTGGGCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                       <  2:2378612/150‑1 (MQ=255)

   ggCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgggg                                                                                                                                                     >  1:1627447/1‑150 (MQ=255)

      cTTCATGCCTGACTACCAGATCCTGGACATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGAAACCGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                                >  2:1209960/1‑150 (MQ=255)

       ttCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Accc                                                                                                                                               <  2:1558184/150‑1 (MQ=255)

        tCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCg                                                                                                                                              <  1:2444844/150‑1 (MQ=255)

           tGCCAGACTACCAGATCCTGGTCATTTCCAACGGTATCGCAGCGGCGGTCGGGATGGCGGTGGGTGACGTGGACCCGATCCGCTCTGGTAGCAAGCAGCACTAAGCATCACTAGCCTCTGGAAACGGCCGGCTCACTTGGGG‑‑ACCCCGcc                                                                                                                                           >  2:1121266/1‑150 (MQ=255)

                gACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTc                                                                                                                                      >  2:305028/1‑150 (MQ=255)

                  cTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTGGTGGTGGCGATGGGTGTGGTGCATCAAAGGCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcgc                                                                                                                                    <  1:1614156/150‑1 (MQ=255)

                  cTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcgc                                                                                                                                    <  2:2350613/150‑1 (MQ=255)

                   tACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTgg                                                                                                                                                       <  1:2080427/132‑1 (MQ=255)

                    aCCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGGCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTGACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGTTTccaga                                                                                                                                  <  2:2166902/150‑5 (MQ=38)

                     ccAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                                 >  1:1271535/1‑150 (MQ=255)

                     ccAGATCCTGGTCATTTCCACCGGTATCACTGCGGCGGTCGTGGTGGCGATGGGTGGGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCGCTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCgtt                                                                                                                                 >  2:1255026/1‑150 (MQ=255)

                            cTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTTGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTTCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc                                                                                                                          <  1:1196284/150‑1 (MQ=255)

                            cTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc                                                                                                                          >  1:312927/1‑150 (MQ=255)

                             tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGt                                                                                                                                         >  2:374112/1‑134 (MQ=39)

                             tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGt                                                                                                                                         <  1:374112/134‑1 (MQ=39)

                             tGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGc‑c                                                                                                                        >  1:2192782/1‑150 (MQ=255)

                              ggTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑Ct                                                                                                                       <  2:165956/150‑1 (MQ=255)

                               gTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTg                                                                                                                      >  2:1681300/1‑150 (MQ=255)

                                  aTTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGc                                                                                                                   >  1:1567108/1‑150 (MQ=255)

                                     tCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCCCGTTGTTGGGC‑CTGGGCtt‑t                                                                                                               <  1:1209960/150‑1 (MQ=255)

                                       cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTCGCACGCCGCACTAAGCGTCACTCGCCTCTGCAAACGGCCGGCTCCGTTGGGG‑‑ACCCGGCCGTTTAGCGTGGTTAGGG‑CGGGTCgt‑ttt                                                                                                             >  2:1129320/1‑150 (MQ=255)

                                       cACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGAG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCtt‑ttt                                                                                                             >  2:761134/1‑150 (MQ=255)

                                        aCCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                                <  2:1979456/116‑1 (MQ=255)

                                        aCCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑Acc                                                                                                                                                >  1:1979456/1‑116 (MQ=255)

                                           ggTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                         <  1:237793/150‑1 (MQ=255)

                                            gTATCGCTGCGGCGGTCGTGGGGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                        <  2:1566545/150‑1 (MQ=255)

                                             tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        >  2:1410631/1‑119 (MQ=38)

                                             tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        >  2:1412770/1‑119 (MQ=38)

                                             tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        <  1:1412770/119‑1 (MQ=38)

                                             tATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGGTCCCCAAGGG‑‑AGCCGGCCGtt                                                                                                                                        <  1:1410631/119‑1 (MQ=38)

                                                 gCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAt                                                                                                   >  1:1791630/1‑150 (MQ=255)

                                                 gCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAt                                                                                                   >  2:199303/1‑150 (MQ=255)

                                                   tGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAtgt                                                                                                 <  1:1811350/150‑1 (MQ=255)

                                                   tGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCAtgt                                                                                                 <  1:300782/150‑1 (MQ=255)

                                                      ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcca                                                                                                                                    <  2:2445157/114‑3 (MQ=255)

                                                      ggcggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTcca                                                                                                                                    >  1:2445157/1‑112 (MQ=255)

                                                         ggTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAAc                                                                                           >  1:1034618/1‑150 (MQ=255)

                                                           tCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGc                                                                                         <  2:2178240/150‑1 (MQ=255)

                                                              tggtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGTATCGGCCGGCCCCCGTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTgg                                                                                      <  2:314232/150‑1 (MQ=255)

                                                              tggtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTgg                                                                                      <  1:2278613/150‑1 (MQ=255)

                                                                gtggCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGAAAACGCTGGtt                                                                                    >  2:163363/1‑150 (MQ=255)

                                                                   gCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTcct                                                                                 >  2:2389084/1‑148 (MQ=255)

                                                                               gtgCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AAcc                                                                    >  2:1606353/1‑150 (MQ=255)

                                                                                 gCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGCGCCG‑‑CGGCACCATTCGCGCCACCCTCCCGTTTCGCGTGGGTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGa                                                                  <  1:2389084/150‑1 (MQ=255)

                                                                                  cACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAg                                                                 >  1:85650/1‑150 (MQ=255)

                                                                                   aCCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGc                                                                >  1:765790/1‑150 (MQ=255)

                                                                                    ccAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               >  1:1186845/1‑150 (MQ=255)

                                                                                    ccAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTG‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               >  2:2277511/1‑150 (MQ=255)

                                                                                     cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                        >  1:1373959/1‑109 (MQ=255)

                                                                                     cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCg                                                                                                        <  2:1373959/109‑1 (MQ=255)

                                                                                     cAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAg                                                              >  1:749394/1‑150 (MQ=255)

                                                                                      aGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACAGCCGGCTACCCAGAGG‑‑ACCCCCCCGTACCCCGCTGCCCGGT‑CTGCGCTTCGTTCTTCTGTGTTG‑GTAATCTCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             <  2:2103063/150‑1 (MQ=255)

                                                                                       gATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAggg                                                            >  1:1509762/1‑150 (MQ=255)

                                                                                            gCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             <  2:104690/144‑1 (MQ=255)

                                                                                            gCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             >  1:104690/1‑144 (MQ=255)

                                                                                               ctGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCa                                                    <  1:1255026/150‑1 (MQ=255)

                                                                                               ctGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGAG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCa                                                    <  1:761134/150‑1 (MQ=255)

                                                                                                  gTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGAGG‑AAGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATAAACGGTG‑AACCGCGCAGGGCTGCCGCAGCg                                                 >  1:747394/1‑150 (MQ=255)

                                                                                                   tAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGC‑T‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGAt                                               >  1:628643/1‑150 (MQ=255)

                                                                                                      ctagcagcGCTAGGCGTCGCTCGCCTCTGGAACCGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                             <  2:692119/148‑1 (MQ=255)

                                                                                                      caagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                             <  1:305028/150‑1 (MQ=255)

                                                                                                      caagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGAAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCa                                             <  1:163363/150‑1 (MQ=255)

                                                                                                       aagcaGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGA‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAg                                            >  1:158370/1‑150 (MQ=255)

                                                                                                        agcagcACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAgg                                           <  2:1034618/150‑1 (MQ=255)

                                                                                                        agcagcACTAAGCGTCACTCGCCTCTGCAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAgg                                           <  2:1271535/150‑1 (MQ=255)

                                                                                                         gcagcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAggg                                          >  1:1478211/1‑150 (MQ=255)

                                                                                                          cagcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGc                                         <  1:1129320/150‑1 (MQ=255)

                                                                                                           agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             <  1:604287/129‑1 (MQ=255)

                                                                                                           agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAgg                                                             >  2:604287/1‑129 (MQ=255)

                                                                                                           agcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGcc                                        >  1:113824/1‑150 (MQ=255)

                                                                                                            gcaCTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCa                                       >  2:173613/1‑150 (MQ=255)

                                                                                                            gcaCTAAGCGTCACTCGACTCTGGAAACGGCCGGCTCCCTTGGGC‑‑AAACCGCCGTTTC‑AATTTTCGCTCGTTGCTCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCa                                       <  1:1224148/150‑1 (MQ=255)

                                                                                                                tAAGCGTGACTCGCCTCTGTAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGAt                                   <  2:1996129/150‑1 (MQ=255)

                                                                                                                tAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGAt                                   >  2:2048820/1‑150 (MQ=255)

                                                                                                                   gCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGANGGc                                <  2:1509762/150‑1 (MQ=255)

                                                                                                                     gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTg                                                                                       <  1:431772/94‑1 (MQ=255)

                                                                                                                     gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTg                                                                                       >  2:431772/1‑94 (MQ=255)

                                                                                                                     gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                              >  1:1342160/1‑150 (MQ=255)

                                                                                                                     gTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGa                              <  2:1567108/150‑1 (MQ=255)

                                                                                                                       cACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAgg                            <  1:1028111/150‑1 (MQ=255)

                                                                                                                       cACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAgg                            <  2:158370/150‑1 (MQ=255)

                                                                                                                          tCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGGTGGGC‑CTGGGCTG‑TTTCTGCCGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGTATGGCGAGGGCg                         >  2:1326719/1‑150 (MQ=255)

                                                                                                                            gCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGAt                       <  1:395678/150‑1 (MQ=255)

                                                                                                                               tctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGC‑T‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAg                   <  2:628643/150‑1 (MQ=255)

                                                                                                                                ctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAg                   >  2:2255262/1‑150 (MQ=255)

                                                                                                                                ctGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGCCCAGGATGGCGAGGGCGATGTAg                   <  2:1186845/150‑1 (MQ=255)

                                                                                                                                 tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCa                                                               <  2:821638/105‑1 (MQ=255)

                                                                                                                                 tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGACGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                         <  1:1192076/64‑1 (MQ=255)

                                                                                                                                 tGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGACGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGc                                                                                                         >  2:1192076/1‑64 (MQ=255)

                                                                                                                                  ggAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGt                 <  1:173613/150‑1 (MQ=255)

                                                                                                                                   gAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTc                >  2:1999701/1‑150 (MQ=255)

                                                                                                                                         gccggcTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTT‑TTTAATGATTTAACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACg          >  1:1970954/1‑150 (MQ=255)

                                                                                                                                              cTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGt     <  2:747394/150‑1 (MQ=255)

                                                                                                                                                cccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGTgc   <  2:2192782/150‑1 (MQ=255)

                                                                                                                                                 ccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAggg                           >  2:1747173/1‑125 (MQ=255)

                                                                                                                                                 ccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAggg                           <  1:1747173/125‑1 (MQ=255)

                                                                                                                                                 ccTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTATTCACGGGCGTgcg  >  1:2404232/1‑150 (MQ=255)

CATGGCCTTCATGCCTGACTACCAGATCCTGGTCATTTCCACCGGTATCGCTGCGGCGGTCGTGGTGGCGATGGGTGTGGTGCACCAGATCCGCTCTGGTAGCAAGCAGCACTAAGCGTCACTCGCCTCTGGAAACGGCCGGCTCCCTTGGGG‑‑ACCCGGCCGTTTCGCGTTGTTGGGC‑CTGGGCTT‑TTTCTGCGGGCATGTGGAAACGCTGGTTCATCCACGGTG‑AACCGAGCAGGGCTGCCGCAGCGATCAGGGCCAGGATGGCGAGGGCGATGTAGGTCTTCACGGGCGTGCG  >  NC_002947/2890334‑2890628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`