| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,008,361 | C→T | 7.0% | S259S (TCG→TCA) | PP_2628 ← | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,008,361 | 0 | C | T | 7.0% | 166.1 / 2.8 | 57 | S259S (TCG→TCA) | PP_2628 | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (22/31); new base T (2/2); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
AACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGATGAA > NC_002947/3008212‑3008507 | aaCTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATcgac < 2:1928688/150‑1 (MQ=255)aaCTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGAGATAGCAGTTTTACCGTGGTGATGTTGGTGTAAGCGGCAGAAACCCGCCCGGTCATcgac > 1:2231733/1‑150 (MQ=255) tGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCgacgac > 1:1494029/1‑150 (MQ=255) gCTCACCAGGCGCATCTGGCGAAAATAGGTCTGCTTGAACTCCTGAATGCTAGAGCGAGCAACGTGAGCTTCGCGGTTGGAGTGCTAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCg < 2:1705996/150‑1 (MQ=255) cTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCgg < 2:412521/150‑1 (MQ=255) aCCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCa > 1:1479020/1‑150 (MQ=255) ccAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCAt > 2:1353761/1‑150 (MQ=255) cAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCAtt < 1:57940/150‑1 (MQ=255) aGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGCTCATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTg < 1:1357409/150‑1 (MQ=255) gcgcATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGc < 2:1494029/150‑1 (MQ=255) aTCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGc < 2:1876186/150‑1 (MQ=255) aaaCCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGAcc < 2:1304789/127‑1 (MQ=255) aaaCCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGAcc > 1:1304789/1‑127 (MQ=255) gTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTg < 2:1563883/150‑1 (MQ=255) tGCTTGAAATCTTCCATGGCTGCGCTCGCAAAGTTGGCGTCGCGGTTGGAGTGCGAAAACAGTCTTACCGTTGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGACCGACCTTGccc > 2:1331036/1‑150 (MQ=255) tGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCGTCGATGACCGGGc < 2:329718/122‑1 (MQ=255) tGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCGTCGATGACCGGGc > 1:329718/1‑122 (MQ=255) cTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAg > 1:231456/1‑150 (MQ=255) aaaTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCgg < 1:2364650/150‑1 (MQ=255) aaaTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCgg < 1:502459/150‑1 (MQ=255) aaTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCggg < 2:289243/150‑1 (MQ=255) aaTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCggg > 1:289243/1‑150 (MQ=255) aTCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCgggg < 1:959132/150‑1 (MQ=255) tCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCt > 1:1286722/1‑127 (MQ=255) tCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCt < 2:1286722/127‑1 (MQ=255) aTGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGaaa > 1:1585367/1‑150 (MQ=255) aTGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGaaa < 2:503582/150‑1 (MQ=255) gCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGAc > 2:2428318/1‑143 (MQ=255) gCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGAc < 1:2428318/143‑1 (MQ=255) cgcgcAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATg < 2:1585367/150‑1 (MQ=255) aaGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAg < 2:1597018/150‑1 (MQ=255) aGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGc < 2:305136/150‑1 (MQ=255) tGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGaaa > 2:1279830/1‑132 (MQ=255) tGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGaaa < 1:1279830/132‑1 (MQ=255) aaaaCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGa < 2:949001/150‑1 (MQ=255) aaaCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGaa < 2:231456/150‑1 (MQ=255) aaaCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGaa < 1:693710/150‑1 (MQ=255) aCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGAc < 2:1340793/103‑1 (MQ=255) aCAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGAc > 1:1340793/1‑103 (MQ=255) cAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAggg < 1:1582502/150‑1 (MQ=255) aGTTTTACCGTGGTGATGTTGGTGTAAGCGTAAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGgc < 1:1601868/150‑1 (MQ=255) aCCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACTACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATc > 2:2047767/1‑150 (MQ=255) cGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAg < 1:1353761/150‑1 (MQ=255) tgATGTTGGTGTAAGCGACAGAAACCCGCCCGGTCAGCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCa > 2:1970273/1‑150 (MQ=255) gATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACa < 2:252769/95‑1 (MQ=255) gATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACa > 1:252769/1‑95 (MQ=255) gtgtAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAg > 2:610452/1‑150 (MQ=255) tAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGgcagca < 1:801020/130‑1 (MQ=255) tAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGgcagca > 2:801020/1‑130 (MQ=255) gTCNGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGc > 2:1514361/1‑150 (MQ=255) cAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAgg > 2:939595/1‑108 (MQ=255) cAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAgg < 1:939595/108‑1 (MQ=255) aCCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTc < 2:1903575/126‑1 (MQ=255) aCCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTc > 1:1903575/1‑126 (MQ=255) gcccgGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCa > 2:823308/1‑90 (MQ=255) gcccgGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCa < 1:823308/90‑1 (MQ=255) cccgGTCGTCGATGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAg < 1:854682/97‑1 (MQ=255) cccgGTCGTCGATGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCAAg > 2:854682/1‑97 (MQ=255) ccgGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACGCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATc > 1:26054/1‑150 (MQ=255) tCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGATGa > 1:605145/1‑150 (MQ=255) cgacgaCCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGATGaa < 2:605145/150‑1 (MQ=255) gacgacCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGAt < 2:1877695/146‑1 (MQ=255) gacgacCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGAt > 1:1877695/1‑146 (MQ=255) | AACTGGCTCACCAGGCGCATCTGGCGAAAACCGGTCTGCTTGAAATCTTCCATGGCTGCGCGCGCAAAGTGCGCTTCGCGGTTGGAGTGCGAAAACAGTTTTACCGTGGTGATGTTGGTGTAAGCGTCAGAAACCCGCCCGGTCATCGACGACCGGGCATTGGCCTGCTCCTGCCCGACCTTGCCCAGCCGGGGGACGAAATACAGCATGGCCAAGCCGAACAGCACGACCCAGGCAATGAAGGGCAGCATCAGTTTCAGGGCAAAGCCGCCGGCCAGGGCAATGATCGCGATGAA > NC_002947/3008212‑3008507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |