Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,077,158 G→T 7.7% A246A (GCC→GCA)  PP_2687 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,077,1580GT 7.7% 282.7 / 9.4 92A246A (GCC→GCA) PP_2687hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (47/37);  new base T (2/5);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01

TCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGGGCGGA  >  NC_002947/3077009‑3077307
                                                                                                                                                     |                                                                                                                                                      
tCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAg                                                                                                                                                        <  1:709384/150‑1 (MQ=255)
tCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAg                                                                                                                                                        <  1:717253/150‑1 (MQ=255)
tCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAg                                                                                                                                                        <  1:1544532/150‑1 (MQ=255)
  gCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGc                                                                                                                                                      >  1:697433/1‑150 (MQ=255)
  gCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGc                                                                                                                                                      >  2:2005083/1‑150 (MQ=255)
   cTTAGCCAGGGATGCAACCCTGCATCGAGGGAGTGGGCGTGTGCGGGGTGGGGCATGCGGGCGTTCAGGTGGGAGCACCTGCTAGGTCAGTCTCGTCCTGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCa                                                                                                                                                     >  1:1751237/1‑150 (MQ=255)
    ttAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCTGTTCCTCGATGCAGGCAt                                                                                                                                                    <  1:81694/150‑1 (MQ=255)
    ttAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCAt                                                                                                                                                    <  2:2365115/150‑1 (MQ=255)
        ccAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCag                                                                                                                                                >  2:1186889/1‑150 (MQ=255)
        ccAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCag                                                                                                                                                >  1:202708/1‑150 (MQ=255)
         cAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagc                                                                                                                                               >  1:1673198/1‑150 (MQ=255)
           gggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCGTGCGCTGGGCGGTACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCagcga                                                                                                                                             <  1:1989655/150‑1 (MQ=255)
            ggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgag                                                                                                                                            <  1:2297467/150‑1 (MQ=255)
            ggATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGCTATCTTTTGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgag                                                                                                                                            <  2:2433251/150‑1 (MQ=255)
                gCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGcgcg                                                                                                                                        >  1:1079430/1‑150 (MQ=255)
                gCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGcgcg                                                                                                                                        <  1:1699932/150‑1 (MQ=255)
                  aaCCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCTTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgaggaactg                                                                                                                                      <  2:100667/150‑7 (MQ=255)
                      cTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcga                                                                                                                                             >  1:1669799/1‑139 (MQ=255)
                      cTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcga                                                                                                                                             <  2:1669799/139‑1 (MQ=255)
                         cagcGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGGGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGACTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCa                                                                                                                              <  1:764705/147‑1 (MQ=255)
                          aTCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCAc                                                                                                                             <  2:1673198/150‑1 (MQ=255)
                               ggATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCa                                                                                                                        >  2:1556581/1‑150 (MQ=255)
                                      gtcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAAcagcag                                                                                                                 <  1:2005083/150‑1 (MQ=255)
                                      gtcgATGGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCCGCCAGGATCAGGTCCAGTTCCTCGATGCCGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAAcagcag                                                                                                                 <  2:122710/150‑1 (MQ=255)
                                       tcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACagcagc                                                                                                                >  1:566191/1‑150 (MQ=255)
                                       tcgATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACagcagc                                                                                                                >  2:252897/1‑150 (MQ=255)
                                            tGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGTTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTc                                                                                                           <  2:566191/150‑1 (MQ=255)
                                                     gcgcAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCGCGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACAccgg                                                                                                  >  1:555591/1‑150 (MQ=255)
                                                         aGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACAccggccgg                                                                                              >  2:1951142/1‑150 (MQ=255)
                                                             gcgcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATg                                                                                                                                                   <  2:744628/94‑1 (MQ=255)
                                                             gcgcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATg                                                                                                                                                   >  1:744628/1‑94 (MQ=255)
                                                               gcgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTg                                                                                        <  2:264701/150‑1 (MQ=255)
                                                                cgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTgg                                                                                       <  1:396175/150‑1 (MQ=255)
                                                                cgTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGGCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTgg                                                                                       <  2:710451/150‑1 (MQ=255)
                                                                 gTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCGGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTggg                                                                                      >  1:1879154/1‑150 (MQ=255)
                                                                 gTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTggg                                                                                      >  2:1200447/1‑150 (MQ=255)
                                                                  ttCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGt                                                                                     >  2:1652528/1‑150 (MQ=255)
                                                                  ttCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGt                                                                                     >  2:2447998/1‑150 (MQ=255)
                                                                         cggcggCCATGCGCTGGGCGGCACGCCCGGTACTGCCCCGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGgcgc                                                                              <  1:623780/150‑1 (MQ=255)
                                                                           gcggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGa                                                                            <  1:2447998/150‑1 (MQ=255)
                                                                             ggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATa                                                                          <  2:955664/150‑1 (MQ=255)
                                                                             ggcCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATa                                                                          <  1:252897/150‑1 (MQ=255)
                                                                                    cgcTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgagga                                                                                                                                          <  1:684706/80‑3 (MQ=37)
                                                                                    cgcTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTGCATGCCTGCatcgagga                                                                                                                                          >  2:684706/1‑78 (MQ=37)
                                                                                    cgcTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCg                                                                   <  2:1089615/150‑1 (MQ=255)
                                                                                      cTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                                 <  2:697433/150‑1 (MQ=255)
                                                                                      cTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                                 <  2:1068069/150‑1 (MQ=255)
                                                                                       tGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTgcg                                                                                  >  2:288263/1‑132 (MQ=255)
                                                                                       tGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCATCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTgcg                                                                                  <  1:288263/132‑1 (MQ=255)
                                                                                         ggcggcACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGaa                                                              <  2:1934768/150‑1 (MQ=255)
                                                                                             gcACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCt                                                          <  2:884071/150‑1 (MQ=255)
                                                                                               aCGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGCTCCAGTCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTc                                                        >  1:2132501/1‑150 (MQ=255)
                                                                                                cGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTACAGTTCCTCGATGCAAGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCg                                                       >  2:1044467/1‑150 (MQ=255)
                                                                                                 gCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGt                                                      >  2:280729/1‑150 (MQ=255)
                                                                                                 gCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGt                                                      <  1:1556581/150‑1 (MQ=255)
                                                                                                     ggTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGa                                                  <  2:426236/150‑1 (MQ=255)
                                                                                                     ggTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGa                                                  <  1:2234267/150‑1 (MQ=255)
                                                                                                         cTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTc                                              >  2:1532281/1‑150 (MQ=255)
                                                                                                          tGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCa                                             >  2:2387305/1‑150 (MQ=255)
                                                                                                          tGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGACCGCGG‑CACGTCCACCCGCAACAACAGCAACTCGGACACCGGCCGGGCCCTGGGTGCGGCGCGATAGATCTCGGTGAAGGCTTCGTACgctggcc                                             >  1:531451/1‑144 (MQ=255)
                                                                                                            cctggCAGGCCTGCCGGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGAAACCACACGGCTAACGTCCGGTCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAgc                                           >  1:281366/4‑150 (MQ=255)
                                                                                                             ccGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAgcg                                          <  1:1094107/150‑1 (MQ=255)
                                                                                                                      cTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCgcagca                                 >  2:1078963/1‑150 (MQ=255)
                                                                                                                       tGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGcagcag                                >  1:227337/1‑150 (MQ=255)
                                                                                                                        gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAg                                                 <  2:2216704/132‑1 (MQ=255)
                                                                                                                        gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAg                                                 >  1:2216704/1‑132 (MQ=255)
                                                                                                                        gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAgg                               >  2:865/1‑150 (MQ=255)
                                                                                                                        gCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAgg                               <  1:475602/150‑1 (MQ=255)
                                                                                                                         ccAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAggg                              >  2:691606/1‑150 (MQ=255)
                                                                                                                          cAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCACGCGCAGCAGGgc                             >  2:1292255/1‑150 (MQ=255)
                                                                                                                             gATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGa                                                               >  1:1413284/1‑113 (MQ=255)
                                                                                                                             gATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGa                                                               <  2:1413284/113‑1 (MQ=255)
                                                                                                                                  ggTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCAtt                     >  1:345885/1‑150 (MQ=255)
                                                                                                                                   gTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATCTTGCACCTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTg                    <  1:1995672/150‑1 (MQ=255)
                                                                                                                                   gTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTg                    <  1:1652528/150‑1 (MQ=255)
                                                                                                                                   gTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTg                    >  1:1599375/1‑150 (MQ=255)
                                                                                                                                    tCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTgg                   >  2:1137901/1‑150 (MQ=255)
                                                                                                                                     ccAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGt                  <  1:824340/150‑1 (MQ=255)
                                                                                                                                        gTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGtaat               <  1:1044467/150‑1 (MQ=255)
                                                                                                                                         ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                                 >  1:1541478/1‑99 (MQ=255)
                                                                                                                                         ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGtt                                                                 <  2:1541478/99‑1 (MQ=255)
                                                                                                                                         ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGgc                             >  2:2011297/1‑135 (MQ=255)
                                                                                                                                         ttCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGgc                             <  1:2011297/135‑1 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTGCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCg                                                       >  2:1345386/1‑106 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCg                                                       <  1:1345386/106‑1 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc           >  1:778709/1‑150 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc           >  2:316069/1‑150 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc           >  2:388124/1‑150 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc           >  2:526770/1‑150 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc           >  2:889079/1‑150 (MQ=255)
                                                                                                                                            cTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTAAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAAcc           >  2:1694838/1‑150 (MQ=255)
                                                                                                                                             tCGATGTAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg          >  2:577855/1‑150 (MQ=255)
                                                                                                                                             tCGATGCAGGCATGCAGCGAGCGCGGACAGAACCA‑CCGCAACAGCAGCACCTTAGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg          >  1:299870/1‑150 (MQ=255)
                                                                                                                                             tCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGTATCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACcg          >  1:2399433/1‑150 (MQ=255)
                                                                                                                                                  gCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCgcgggc     >  2:2119017/1‑150 (MQ=255)
                                                                                                                                                     ggCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGGGCGGa  <  2:778709/150‑1 (MQ=255)
                                                                                                                                                     ggCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGGGCGGa  <  1:316069/150‑1 (MQ=255)
                                                                                                                                                     ggCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGGGCGGa  <  2:1984117/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TCGCTTAGCCAGGGATGCAACCCTGCATCGAGGATTTCGTCGATTGCGGTGTAGCGCAGGCGCGCGTTCAGTTCGGCGGCCATGCGCTGGGCGGCACGCCCGGTACTGCCCGGCAGGCCTGCCAGGATCAGGTCCAGTTCCTCGATGCAGGCATGCAGCGAGCGCGG‑CACGTCCACCCGCAACAGCAGCAACTCGGACACCGGCCGGGCGCTGGGTGCGGCGCGATAGAGCTCGTTGAAGGCTTCGTACGAGGTCAGCGCGCGCAGCAGGGCGCTCCATTGGTAATAACCGCGGGCGGA  >  NC_002947/3077009‑3077307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑