Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,282,331	A→G	5.5%	L26L (CTT→CTC)	PP_2883 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,282,331	0	A	G	5.5%	280.3 / 3.8	91	L26L (CTT→CTC)	PP_2883	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (48/38);  new base G (3/2);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CTGCCAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGC  >  NC_002947/3282184‑3282480                                                                                                                                                    |                                                                                                                                                       cTGCCAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAg                                                                                                                                                      <  1:329941/150‑1 (MQ=255)    ccAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAggtg                                                                                                                                                   <  1:1790250/150‑1 (MQ=255)     cAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAggtgg                                                                                                                                                  <  1:1916420/150‑1 (MQ=255)      aGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAggtgg‑t                                                                                                                                                >  1:1413142/1‑150 (MQ=255)          ttCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCA‑CCACCTTGAGGTGGCTGCAc                                                                                                                                            <  1:1249037/150‑1 (MQ=255)                gccggccTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAg                                                                                                                                      >  2:2046316/1‑150 (MQ=255)                 ccggccTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCGCCTCAAGGTGG‑TGCACGCCAAgg                                                                                                                                     <  2:1192757/150‑1 (MQ=255)                   ggccTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGc                                                                                                                                   <  1:772776/150‑1 (MQ=255)                   ggccTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGc                                                                                                                                   <  2:1120252/150‑1 (MQ=255)                    gccTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGcc                                                                                                                                  >  1:1666089/1‑150 (MQ=255)                      cTGTTCGCCGTCGGGGGCTTGGCAACGATTGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCcac                                                                                                                                <  1:1077210/150‑1 (MQ=255)                      cTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAggtg                                                                                                                                                   >  1:69841/1‑132 (MQ=255)                      cTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAggtg                                                                                                                                                   <  2:69841/132‑1 (MQ=255)                      cTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCcac                                                                                                                                >  2:2093487/1‑150 (MQ=255)                              cGTCGGGGGCGTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCa                                                                                                                        <  2:2203859/150‑1 (MQ=255)                               gTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAg                                                                                                                       <  2:1490736/150‑1 (MQ=255)                               gTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAg                                                                                                                       <  1:1924808/150‑1 (MQ=255)                                      gCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTgg                                                                                                                >  2:1177210/1‑150 (MQ=255)                                        ttGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGAACAACAAGGGCCACATTGCGCAGGCACTGGTa                                                                                                              <  1:1881952/150‑1 (MQ=255)                                             aacgaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACAt                                                                                                         >  2:491323/1‑150 (MQ=255)                                             aacgaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACAt                                                                                                         <  1:914294/150‑1 (MQ=255)                                                gaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATccc                                                                                                      >  1:2323096/1‑150 (MQ=255)                                                  acgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCNCgg                                                                                                    <  2:1496884/150‑1 (MQ=255)                                                            ccAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCCGGGATTATGCTCAAGGTGG‑TGCACGCCAAGGGCCACCTTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTg                                                                                          >  1:2253630/1‑150 (MQ=255)                                                                   ttCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCt                                                                                   >  2:195948/1‑150 (MQ=255)                                                                   ttCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCt                                                                                   >  1:2176457/1‑150 (MQ=255)                                                                    tCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCtg                                                                                  <  1:195948/150‑1 (MQ=255)                                                                      ggTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCtgtg                                                                                >  2:286861/1‑150 (MQ=255)                                                                        tATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTggg                                                                              >  2:45928/1‑150 (MQ=255)                                                                          tCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTc                                                                            >  1:574721/1‑150 (MQ=255)                                                                            cAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTa                                                                                                              >  2:1982870/1‑114 (MQ=255)                                                                            cAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTa                                                                                                              <  1:1982870/114‑1 (MQ=255)                                                                            cAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCaa                                                                          >  1:2312970/1‑150 (MQ=255)                                                                             aGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAg                                                                         >  2:613865/1‑150 (MQ=255)                                                                              gggCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCaata                                                                        <  2:2312970/150‑3 (MQ=255)                                                                                gCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATc                                                                      >  1:670912/1‑150 (MQ=255)                                                                                 ccAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCCCTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCa                                                                     >  2:807881/1‑150 (MQ=255)                                                                                 ccAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCa                                                                     <  2:2323096/150‑1 (MQ=255)                                                                                  cAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAggg                                                                                                                                    <  1:1307658/86‑1 (MQ=255)                                                                                  cAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAggg                                                                                                                                    >  2:1307658/1‑86 (MQ=255)                                                                                       tCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGTAGTCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAAtt                                                               >  1:61373/1‑150 (MQ=255)                                                                                        cAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTc                                                              <  2:1666089/150‑1 (MQ=255)                                                                                        cAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTc                                                              <  1:1160751/150‑1 (MQ=255)                                                                                          gTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGc                                                            <  1:2046316/150‑1 (MQ=255)                                                                                             gCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCgg                                                         >  1:85148/1‑150 (MQ=255)                                                                                              ccAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGc                                                        >  2:1289361/1‑150 (MQ=255)                                                                                               cAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCg                                                       >  1:40305/1‑150 (MQ=255)                                                                                                aGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCgt                                                      >  2:1856482/1‑150 (MQ=255)                                                                                                aGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCgt                                                      <  2:574721/150‑1 (MQ=255)                                                                                                 gCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCgtg                                                     >  1:124799/1‑150 (MQ=255)                                                                                                  cccTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTgg                                                                                                                <  2:675519/90‑1 (MQ=255)                                                                                                  cccTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTgg                                                                                                                >  1:675519/1‑90 (MQ=255)                                                                                                     tGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTgg                                                 >  1:1110580/1‑150 (MQ=255)                                                                                                          gCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtg                                            <  2:124799/150‑1 (MQ=255)                                                                                                            cGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCg                                                                                                     >  1:390768/1‑91 (MQ=255)                                                                                                            cGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCg                                                                                                     <  2:390768/91‑1 (MQ=255)                                                                                                            cGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtgat                                          <  1:1565811/150‑1 (MQ=255)                                                                                                            cGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtgat                                          >  1:1165997/1‑150 (MQ=255)                                                                                                               cATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACTCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTgatgat                                       >  2:1350849/1‑150 (MQ=255)                                                                                                                 tCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGAtct                                     >  1:1690122/1‑150 (MQ=255)                                                                                                                   gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtga                                           <  2:2297494/142‑1 (MQ=255)                                                                                                                   gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtga                                           <  2:2297151/142‑1 (MQ=255)                                                                                                                   gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtga                                           >  1:2297494/1‑142 (MQ=255)                                                                                                                   gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCtga                                           >  1:2297151/1‑142 (MQ=255)                                                                                                                   gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCg                                   >  2:569065/1‑150 (MQ=255)                                                                                                                   gATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCg                                   <  1:579724/150‑1 (MQ=255)                                                                                                                    tgcacgccgcgATGGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCgg                                  >  1:2108950/6‑150 (MQ=255)                                                                                                                     tCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGc                                 <  2:2253630/150‑1 (MQ=255)                                                                                                                       aGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCt                               >  2:2183502/1‑150 (MQ=255)                                                                                                                        ggCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTc                              >  1:196930/1‑150 (MQ=255)                                                                                                                         gccgccATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCCAGGGCCACATTGCGGAGGCACTGGTAGACATCCCGGGCAATCGGGGAACTGCTGTGGGGCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCa                             >  2:865350/1‑150 (MQ=255)                                                                                                                          ccgccATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTAAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTGAt                            >  1:1111355/1‑150 (MQ=255)                                                                                                                              gcccttggcgTGCA‑CCACCTTGAGGTGGCTGCACGCCGAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCgg                        >  1:2394942/7‑150 (MQ=38)                                                                                                                                tggcggcgTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGc                      <  2:2108950/148‑1 (MQ=255)                                                                                                                                ccttggcgTGCA‑CCACCTTGAGGTGGCTGCACGCCGAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAg                                                                    >  2:957652/5‑104 (MQ=25)                                                                                                                                ccttggcgTGCA‑CCACCTTGAGGTGGCTGCACGCCGAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAg                                                                    <  1:957652/100‑1 (MQ=25)                                                                                                                                ccttggcgTGCA‑CCACCTTGAGGTGGCTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGc                      >  2:2261466/5‑150 (MQ=255)                                                                                                                                 cgcgGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCGCATCCGGGcc                     <  2:1165997/150‑1 (MQ=255)                                                                                                                                   cggcgTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGa                   <  2:670912/150‑1 (MQ=255)                                                                                                                                    ggcgTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGAt                  >  1:1407804/1‑150 (MQ=255)                                                                                                                                         gCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGAt                  <  2:1927101/145‑1 (MQ=255)                                                                                                                                         gCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGAt                  >  1:1927101/1‑145 (MQ=255)                                                                                                                                          cAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCtt            <  1:613865/150‑1 (MQ=255)                                                                                                                                               aCCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGc                      >  2:294760/1‑135 (MQ=255)                                                                                                                                               aCCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGc                      <  1:294760/135‑1 (MQ=255)                                                                                                                                               aCCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAAc       >  1:1846143/1‑150 (MQ=255)                                                                                                                                               aCCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAAc       >  2:2220886/1‑150 (MQ=255)                                                                                                                                                 cTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTc                                                                            <  1:1110620/79‑1 (MQ=255)                                                                                                                                                 cTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTc                                                                            >  2:1110620/1‑79 (MQ=255)                                                                                                                                                 cTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACtg     >  1:1025778/1‑150 (MQ=255)                                                                                                                                                 cTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACtg     >  1:479908/1‑150 (MQ=255)                                                                                                                                                    aaGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGc  <  1:936961/150‑1 (MQ=255)                                                                                                                                                    aaGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGc  >  2:1690614/1‑150 (MQ=255)                                                                                                                                                    aaGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACGGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGc  <  2:670250/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       CTGCCAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGG‑TGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTCGGCCTCATCCGGGCCGATGTTCTTGAAACTGTGC  >  NC_002947/3282184‑3282480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑