| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 5,914,046 | +A | coding (1259/1377 nt) | spuI → | glutamylpolyamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,914,044 | 1 | . | A | 97.1% | 105.6 / NA | 34 | L419L (CTG→CTA) | spuI | glutamylpolyamine synthetase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base A (15/18); minor base C (0/1); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑AACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAG > NC_002947/5913900‑5914185 | cGTACTGGCTAGCGTGCACCATGGCCTGACCAACACGATCGCGCCAGGCGCGCCCATCGAAGGCAACTCGTCCGAGCAGATGGAGCAGAGACTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aaaca > 2:12155/1‑147 (MQ=21) gTACTGGCTGGCATGCCCCATGGCAAGGCCAACTTGTTCTAGCCAGGAGCGCCGATCGACGGCAGATGCACCAATCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aaacaa < 1:452488/150‑5 (MQ=12) ccATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGt > 1:508118/1‑150 (MQ=255) cATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTa > 1:535902/1‑150 (MQ=255) aTGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTAc > 1:338570/1‑150 (MQ=255) ggCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACAt < 1:199806/150‑1 (MQ=255) tGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAg > 2:154815/1‑141 (MQ=255) tGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAg < 1:154815/141‑1 (MQ=255) caacaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCt < 1:733901/150‑1 (MQ=255) acaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTtc < 2:508118/150‑1 (MQ=255) ccAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGGACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCaa < 1:824290/150‑1 (MQ=255) aGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAgg < 1:650736/150‑1 (MQ=255) gcgcgcCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGaa < 1:573108/150‑1 (MQ=255) cgcgcCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGaaa < 2:586154/150‑1 (MQ=255) aTCGAAGGCAACGCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCTACATCTTCGTCGCGTGCAAGGAAAGCGAGCt < 2:135413/150‑1 (MQ=255) aaGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGaa > 1:486496/1‑150 (MQ=255) aGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAag > 1:659028/1‑150 (MQ=255) cAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGt < 2:486496/150‑1 (MQ=255) aaCTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGtt > 2:211355/1‑150 (MQ=255) aCTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTc < 2:802482/150‑1 (MQ=255) tCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGa < 1:423464/150‑1 (MQ=255) gTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGc > 1:128756/1‑150 (MQ=255) gcagcTGGAGCAGAGCCTGCCGAACAACCTGCGCGACGCCCTGCGCGAGCTGGCCGTCAGCTAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTTGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGa < 1:361928/150‑1 (MQ=25) gAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGa < 1:211355/150‑1 (MQ=255) gCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGAcc > 1:340880/1‑150 (MQ=255) ccTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGa < 2:612275/142‑1 (MQ=255) ccTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGa > 1:612275/1‑142 (MQ=255) gCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACa > 1:759104/1‑150 (MQ=255) aacaacCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTa > 2:407127/1‑57 (MQ=18) aacaacCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTa < 1:407127/57‑1 (MQ=18) tGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTg > 1:418848/1‑150 (MQ=255) tGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTg > 2:539119/1‑150 (MQ=255) gcgATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGCACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCAt > 1:364133/1‑150 (MQ=255) gCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGATATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCgt < 1:12155/150‑1 (MQ=255) gacAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACgccgcc < 2:659028/150‑1 (MQ=255) agaTCCTGCAACTACTCCATCCATCCGAAGTACCTCGAAATCTTCGTCGCGGGCAAGGAAAGCGAGCTGGAATAGTTCGAGAACTCAATCGCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAg < 1:126964/150‑1 (MQ=37) | CGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑AACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAG > NC_002947/5913900‑5914185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |