Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,852,005	G→A	R350H (CGT→CAT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,852,005	0	G	A	100.0%	126.9 / NA	38	R350H (CGT→CAT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/18);  total (20/18)

GGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGC  >  NC_002947/1851868‑1852150                                                                                                                                          |                                                                                                                                                   gggCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCAt                                                                                                                                        <  2:231944/150‑1 (MQ=255)      tCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACACACGGCATGCACg                                                                                                                                   >  1:469138/1‑150 (MQ=255)        gTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCTCGaa                                                                                                                                 <  2:469138/150‑1 (MQ=255)          gCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAaga                                                                                                                               >  2:151494/1‑150 (MQ=255)             cacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGc                                                                                                                            >  2:793131/1‑150 (MQ=255)                                  cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCa                                                                                                       >  1:147543/1‑150 (MQ=255)                                       aGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATACATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                                  <  1:261760/150‑1 (MQ=255)                                        gTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcg                                                                                                 <  1:736348/150‑1 (MQ=255)                                                   aCATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                                  >  1:469185/1‑138 (MQ=255)                                                   aCATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                                  <  2:469185/138‑1 (MQ=255)                                                    cATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAc                                                                                     >  2:576390/1‑150 (MQ=255)                                                     aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAc‑c                                                                                   >  2:5778/1‑150 (MQ=255)                                                          cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGt                                                                              >  2:550044/1‑150 (MQ=255)                                                             cccaAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCaa                                                                           <  2:347623/150‑1 (MQ=255)                                                                            cTACCGCTCGTTGCACACTGCGGGAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCa                                                            >  2:773350/1‑150 (MQ=255)                                                                             tACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCAc                                                           >  2:180137/1‑150 (MQ=255)                                                                              aCCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACt                                                          >  2:275718/1‑150 (MQ=255)                                                                               ccacTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTa                                                         >  1:792716/4‑150 (MQ=255)                                                                                            acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTc                                                                             <  2:77109/117‑1 (MQ=255)                                                                                            acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTc                                                                             >  1:77109/1‑117 (MQ=255)                                                                                              tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                          <  1:62375/150‑1 (MQ=255)                                                                                                    aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATGAATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCACGCACAGCTCCAACCACTACGAAGAAAAGATTTccgtgtt                                    >  1:142384/1‑145 (MQ=255)                                                                                                    aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGtt                                    <  1:550044/150‑1 (MQ=255)                                                                                                    aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGtt                                    <  2:690088/150‑1 (MQ=255)                                                                                                      tCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGc                                  <  1:5778/150‑1 (MQ=255)                                                                                                        ggCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGt                                <  1:576390/150‑1 (MQ=255)                                                                                                         gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                               <  1:773350/150‑1 (MQ=255)                                                                                                          ccccGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCa                              <  1:275718/150‑1 (MQ=255)                                                                                                            ccGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAg                                                                                          <  1:669957/89‑1 (MQ=255)                                                                                                            ccGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAg                                                                                          >  2:669957/1‑89 (MQ=255)                                                                                                                     aGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGt                   <  1:180137/150‑1 (MQ=255)                                                                                                                      ggTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg                  >  1:521336/1‑150 (MQ=255)                                                                                                                       gTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAt                                             <  1:596035/122‑1 (MQ=255)                                                                                                                       gTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAt                                             >  2:596035/1‑122 (MQ=255)                                                                                                                           tGGAGGTGCAGATCCATACCCACGGCATGCACGAATAGTCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACg             >  1:455771/1‑150 (MQ=255)                                                                                                                               ggTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAaga         >  1:358670/1‑150 (MQ=255)                                                                                                                                   cAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTAC‑ACACCACGCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTg     <  1:151494/150‑1 (MQ=255)                                                                                                                                      aTCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGc  >  1:439341/1‑150 (MQ=255)                                                                                                                                          |                                                                                                                                                   GGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAC‑CGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGC  >  NC_002947/1851868‑1852150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑