Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 6,180,730 C→G W258S (TGG→TCG)  atpB ← ATP synthase subunit a

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,180,7300CG100.0% 92.0 / NA 29W258S (TGG→TCG) atpBATP synthase subunit a
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (16/13);  total (16/13)

CCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGATCGGCTTGGCGATC  >  NC_002947/6180586‑6180870
                                                                                                                                                |                                                                                                                                            
ccTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGcc                                                                                                                                         >  2:667752/1‑150 (MQ=255)
 cTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGccc                                                                                                                                        <  2:124717/150‑1 (MQ=255)
 cTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGccc                                                                                                                                        <  1:852810/150‑1 (MQ=255)
                               gACACGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGcc                                                                                                          >  2:857900/1‑150 (MQ=255)
                                acacGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCca                                                                                                         >  1:306581/1‑150 (MQ=255)
                                       aGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCcac                                                                                                        <  2:79606/144‑1 (MQ=255)
                                       aGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCcac                                                                                                        >  1:79606/1‑144 (MQ=255)
                                           ggTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCgg                                                                                              >  1:342007/1‑150 (MQ=255)
                                                 aaTGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGa                                                                                        >  1:614807/1‑150 (MQ=255)
                                                              gTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGAt                                                                           >  2:523554/1‑150 (MQ=255)
                                                                 aGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAg                                                                        <  1:858966/150‑1 (MQ=255)
                                                                     aTCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATg                                                                    <  2:306581/150‑1 (MQ=255)
                                                                             gTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGAc                                                                                                                                            <  1:530428/70‑1 (MQ=255)
                                                                                  cGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGc                                                       <  2:342007/150‑1 (MQ=255)
                                                                                    aTGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCa                                                     >  1:606417/1‑150 (MQ=255)
                                                                                                    aGATGAAAGCTTGCAGGGTGATGGTCAGGATGTGGAACACAGCCGACGCCCATTGCAGCAAGACAGCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGa                                     >  2:496616/1‑150 (MQ=255)
                                                                                                      aTGAAAGCGTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAAc                                   >  2:819936/1‑150 (MQ=255)
                                                                                                       tGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGc                                                                                                           >  1:72792/1‑77 (MQ=255)
                                                                                                       tGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGc                                                                                                           <  2:72792/77‑1 (MQ=255)
                                                                                                       tGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACa                                  <  1:857900/150‑1 (MQ=255)
                                                                                                                  aGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGc                                                                                                           <  2:329354/66‑1 (MQ=255)
                                                                                                                  aGGGTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGc                                                                                                           >  1:329354/1‑66 (MQ=255)
                                                                                                                    ggTGATGATCAGGATGTGGAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAg                     >  1:185419/1‑150 (MQ=255)
                                                                                                                               ggATGTGGAACACCGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGATCGGCt          >  2:368498/1‑150 (MQ=255)
                                                                                                                                  tgtgGAACACCGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGATCGGCTTgg       <  1:368498/150‑1 (MQ=255)
                                                                                                                                     ggAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGt                          <  2:426596/128‑1 (MQ=255)
                                                                                                                                     ggAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGt                          >  1:426596/1‑128 (MQ=255)
                                                                                                                                      gAACACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGATCGGCTTGGCGAt   <  1:523554/150‑1 (MQ=255)
                                                                                                                                       aaCACAGCCGACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGATCGGCTTGGCGATc  >  2:836441/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CCTCCCCCCGTACAGGCAGGGAAGGCTATCAGACACGCCAGGCGGTCTTAATGGTTATCTTCGTGAGCCATCGACAGGTAGACGATGGTCAGCATCATGAAGATGAAAGCTTGCAGGGTGATGATCAGGATGTGGAACACAGCCCACGCCCATTGCAGCACCACACCCAGGCCGCTGAGCCACAGCAGGCCGGAGCCGAACATTACAGCGATCAGGATGAACACCAGCTCGCCAGCATACATGTTGCCGAACAGACGCAGTGCCAGCGAGATCGGCTTGGCGATC  >  NC_002947/6180586‑6180870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑