| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,059,548 | Δ1 bp | 7.8% | coding (1334/1581 nt) | PP_4458 ← | opine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,059,548 | 0 | C | . | 7.8% | 153.7 / 7.9 | 51 | coding (1334/1581 nt) | PP_4458 | opine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (24/23); new base . (2/2); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
CCCAGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGT > NC_002947/5059402‑5059695 | cccAGACCTGTTTCAGACCAAACAAAGACATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGa > 1:870725/1‑150 (MQ=255) cAGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGaac < 1:360383/150‑2 (MQ=255) aGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAAt > 2:1101888/1‑150 (MQ=255) cTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑Ga > 2:572900/1‑150 (MQ=255) cTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTC‑gga < 2:743643/150‑3 (MQ=255) aCCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGctca > 1:604219/1‑147 (MQ=255) aCCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGT‑CGAATTTCGGACATTGctca < 2:604219/150‑4 (MQ=255) caaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTgc < 2:335559/150‑1 (MQ=255) aaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTgcg > 1:993151/1‑150 (MQ=255) gggATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCg < 1:659747/150‑1 (MQ=255) tGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATgg < 2:22694/150‑1 (MQ=255) aTACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTc > 1:440174/1‑119 (MQ=255) aTACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTc < 2:440174/119‑1 (MQ=255) gtcgtGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATc < 2:185288/150‑1 (MQ=255) cgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTc > 2:873811/1‑145 (MQ=255) cgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTc < 1:873811/145‑1 (MQ=255) cTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGc > 1:528711/1‑102 (MQ=255) cTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGc < 2:528711/102‑1 (MQ=255) tCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCgg < 1:1103437/150‑1 (MQ=255) acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGgcgc > 1:1110049/1‑150 (MQ=255) acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGATACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGt < 2:878372/133‑1 (MQ=255) acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGATACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGt > 1:878372/1‑133 (MQ=255) aaaTCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGccc < 2:440586/150‑1 (MQ=255) aaTCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGcc > 2:1027257/1‑113 (MQ=255) aaTCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGcc < 1:1027257/113‑1 (MQ=255) tCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTgcgc > 2:287479/1‑97 (MQ=255) tCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTgcgc < 1:287479/97‑1 (MQ=255) tCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCa > 2:700309/1‑124 (MQ=255) tCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCa < 1:700309/124‑1 (MQ=255) tGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAgcagca > 2:897402/1‑150 (MQ=255) gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAac > 2:610128/1‑150 (MQ=255) gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGGGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAac > 2:617178/1‑150 (MQ=255) ccTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAaca > 2:277194/1‑150 (MQ=255) tCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACg > 1:430467/1‑150 (MQ=255) aCCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GCCATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTATGTAAGAAAGGACTCGGGGTTCGGATATCGAGCAGCACCGTAAAACACGATc < 1:1101888/150‑1 (MQ=255) aCCCTGGCGTTCAGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGACGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATc > 1:294049/1‑150 (MQ=255) ccTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGACATTT‑GAAGTAGCAGCGGGTGCGCCGAGTGCAGAAGCAGAATCGTAAAACTCTGTGATCCGTGAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATcgc > 1:570489/1‑150 (MQ=255) ccTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATcgc > 1:147698/1‑150 (MQ=255) tGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCgcgc > 2:1017053/1‑150 (MQ=255) ggCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACTTTGCAGCGGGTGCGCCGATGGCAGAAGCCGCATCGTAAAACTCTGTCAGCCAATAGTCGGCGCCCGGATATCGAGCAGCACCGCAAAACACGATCGCGct < 2:570489/150‑1 (MQ=255) ggATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCtt > 1:1118567/1‑150 (MQ=255) atcatcAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCt < 1:610128/150‑1 (MQ=255) tcaGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAActct < 1:241576/75‑1 (MQ=255) tcaGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAActct > 2:241576/1‑75 (MQ=255) tcaGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTg < 2:506782/150‑1 (MQ=255) gCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGcc < 2:430467/150‑1 (MQ=255) aCTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTgg > 1:385757/1‑150 (MQ=255) gctgCAATGT‑CGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCCTGACGGCTTTTGGCCTGGTa > 2:885871/4‑150 (MQ=255) aTGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTcgc < 2:385757/150‑1 (MQ=255) tGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTcgcg < 1:617178/150‑1 (MQ=255) gTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGt < 2:147698/150‑1 (MQ=255) | CCCAGACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTC‑GACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAGTCGCGT > NC_002947/5059402‑5059695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |