Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,458,946 Δ1 bp 9.1% intergenic (+23/‑39) leuS → / → PP_4795 leucine‑‑tRNA ligase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,458,9460T. 9.1% 157.2 / 10.4 55intergenic (+23/‑39)leuS/PP_4795leucine‑‑tRNA ligase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base . (3/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

CGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGAC  >  NC_002947/5458806‑5459092
                                                                                                                                             |                                                                                                                                                  
cgAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTgg                                                                                                                                           <  2:483685/150‑1 (MQ=255)
 gaagaaGTAGCAGCCGCTGCCCGCAGCAACGAGAACGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTggg                                                                                                                                          <  1:227520/150‑1 (MQ=255)
      agTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGaa                                                                                                                                     <  1:181515/150‑1 (MQ=255)
      agTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGaa                                                                                                                                     <  1:196591/150‑1 (MQ=255)
       gTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAAc                                                                                                                                    >  1:501401/1‑150 (MQ=255)
         cGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCgttttca                                                                                                                                  <  2:1005118/150‑7 (MQ=255)
           aaGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGc                                                                                                                                <  2:663879/150‑1 (MQ=255)
           aaGCCGCTGCCCGCAGCAACGAGAATGTCCTGAGAATAACCTATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGc                                                                                                                                <  1:607813/150‑1 (MQ=255)
             gCCGCTGCCCGCAGCAACGAGAATGTCCTGAGCTTCATCGATGGCCTGACCATCCGCAAGGGCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGccc                                                                                                                              >  2:184194/1‑150 (MQ=255)
                   gCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCACACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAgg                                                                                                                        <  2:210353/150‑1 (MQ=255)
                    cccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGGAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAggg                                                                                                                       <  1:363999/150‑1 (MQ=255)
                     ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGTCGGAACAGGCCCAAAATGGa                                                                                                                      <  2:501401/150‑1 (MQ=255)
                     ccGCAGCAACGAGAATGTCCTGCGCTTAATCGTTGGACTGAAAATGCGTAAGGTCATCGTGGTACCGGGCAAGCGGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGa                                                                                                                      >  1:467226/1‑150 (MQ=255)
                         agcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGcaa                                                                                                                  >  2:554441/1‑150 (MQ=255)
                         agcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGcaa                                                                                                                  >  2:253616/1‑150 (MQ=255)
                               gagaATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACAt                                                                                                            >  1:875850/1‑150 (MQ=255)
                               gagaATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACAt                                                                                                            >  1:850061/1‑150 (MQ=255)
                                           cgcTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCaa                                                                                                <  1:253616/150‑1 (MQ=255)
                                            gcTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAAt                                                                                               <  2:191541/150‑1 (MQ=255)
                                                cATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGc                                                                                           <  1:686711/150‑1 (MQ=255)
                                                 aTCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCt                                                                                          <  2:466040/150‑1 (MQ=255)
                                                  tCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTg                                                                                         <  1:656333/150‑1 (MQ=255)
                                                        gCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATc                                                                                   <  2:635535/150‑1 (MQ=255)
                                                         ccTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCg                                                                                  >  2:94117/1‑150 (MQ=255)
                                                              ccATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTg                                                                             <  2:571636/150‑1 (MQ=255)
                                                               cATCCGCAAGGTCATCGTGGTACCAGGCAAGCTGGTCAACAGCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATCTGCTGGTAATCGGCCTgg                                                                            >  2:751696/1‑150 (MQ=255)
                                                                aTCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCAATCGA‑TGCGGGTGGGc                                                                                                                                         <  2:961512/88‑1 (MQ=38)
                                                                aTCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCAATCGA‑TGCGGGTGGGc                                                                                                                                         >  1:961512/1‑88 (MQ=38)
                                                                aTCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGc                                                                           >  1:709623/1‑150 (MQ=255)
                                                                 tCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCTCCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCg                                                                          <  1:565802/150‑1 (MQ=255)
                                                                    gCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAAGGCCCACCCGC‑ATAGATTGCGGGTGGGCGGCACAGCCCCAAAAGGGAGCACCAACCTGCGCAAACTCAATTTGATGGTAATCGGCCTGCCggac                                                                       >  2:8079/1‑148 (MQ=255)
                                                                    gCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTc                                                                       >  2:911451/1‑150 (MQ=255)
                                                                            aTCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCAATCGA‑TGCGGGTGGGCgtttt                                                                                                                                    <  2:617122/81‑5 (MQ=25)
                                                                            aTCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCAATCGA‑TGCGGGTGGGCgtttt                                                                                                                                    >  1:617122/1‑77 (MQ=25)
                                                                                   tACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACCAGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGc                                                        >  1:515051/1‑150 (MQ=255)
                                                                                       gggCAAGCTGGTCAACATCGTCGCAAAGTTTTTAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGtt                                                    >  1:203179/1‑150 (MQ=255)
                                                                                                 gTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTg                                                                                         <  2:740538/103‑1 (MQ=255)
                                                                                                 gTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTg                                                                                         >  1:740538/1‑103 (MQ=255)
                                                                                                 gTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgcg                                          >  1:185335/1‑150 (MQ=255)
                                                                                                  tCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgcgc                                         <  2:850061/150‑1 (MQ=255)
                                                                                                   cAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGcgcg                                        >  1:905202/1‑150 (MQ=255)
                                                                                                        tcgtcgCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCAcc                                   >  1:970847/1‑150 (MQ=255)
                                                                                                             gCCAACTGATGAAAACGCCCACCCAC‑TTGCCTGGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGgcgc                              >  1:869628/1‑150 (MQ=255)
                                                                                                                aaCTGATGAAAACGACCACCCGC‑ATCAATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCAc                           >  2:1031041/1‑150 (MQ=255)
                                                                                                                    gatgaAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgc                                           >  1:77247/1‑130 (MQ=255)
                                                                                                                    gatgaAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgc                                           <  2:77247/130‑1 (MQ=255)
                                                                                                                       gaAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTg                    >  2:66857/1‑150 (MQ=255)
                                                                                                                        aaaaCGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGa                   >  1:567518/1‑150 (MQ=255)
                                                                                                                        aaaaCGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGa                   >  1:945485/1‑150 (MQ=255)
                                                                                                                        aaaaCGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCAGTATGCCCGTGACATGATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGa                   <  2:869628/150‑1 (MQ=255)
                                                                                                                           aCGCCCACCCGC‑ATCGCTTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCg                <  2:567518/150‑1 (MQ=255)
                                                                                                                            cGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGt               <  2:875850/150‑1 (MQ=255)
                                                                                                                             gCCCACCCGCAATCGA‑TGCGGGTCGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGaacctg              >  2:550424/1‑145 (MQ=255)
                                                                                                                                     gC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAAt      <  2:467226/150‑1 (MQ=255)
                                                                                                                                          cGATTGCGGGTGGGCGGAACAGGAGTAAAAGGTAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGAc  <  1:279920/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGAC  >  NC_002947/5458806‑5459092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑