| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 684,849:1 | +A | 6.7% | coding (1401/2400 nt) | cadA‑II ← | cadmium translocating P‑type ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 684,849 | 1 | . | A | 6.7% | 228.7 / 10.4 | 60 | coding (1401/2400 nt) | cadA‑II | cadmium translocating P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base . (24/32); new base A (2/2); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01 | |||||||||||
GGCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAGCCAGCCACCA > NC_002947/684710‑684992 | ggCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGt < 1:639390/150‑1 (MQ=255) gCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTg > 1:686106/1‑150 (MQ=255) gTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGggcggc < 1:904625/150‑1 (MQ=255) cGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGa < 1:253281/150‑1 (MQ=255) cAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGTCCGCATGGGCACGCTCCAGGGCTTCGGATTCCTTGATC‑AGGAAGCCGTGGCGGGCGGCGAACCCGGTACCGGGCAt < 2:496531/150‑1 (MQ=255) cAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCAt < 1:378206/150‑1 (MQ=255) gcCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATc > 2:487277/1‑150 (MQ=255) gCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACGGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCgcggc < 2:208670/150‑1 (MQ=255) gCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCgcggc < 2:208931/150‑1 (MQ=255) tgtgAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑Agg > 1:638013/1‑104 (MQ=255) tgtgAACCACCTGCGGGCTGCCGGAGGTGAGGGTGACGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑Agg < 2:638013/104‑1 (MQ=255) tgAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCggcgggc > 1:459525/1‑150 (MQ=255) aaCCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCGCGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGt > 1:207861/1‑150 (MQ=255) cccaccTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTg < 2:1013323/149‑1 (MQ=255) ccTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCa < 2:213454/150‑1 (MQ=255) cTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAg < 1:352846/150‑1 (MQ=255) cTGCGGGCTGCAGGCGGTGGGGGTGCCGGGTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCGTGATC‑AGGATGCCGTGGCGGGCGGCGACCACGGTACCCGCCATGATCGCGGCGGGCGTGGCCac > 2:272060/1‑149 (MQ=255) tGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGAccc < 2:1075234/115‑1 (MQ=255) tGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGAccc > 1:1075234/1‑115 (MQ=255) cTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAgc < 1:487277/150‑2 (MQ=255) tGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAggg > 1:268046/1‑150 (MQ=255) ggAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTTGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGgcgaa > 1:538077/1‑148 (MQ=255) gAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAg < 2:246964/150‑1 (MQ=255) aGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAg < 2:908043/150‑1 (MQ=255) gggTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAgg < 1:1084144/150‑1 (MQ=255) gggTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAgg < 2:330944/150‑1 (MQ=255) ggTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGc < 1:73234/150‑1 (MQ=255) gCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGAt > 2:168455/1‑150 (MQ=255) ccGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGAcccc < 2:887148/92‑1 (MQ=255) ccGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGAcccc > 1:887148/1‑92 (MQ=255) gTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGAcc < 2:107859/150‑1 (MQ=255) tttGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAg < 1:933781/150‑1 (MQ=255) tCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacg > 1:361373/1‑150 (MQ=255) cgcgGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCCGCACGGCGACGGCGt > 1:763921/1‑150 (MQ=255) gcgGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCTGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGtt < 2:686106/150‑1 (MQ=255) ggTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGGGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGa > 1:541810/1‑150 (MQ=255) gTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGTCCATGACCAGCACGGCGACGGCGTTGAt < 1:869572/150‑1 (MQ=255) ttGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCgg > 1:885263/1‑76 (MQ=255) ttGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCgg < 2:885263/76‑1 (MQ=255) ccGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGGTCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAgcg > 2:1103340/1‑150 (MQ=255) cATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGTATGCCGTGGCGGGCGGCGACCCCGGTACCCGCCCTGATGGCGGCGGGCGTGGGCAGGCCGAGGGAGCAGGGGCAGGCGATGACCAGCACGGCGCCGGCCTTGATCAGCGCgg > 1:719918/1‑150 (MQ=255) cATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCgg > 2:255146/1‑150 (MQ=255) gggggACGCGTCAGGGCGTCGGCAGCCTTGAGC‑AGGATGCCGTGGGGGGCGGCGCGCCCGGTACCGGGCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCCGCGCGGtt < 2:507611/149‑1 (MQ=255) ggCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTc < 1:972707/150‑1 (MQ=255) gCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTcc < 2:252978/150‑1 (MQ=255) gCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCgg > 1:707877/1‑141 (MQ=255) gCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCgg < 2:707877/141‑1 (MQ=255) cAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTAc < 1:594821/150‑1 (MQ=255) aGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTAcc > 2:537898/1‑150 (MQ=255) gCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGc < 1:1026493/98‑1 (MQ=255) gCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGc > 2:1026493/1‑98 (MQ=255) ccacGGCATCC‑TGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcga > 1:559893/4‑114 (MQ=255) ccacGGCATCC‑TGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCacggcga < 2:559893/111‑1 (MQ=255) cTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCccag > 1:835251/1‑150 (MQ=255) ttCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCccagc > 2:816251/1‑150 (MQ=255) ggCATCC‑TGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGACCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGtt < 2:940725/130‑1 (MQ=255) ggCATCC‑TGATCAAGGATGCCGTGGCGGGCGGCGACCCCGGTACCGACCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGtt > 1:940725/1‑130 (MQ=255) aTCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAgccagcca < 1:537898/150‑1 (MQ=255) tCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAGccagccac < 1:816251/150‑1 (MQ=255) cTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTATCCAGCGGTATCCCAGtcagactcca > 2:1085994/1‑150 (MQ=255) | GGCGGTAAAGGTCGGCGCTGTTGCCGTCCAGCGCCTGGCTGTGAACCACCTGCGGGCTGCCGGAGGTGAGGGTGCCGGTTTTGTCGAACACTACGCGGTTGACCGCATGGGCACGCTCCAGGGCTTCGGCATCCTTGATC‑AGGATGCCGTGGCGGGCGGCGACCCCGGTACCGGCCATGATCGCGGCGGGCGTGGCCAGGCCGAGGGCGCAGGGGCAGGCGATGACCAGCACGGCGACGGCGTTGATCAGCGCGGTTTCCAGCGGTACCCCAGCCAGCCACCA > NC_002947/684710‑684992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |