| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,720,805:1 | +T | 8.2% | intergenic (+16/+34) | PP_2380 → / ← PP_2381 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,720,805 | 1 | . | T | 8.2% | 184.5 / 11.2 | 49 | intergenic (+16/+34) | PP_2380/PP_2381 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (24/21); new base T (2/2); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TCTGACCCTGACCATCGAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGCCGGCGGCG > NC_002947/2720669‑2720953 | tctGACCCTGACCATCGAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAgcggccct < 2:953653‑M2/150‑9 (MQ=255) tgaccctgaccATCGAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGCtgaacgcggcccttt < 2:930660‑M1/150‑16 (MQ=255) cctgaccATCGAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGTCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGAGGCCcttttctt > 2:62403/1‑150 (MQ=255) ctgaccATCGAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCcgtttcttt > 1:622484/1‑150 (MQ=255) cGAGGATGCCAAGCGCGCCAAGGAAACCCTGGAAGTTCAGGTACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTgc < 1:650073/150‑1 (MQ=255) gAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTgcg > 2:351740/1‑150 (MQ=255) ggATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGcc < 1:117798/150‑1 (MQ=255) cAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAg > 1:598953/1‑150 (MQ=255) cAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAg < 1:134131/150‑1 (MQ=255) aaGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGCTGAACcggccctttgatctgtcagtggttcat < 2:999894‑M2/136‑28 (MQ=255) aaGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGCTGAACcggccctttgatctgtcagtggttcat > 1:999894‑M2/1‑109 (MQ=255) cccTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAg < 2:598953/150‑1 (MQ=255) ccTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGc > 2:1047949/1‑150 (MQ=255) cTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCg > 2:776891/1‑150 (MQ=255) cTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCg < 1:776891/150‑1 (MQ=255) gTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGt > 1:843120/1‑150 (MQ=255) ggCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAgg < 1:953653/150‑1 (MQ=255) acaAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTc > 2:241273/1‑150 (MQ=255) acaAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTc > 2:1059015/1‑150 (MQ=255) gcTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAgg < 1:474092/150‑1 (MQ=255) tCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAg > 2:816727/1‑132 (MQ=255) tCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAg < 1:816727/132‑1 (MQ=255) tCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGt > 2:659669/1‑150 (MQ=255) aCGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCtt > 2:519014/1‑150 (MQ=255) aCGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCtt > 1:706370/1‑150 (MQ=255) ccGACGTGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCCGGGTCTTGCg > 2:656949/1‑150 (MQ=255) tggtggGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGCTGAACcggccctttgatct < 2:638961‑M2/70‑15 (MQ=255) tggtggGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGCTGAACcggccctttgatct > 1:638961‑M2/1‑56 (MQ=255) cATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCgg > 2:1020525/1‑150 (MQ=255) catgcacatgAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGt < 2:706370/150‑1 (MQ=255) catgcacatgAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGt < 2:745284/150‑1 (MQ=255) catgAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCcttttctttt > 1:211132/1‑50 (MQ=255) catgAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCcttttctttt < 2:211132/50‑1 (MQ=255) atgAACCACTGACAGATCAAAGGGCCGCgttcagcggccctttgatctgtcagtggttcatgtgcatgtgatcatgcccaccacccgcgtcggcgttgagcgcacgcacttgtgcctgaacttccagggtttcctt > 1:999894‑M1/1‑28 (MQ=255) atgAACCACTGACAGATCAAAGGGCCGCgttcagcggccctttgatctgtcagtggttcatgtgcatgtgatcatgcccaccacccgcgtcggcgttgagcgcacgcacttgtgcctgaacttccagggtttcctt < 2:999894‑M1/136‑109 (MQ=255) cACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAg > 2:765831/1‑150 (MQ=255) aCAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCgg > 1:798280/1‑150 (MQ=255) cAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTATGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGa < 1:351740/150‑1 (MQ=255) aGATCAAAGGGCCGCgttcagcggccctttgatctgtcagtggttcatgtgcatgtgatcatgcccacca > 1:638961‑M1/1‑15 (MQ=255) aGATCAAAGGGCCGCgttcagcggccctttgatctgtcagtggttcatgtgcatgtgatcatgcccacca < 2:638961‑M1/70‑56 (MQ=255) aGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTgg < 2:484130/112‑1 (MQ=255) aGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTgg > 1:484130/1‑112 (MQ=255) aTCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAg < 1:888207/79‑1 (MQ=255) aTCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAg > 2:888207/1‑79 (MQ=255) aTCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCt > 2:436803/1‑150 (MQ=255) aaaGGGCCGC‑GTTCAgcggccctttgatctgtcagtggttcatgtgcatgtgatcatgcccaccacccgcgtcggcgttgagcgcacgcacttgtgcctgaacttccagggtttccttggcgcccttggcatcctcgatggtcagggtca < 2:930660‑M2/150‑136 (MQ=255) aaaGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGACCCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGc > 1:628062/1‑150 (MQ=255) aaaGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGc > 1:329363/1‑150 (MQ=255) aaaGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGc < 1:659669/150‑1 (MQ=255) aaaGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGc > 1:420583/1‑150 (MQ=255) aGGGCCGCtgaacgcggccctttgatctgtcagtggttcatgtgcatgtgatcatgcccaccacccgcgtcggcgttgagcgcacgcacttgtgcctgaacttccagggtttccttggcgcccttggcatcctcgatggtcagggtcaga < 2:953653‑M1/150‑143 (MQ=255) gc‑gTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGCcggcggcg < 2:954171/150‑1 (MQ=255) | TCTGACCCTGACCATCGAGGATGCCAAGGGCGCCAAGGAAACCCTGGAAGTTCAGGCACAAGTGCGTGCGCTCAACGCCGACGCGGGTGGTGGGCATGATCACATGCACATGAACCACTGACAGATCAAAGGGCCGC‑GTTCAGCGGCCCTTTTCTTTTCAACTGCGCCTTTCAGCTACGAAAGCGCGGCAGCGGTCTGTCGAGGGTCAGCGACGTCAGGGTCTTGCGAACCTGGGCCTCATCGGCCTCCAGTGCCTTCAGGCTAGCGCGGATCTTGCCGGCGGCG > NC_002947/2720669‑2720953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |