| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,991,565:1 | +A | 11.8% | intergenic (‑43/+32) | vgrG‑I ← / ← hcpC‑I | type VI secretion system protein/hemolysin‑coregulated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,991,565 | 1 | . | A | 11.8% | 137.8 / 20.4 | 51 | intergenic (‑43/+32) | vgrG‑I/hcpC‑I | type VI secretion system protein/hemolysin‑coregulated protein |
| Reads supporting (aligned to +/- strand): ref base . (24/21); new base A (3/3); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
AAGGGTGCGCTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCATTGTCT > NC_002947/2991419‑2991714 | aaGGGTGCGCTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCcgggc‑ggg > 1:653603/1‑150 (MQ=32)aaGGGTGCGCTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCcgggc‑ggg > 1:879013/1‑150 (MQ=32) gcTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCt > 2:408122/1‑150 (MQ=32) gcTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCt > 1:860102/1‑150 (MQ=32) cTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACCCAAAGCCCGGGC‑GGGCATGGCCTG < 2:973523‑M1/150‑1 (MQ=255) gCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GG < 2:1114954‑M1/150‑1 (MQ=255) cccTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGG < 1:1113313‑M1/150‑1 (MQ=255) ccTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGACTCCAGCTAGACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑gggg > 2:820012/1‑150 (MQ=11) ttCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGcaca > 1:908264/1‑150 (MQ=33) gAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGt < 1:768780/150‑1 (MQ=35) cacTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCAGGCCAT‑GCCCGCCC‑GGGctttgtgttgattcagacggcgg > 1:694122‑M1/1‑127 (MQ=255) cacTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGgcggcg > 2:424926/1‑150 (MQ=35) acTTCGAATGCAGCTTTGCCAACGCGTGCCTCTAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCAGGCCAT‑GCCCGCCC‑GGGctttgtgttgattcagacggcgga < 2:694122‑M1/150‑25 (MQ=255) acTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGcggcgg < 2:987943/150‑1 (MQ=35) acTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGcggcgg < 1:180885/150‑1 (MQ=35) acTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGcggcgg < 1:270425/150‑1 (MQ=35) ttCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGt < 1:737137/150‑1 (MQ=35) cAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCa < 1:334265/150‑1 (MQ=32) gCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAgt > 1:668652/1‑150 (MQ=32) cTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAgtc > 2:441338/1‑150 (MQ=32) cTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAgtc > 2:969820/1‑150 (MQ=32) tttGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAgtcg > 1:1109657/1‑150 (MQ=32) cAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCAGGCCAT‑GCCCGCCC‑GGGcttt < 1:9099‑M1/111‑5 (MQ=255) cAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCAGGCCAT‑GCCCGCCC‑GGGcttt > 2:9099‑M1/1‑107 (MQ=255) gTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAgtc > 2:363710/1‑138 (MQ=33) gTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAgtc < 1:363710/138‑1 (MQ=33) gCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCgg < 2:668652/150‑1 (MQ=32) ccTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGgt < 1:441338/150‑1 (MQ=32) tCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCAGGCCAT‑GCCCGCCC‑GGGctttgtgttgat < 1:793880‑M1/108‑13 (MQ=255) tCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGCAGGCCAT‑GCCCGCCC‑GGGctttgtgttgat > 2:793880‑M1/1‑96 (MQ=255) tCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGcggcgg > 1:736949/1‑120 (MQ=33) tCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGcggcgg < 2:736949/120‑1 (MQ=33) tCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGgtgt > 1:394590/1‑150 (MQ=34) tCAAAGCTGTAGCGCAAATCAGATTGGCGAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGgtgt > 1:1051664/1‑150 (MQ=21) aaaGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTg > 2:1073880/1‑150 (MQ=35) aaaGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTg < 2:908264/150‑1 (MQ=35) gcgcAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCg > 1:875405/1‑150 (MQ=35) gCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACAt > 1:479406/1‑133 (MQ=35) gCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATc > 2:817888/1‑150 (MQ=35) tGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCT‑CCTCGCGGTGCCCACGTGATCTTTCGATACGCCAGCGATa < 1:1112577/150‑1 (MQ=11) cgccgTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTc > 2:498428/1‑150 (MQ=35) gccgTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCa > 2:988955/1‑150 (MQ=35) aaTCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTg > 2:687764/1‑150 (MQ=38) aTCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCCGGGGcatttgtgttgattcagacggcggacaaggatcttttatgcctagccaatc < 2:520993‑M1/89‑52 (MQ=255) aTCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCCGGGGcatttgtgttgattcagacggcggacaaggatcttttatgcctagccaatc > 1:520993‑M1/1‑38 (MQ=255) aTCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCATATCCTTCGGTGTTGGTTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTgg < 2:879013/150‑1 (MQ=255) aTCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTgg < 1:498428/150‑1 (MQ=38) cacaAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGNTCAGNCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTAGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAAACAGATAGgtgga > 1:51259/1‑148 (MQ=11) aaGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGGGTAGttt > 1:414921/1‑150 (MQ=37) gCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTc < 1:408122/150‑1 (MQ=38) ccGGGC‑GGGCATGGCCTGCCC‑GGGctttgtgttgattcagacggcggacaaggatcttttatgcctagccaatctgatttgcgctacagctttgaggcactcgttggcaaagctgaattcgaagtggtgtcgttcgaactccgcg > 2:740317‑M1/1‑24 (MQ=255) ccGGGC‑GGGCATGGCCTGCCC‑GGGctttgtgttgattcagacggcggacaaggatcttttatgcctagccaatctgatttgcgctacagctttgaggcactcgttggcaaagctgaattcgaagtggtgtcgttcgaactccgcg < 1:740317‑M1/145‑122 (MQ=255) ccGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCAt < 1:653152/150‑1 (MQ=38) gggc‑gggcATGGCCTGCCC‑GGGctttgtgttgattcagacggcggacaaggatcttttatgcctagccaatctgatttgcgctacagctttg > 2:176614‑M1/1‑22 (MQ=255) gggc‑gggcATGGCCTGCCC‑GGGctttgtgttgattcagacggcggacaaggatcttttatgcctagccaatctgatttgcgctacagctttg < 1:176614‑M1/92‑71 (MQ=255) ggc‑gggcATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCATTGt < 2:1109657/150‑1 (MQ=39) c‑gggcATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCATTGTCt < 1:1073880/150‑1 (MQ=38) | AAGGGTGCGCTGATGCCCTCGCGGAGTTCGAACGACACCACTTCGAATGCAGCTTTGCCAACGAGTGCCTCAAAGCTGTAGCGCAAATCAGATTGGCTAGGCATAAAAGATCCTTGTCCGCCGTCTGAATCAACACAAAGCCCGGGC‑GGGCATGGCCTGCCC‑GGGGCATTTGCACAGGTCAGGCGGCGGGTGCGCGCCAGTCGTCAGAGCCTTCGGTGTTGGCTACATCG‑TGAGCCCACGTGATCTTTCGATACGCCAGCGATACTTCAACCAGTTGGGTGTAGTTTTCATTGTCT > NC_002947/2991419‑2991714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |