Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 709,204 T→G 9.9% intergenic (+64/‑55) murJ → / → ribF lipid II flippase/bifunctional riboflavin kinase/FMN adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947709,2040TG 9.9% 121.1 / 4.3 47intergenic (+64/‑55)murJ/ribFlipid II flippase/bifunctional riboflavin kinase/FMN adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base G (2/3);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGACGGGGTTCACCGCGGCCACC  >  NC_002947/709055‑709352
                                                                                                                                                     |                                                                                                                                                    
tCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGt                                                                                                                                                      <  1:801711/150‑1 (MQ=255)
         gcgTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGGTGACAGGCAGCAgcgc                                                                                                                                             <  1:450810/150‑1 (MQ=255)
                        ttGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTtata                                                                                                                              >  1:200417/1‑150 (MQ=255)
                           gCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATc                                                                                                                           <  2:492168/150‑1 (MQ=255)
                           gCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATc                                                                                                                           <  2:232188/150‑1 (MQ=255)
                           gCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATc                                                                                                                           <  2:232075/150‑1 (MQ=255)
                            cTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCg                                                                                                                          <  2:258548/150‑1 (MQ=255)
                            cTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCg                                                                                                                          >  2:1104026/1‑150 (MQ=255)
                             tGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTtata                                                                                                                              <  1:1023999/145‑1 (MQ=255)
                             tGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTtata                                                                                                                              >  2:1023999/1‑145 (MQ=255)
                                      cTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTAt                                                                                                                >  2:686717/1‑150 (MQ=255)
                                      cTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTAt                                                                                                                <  1:464480/150‑1 (MQ=255)
                                       tgtgtgGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATg                                                                                                               >  2:1075888/1‑150 (MQ=255)
                                         tgtgGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCACAGCACACGTCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAg                                                                                                             >  1:852044/1‑150 (MQ=255)
                                            ggCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCaa                                                                                                          >  1:909244/1‑150 (MQ=255)
                                              cTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTCTAATCGGCCACTTTATGAGCaaga                                                                                                        >  2:119031/1‑150 (MQ=255)
                                               ttCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCaagaa                                                                                                       >  1:554778/1‑150 (MQ=255)
                                                    gCCCCGGCCTTTCGCGCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTCTTTGAGCGTCTCCGCCCTTGGGCGCTGCTGCTTGTCAAAAGCGCGGAGGTGGGGGTATAATCGGCCACTTTATGAGCAAGAAGcgcg                                                                                                  <  1:810114/150‑1 (MQ=255)
                                                      cccGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGtt                                                                                                >  1:434773/1‑150 (MQ=255)
                                                      cccGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGtt                                                                                                <  1:297641/150‑1 (MQ=255)
                                                            aTTTCGCTCGCGTTGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGGTTTTTGCATTCCACGCCGCCCTTGGGCGCTGGTGCTTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAg                                                                                          <  1:498623/150‑1 (MQ=255)
                                                                cgctcgcAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTgg                                                                                      <  1:555494/150‑1 (MQ=255)
                                                                   tcgcAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTc                                                                                   >  1:541470/1‑150 (MQ=255)
                                                                      cAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAg                                                                                >  1:934861/1‑150 (MQ=255)
                                                                         ggCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTc                                                                             >  1:150544/1‑150 (MQ=255)
                                                                           ccTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCtt                                                                           <  2:434773/150‑1 (MQ=255)
                                                                             ttGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTca                                                                         <  2:957838/150‑1 (MQ=255)
                                                                             ttGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTca                                                                         <  2:540494/150‑1 (MQ=255)
                                                                                 aCTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAAc                                                                     <  2:909244/150‑1 (MQ=255)
                                                                                       gCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTgcgc                                                               >  1:239751/1‑150 (MQ=255)
                                                                                         ttttGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGccc                                                             >  1:1110823/1‑150 (MQ=255)
                                                                                                         gTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCtgt                                             <  2:92095/150‑1 (MQ=255)
                                                                                                            ggTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGc                                                                                                            >  2:149419/1‑84 (MQ=255)
                                                                                                            ggTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGc                                                                                                            <  1:149419/84‑1 (MQ=255)
                                                                                                            ggTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTc                                          >  1:373072/1‑150 (MQ=255)
                                                                                                                 ttCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGccac                                     <  1:372655/150‑1 (MQ=255)
                                                                                                                      gattataaccacacccGGGCGCTGGTGACAGGCAGCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGa                                                                                 >  2:145953‑M1/17‑101 (MQ=255)
                                                                                                                      gattataaccacacccGGGCGCTGGTGACAGGCAGCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGa                                                                                 <  1:145953‑M1/85‑1 (MQ=255)
                                                                                                                             accacacccGGGCGCTGGTGACAGGCAGCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGc                                        >  1:87377‑M1/10‑135 (MQ=255)
                                                                                                                             accacacccGGGCGCTGGTGACAGGCAGCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGc                                        <  2:87377‑M1/126‑1 (MQ=255)
                                                                                                                                gccCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGa                      <  1:1075888/150‑1 (MQ=255)
                                                                                                                                 cccTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGAc                     >  2:144588/1‑150 (MQ=255)
                                                                                                                                      GGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCAtt                                 <  2:589084‑M1/133‑1 (MQ=255)
                                                                                                                                      GGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCAtt                                 >  1:589084‑M1/1‑133 (MQ=255)
                                                                                                                                        GCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCgacgggggtg              <  1:1084579‑M1/150‑4 (MQ=255)
                                                                                                                                        GCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGACGGGGTTc              <  1:34817‑M1/150‑1 (MQ=255)
                                                                                                                                                gcCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGACGGGGTTCACCGCGGc      >  1:363593/1‑150 (MQ=255)
                                                                                                                                                    gTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGACGGGGTTCACCGCGGccacc  >  1:117206/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCCTGGCGGGCGTCGTGACGTATTTTGGCTGCCTGTACCTGTGTGGCTTCCGGCCCCGGCATTTCGCTCGCAAGGCCTTGCACTGAGGCTTTTGGCGGGTCAGGCGTCGGTTTTTCGCATTCCACGCCGCCCTTGGGCGCTGCTGCCTGTCACCAGCGCCCGGGTGTGGTTATAATCGGCCACTTTATGAGCAAGAAGCGCGTTATGCAGCTGGTTCGAGGTCTTCACAACCTGCGCCCCGAGCACCGGGGCTGTGTCGCCACCATTGGCAACTTCGACGGGGTTCACCGCGGCCACC  >  NC_002947/709055‑709352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑