Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 937,766 A→T 8.3% L2620Q (CTG→CAG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947937,7660AT 8.3% 143.5 / 4.4 48L2620Q (CTG→CAG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (21/23);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

CCGCTGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCCGACGACAATG  >  NC_002947/937625‑937915
                                                                                                                                             |                                                                                                                                                     
ccGCTGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGcc                                                                                                                                               >  1:837683/1‑150 (MQ=255)
    tGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCTGCGCTGCCGggt                                                                                                                                           <  1:939229/150‑1 (MQ=255)
          tgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGcc                                                                                                                                               <  2:327061/140‑1 (MQ=255)
          tgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGcc                                                                                                                                               >  1:327061/1‑140 (MQ=255)
          tgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGggtcgaggt                                                                                                                                     <  1:964128/150‑1 (MQ=255)
          tgctgcCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGggtcgaggt                                                                                                                                     <  1:964062/150‑1 (MQ=255)
               ccATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATc                                                                                                                                <  1:598236/150‑1 (MQ=255)
                cATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCa                                                                                                                               <  2:458239/150‑1 (MQ=255)
                   cGGCAGTGACATGCAGTTCGCTCGGCCTGGTCGTCGTCACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCCCGGGTCATTCTGTAGCAGGGTCTGCGGATCACCCGGCAGTGTAGCCGGGGCAGGGTCGATCAgca                                                                                                                            <  2:393011/150‑3 (MQ=255)
                     gCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGaaa                                                                                                                          <  1:1088275/150‑1 (MQ=255)
                     ccaccgacgcACAGTTCGTCCGTGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGaaa                                                                                                                          <  2:905319/146‑1 (MQ=255)
                      cAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAg                                                                                                                         <  1:463588/150‑1 (MQ=255)
                                cAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTcgc                                                                                                               >  1:62232/1‑150 (MQ=255)
                                cAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTcgc                                                                                                               >  1:704301/1‑150 (MQ=255)
                                 aGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTcgcg                                                                                                              <  2:792459/150‑1 (MQ=255)
                                     cGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCa                                                                                                          <  2:412493/150‑1 (MQ=255)
                                           ggCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGc                                                                                                    <  1:814782/150‑1 (MQ=255)
                                              tGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCgg                                                                                                 <  1:1039167/150‑1 (MQ=255)
                                                     cGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGAc                                                                                          <  2:802029/150‑1 (MQ=255)
                                                         accaccAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGc                                                                                      <  1:205168/150‑1 (MQ=255)
                                                           caccaGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGt                                                                                    >  1:964165/1‑150 (MQ=255)
                                                             ccaGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGc                                                                                  <  1:638633/150‑1 (MQ=255)
                                                                   gCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTgg                                                                            >  1:1019249/1‑150 (MQ=255)
                                                                                   cggttCAGACGCGTTGCCGGGCGGTTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGAc                                                            <  2:62232/147‑1 (MQ=255)
                                                                                                  gCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTc                                             >  1:755626/1‑150 (MQ=255)
                                                                                                           gtcggtcTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGAcc                                    >  2:136190/1‑150 (MQ=255)
                                                                                                                  tGTCCCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                             <  1:169377/150‑1 (MQ=255)
                                                                                                                  tGTACCAGGCTCAGGCGTTCACCCGTCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                             <  2:554875/150‑1 (MQ=255)
                                                                                                                  tGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                             >  2:626011/1‑150 (MQ=255)
                                                                                                                      ccAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTa                                                                                                                       <  2:1069433/56‑1 (MQ=255)
                                                                                                                      ccAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTa                                                                                                                       >  1:1069433/1‑56 (MQ=255)
                                                                                                                      ccAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGCACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCa                         >  2:95744/1‑150 (MQ=255)
                                                                                                                       cAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAg                        >  1:234564/1‑150 (MQ=255)
                                                                                                                          gCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGcc                     <  1:76495/150‑1 (MQ=255)
                                                                                                                           cTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCg                    >  1:1031968/1‑150 (MQ=255)
                                                                                                                           cTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCg                    >  1:1032026/1‑150 (MQ=255)
                                                                                                                              aGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCg                 >  2:600259/1‑150 (MQ=255)
                                                                                                                              aGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCg                 >  2:849385/1‑150 (MQ=255)
                                                                                                                                 cGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGgcg              <  1:626011/150‑1 (MQ=255)
                                                                                                                                   ttCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGcc            >  1:598641/1‑150 (MQ=255)
                                                                                                                                   tcgaCCCGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGcc            >  1:94142/4‑150 (MQ=255)
                                                                                                                                      aCCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCcgac         >  2:250276/1‑150 (MQ=255)
                                                                                                                                       cccGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGc                                                <  2:61471/110‑1 (MQ=255)
                                                                                                                                       cccGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGc                                                >  1:61471/1‑110 (MQ=255)
                                                                                                                                        ccGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                             >  2:444567/1‑128 (MQ=255)
                                                                                                                                        ccGGCTGCGCTGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAg                             <  1:444567/128‑1 (MQ=255)
                                                                                                                                        ccGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCcgacga       >  1:964108/1‑150 (MQ=255)
                                                                                                                                        ccGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCcgacga       >  2:493414/1‑150 (MQ=255)
                                                                                                                                            cagcgcagcCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGAGCCGTTACTGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCCGACGANAAt   <  2:704301/150‑1 (MQ=255)
                                                                                                                                             agcgcagcCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCCGACGACAAtg  >  1:114312/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
CCGCTGATGCTGCTGCCATCGGCAGCGACGACCAGTTCGCTCGGGCTGGTCGGCGTGACCACCAGTGGCACGTCGTAGGTCAGCGCTTCAGAGGCGTTGCCGCTCGGGTCGGTCTGTACCAGGCTCAGGCGTTCACCCGGCAGCGCAGCCGGGTCGAGGTCGATCAGGAAAGTACCGTTCGCGGTCACTGTGCCGGTACCGACCAGCGTGCCATTGGCATCGCGCACCTCGACGGTAGCACCGGCTTCGCCGCGACCGCCCAAGGTCAGGCCGTCGGCGCCGACGACAATG  >  NC_002947/937625‑937915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑