| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 953,233 | C→T | 10.5% | H13H (CAC→CAT) ‡ | cyoC → | cytochrome bo terminal oxidase subunit III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 953,233 | 0 | C | T | 10.5% | 96.6 / 3.6 | 38 | H13H (CAC→CAT) ‡ | cyoC | cytochrome bo terminal oxidase subunit III |
| Reads supporting (aligned to +/- strand): ref base C (22/12); new base T (2/2); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
TTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGC > NC_002947/953085‑953371 | ttCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACg > 2:1080531/1‑150 (MQ=255) cGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg < 2:417911/150‑1 (MQ=255) agTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATga < 2:954559/150‑1 (MQ=255) cgcgcATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGacc < 2:309968/150‑1 (MQ=255) aTCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGAGGaccaccac > 2:400249/1‑150 (MQ=255) cGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATgag < 1:313628/133‑3 (MQ=255) cGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATgag > 2:313628/1‑131 (MQ=255) gAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCACGAGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACcaccaccac > 1:261863/1‑150 (MQ=255) gAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGAt > 1:747610/1‑150 (MQ=255) gcgcTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGAcc < 1:1080531/150‑1 (MQ=255) ccAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGCGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCAc > 1:324401/1‑98 (MQ=255) ccAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCAc < 2:324401/98‑1 (MQ=255) cAAGGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTggg > 2:1128244/1‑150 (MQ=255) gCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCCGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCCCCTCCACGACGCGGGCCAGATGACCGTACTGGGCTTc < 2:407572/150‑1 (MQ=255) gcctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCt > 1:614026/1‑150 (MQ=255) ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGaccac < 1:972409/101‑1 (MQ=255) ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGaccac > 2:972409/1‑101 (MQ=255) ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCtga < 1:152642/150‑1 (MQ=255) cgcACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGTGCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGa < 1:353043/150‑1 (MQ=255) gcACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCt < 1:708381/140‑1 (MQ=255) gcACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCt > 2:708381/1‑140 (MQ=255) aCGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGTGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTg > 2:195001/1‑150 (MQ=255) gAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTg > 2:671675/1‑150 (MQ=255) aaTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTgg < 1:440710/114‑1 (MQ=255) aaTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTgg > 2:440710/1‑114 (MQ=255) aaTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt > 1:1103793/1‑150 (MQ=255) aaTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt > 1:1103819/1‑150 (MQ=255) gTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGc > 1:444304/1‑150 (MQ=255) tttGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCg > 1:142185/1‑150 (MQ=255) ggTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCa < 2:471542/60‑1 (MQ=25) ggTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCa > 1:471542/1‑60 (MQ=21) tAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGcc > 1:616504/1‑150 (MQ=255) tCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTAACGGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCAcc > 1:560835/1‑150 (MQ=255) tCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTa < 2:66693/146‑1 (MQ=255) tCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTa > 1:66693/1‑146 (MQ=255) tCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGa > 2:501453/1‑60 (MQ=255) tCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGa < 1:501453/60‑1 (MQ=255) cAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTg > 2:621138/1‑150 (MQ=255) tGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCa > 2:664475/1‑150 (MQ=255) gctCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGGTGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCcggc > 1:194992/1‑150 (MQ=255) | TTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGC > NC_002947/953085‑953371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |