| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,155 | T→A | 16.7% | intergenic (+106/‑207) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,155 | 0 | T | A | 16.7% | 68.3 / 8.8 | 30 | intergenic (+106/‑207) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (11/14); new base A (3/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01 | |||||||||||
CCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATT > NC_002947/955010‑955297 | ccAGACCGGTGCGGATGTGCAGATGACCTCCGGGGGCGGAAACTGCTGCCTGTTTCGCGAGAGCCCCGGCCACGGTGCCGGGGTTTTGTTTTGGGGGTATTTTCCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACGGTTCC‑t < 2:336791/150‑1 (MQ=25) cTGCGGATGTGCTGATGACCTACGCCCGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGc < 1:443543/150‑1 (MQ=255) gCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg < 2:12887/150‑1 (MQ=255) cGGATGTGCTGATGACCTCCGCGCGCTGTTATTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCAGGGCTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCggg < 1:801564/150‑1 (MQ=38) tgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGaa < 1:699037/150‑1 (MQ=255) tACGCACGCTGCAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGc > 2:325644/1‑150 (MQ=255) acgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCgg > 1:807222/1‑127 (MQ=25) tttCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGCggg > 1:648522/1‑109 (MQ=21) tttCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGCggg < 2:648522/109‑1 (MQ=21) ttttATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGc > 2:508698/4‑63 (MQ=11) ttttATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGc < 1:508698/60‑1 (MQ=11) gtgCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc < 1:325644/150‑1 (MQ=255) ggCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAg > 1:517073/1‑42 (MQ=255) ggCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAg < 2:517073/42‑1 (MQ=255) tctTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATAc < 1:66810/84‑1 (MQ=255) tctTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATAc > 2:66810/1‑84 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTa > 2:506123/1‑138 (MQ=255) ttCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTa < 1:506123/138‑1 (MQ=255) ggTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa > 1:350184/1‑134 (MQ=255) ggTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACa < 2:350184/134‑1 (MQ=255) aCCCGCTCCTACAGGAACCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCt > 2:959814/1‑124 (MQ=25) aCCCGCTCCTACAGGAACCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCt < 1:959814/124‑1 (MQ=25) aCCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc > 2:1027001/1‑102 (MQ=255) aCCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc < 1:1027001/102‑1 (MQ=255) ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 2:952620/1‑150 (MQ=255) tACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt > 1:124554/1‑150 (MQ=255) gTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGaa > 2:371989/1‑101 (MQ=255) gTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGaa < 1:371989/101‑1 (MQ=255) gTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc > 2:576433/1‑139 (MQ=255) gTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc < 1:576433/139‑1 (MQ=255) | CCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATT > NC_002947/955010‑955297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |