Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 981,263 A→T 7.7% D46V (GAC→GTC)  hscA → DnaK‑like molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947981,2630AT 7.7% 152.0 / 3.8 52D46V (GAC→GTC) hscADnaK‑like molecular chaperone
Reads supporting (aligned to +/- strand):  ref base A (28/20);  new base T (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

AGATAAGCATGGCCCTACTGCAGATTGCCGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAGCGCCTGATGGGGCGCGGCC  >  NC_002947/981119‑981403
                                                                                                                                                |                                                                                                                                            
aGATAAGCATGGCCCTACTGCAGATTGCCGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACgcgc                                                                                                                                         >  2:583774/1‑150 (MQ=255)
   tAAGCATGGCCCTACTGCAGATTGCCGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAgg                                                                                                                                      <  1:583774/150‑1 (MQ=255)
        aTGGCCCTACTGCAGATTGCCGAACCCGGTCAAAGCTCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAAc                                                                                                                                 <  2:472169/150‑1 (MQ=255)
        aTGGCCCTACTGCAGATTGCCGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAAc                                                                                                                                 >  1:598526/1‑150 (MQ=255)
            cccTACTGCAGATTGCCGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCa                                                                                                                             >  1:668090/1‑150 (MQ=255)
                            cGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGt                                                                                                             >  1:204619/1‑150 (MQ=255)
                                 ccGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCt                                                                                                        >  1:294024/1‑150 (MQ=255)
                                   ggTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGAACTCGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCAAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTAc                                                                                                      >  1:1050338/1‑150 (MQ=255)
                                          gCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAg                                                                                               >  2:215762/1‑150 (MQ=255)
                                              tCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCGCGTCGGGCAggggc                                                                                                                                   >  2:973878/1‑108 (MQ=255)
                                              tCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCGCGTCGGGCAggggc                                                                                                                                   <  1:973878/110‑3 (MQ=255)
                                                 gCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCaa                                                                                        <  1:185378/150‑1 (MQ=255)
                                                    gCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTGCATCGGTGGGCGCAACgg                                                                                     >  2:757491/1‑149 (MQ=255)
                                                    gCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGa                                                                                     >  1:498524/1‑150 (MQ=255)
                                                    gCACCAGCGCCGCCTGGCGGGTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGAACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAAGGCGCAACGa                                                                                     >  2:337162/1‑150 (MQ=255)
                                                     cACCAGCGCCGCTGGGCGGTTGGAATCGACATGGGTACCACCAACTCAATGGTCGCTGCACTGAGCAGCGGCTGTAGCGAGCCACTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGaa                                                                                    <  2:36017/150‑1 (MQ=255)
                                                       ccAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGt                                                                                  <  1:650959/150‑1 (MQ=255)
                                                        cAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAgg                                                                                                                                      <  2:804076/97‑1 (MQ=255)
                                                        cAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAgg                                                                                                                                      >  1:804076/1‑97 (MQ=255)
                                                        cAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAg                                                                                               <  2:128220/136‑1 (MQ=255)
                                                        cAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAg                                                                                               >  1:128220/1‑136 (MQ=255)
                                                         aGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTgg                                                                                <  1:218386/150‑1 (MQ=255)
                                                                  tGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCt                                                                                                                         >  1:266219/1‑100 (MQ=255)
                                                                  tGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCt                                                                                                                         <  2:266219/100‑1 (MQ=255)
                                                                  tGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCcg                                                                       >  1:408388/1‑150 (MQ=255)
                                                                    gCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCcgcg                                                                     >  1:401533/1‑150 (MQ=255)
                                                                       gTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGt                                                                  >  2:177930/1‑150 (MQ=255)
                                                                        ttGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTg                                                                 <  1:399453/150‑1 (MQ=255)
                                                                         tGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGa                                                                <  2:401533/150‑1 (MQ=255)
                                                                          ggAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGAt                                                               <  2:598526/150‑1 (MQ=255)
                                                                           gAACCGACCTGGGTACTATCAGCTCAATAGCTGCTGCCCTGCGCAGCGTCCGTAGGGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCCGTGCGCTAGCTCGAAGGGCGCAACGAAGTGGGGCACGCCGCGCGTGAtg                                                              <  2:579981/150‑1 (MQ=255)
                                                                              tCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGAtgctg                                                           >  1:819503/1‑150 (MQ=255)
                                                                               cGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGAtgctgc                                                          <  2:1128886/150‑1 (MQ=255)
                                                                                     gggTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAg                                                    >  1:596431/1‑150 (MQ=255)
                                                                                      ggTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGc                                                   >  2:773017/1‑150 (MQ=255)
                                                                                            accaACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCg                                             >  1:189860/1‑150 (MQ=255)
                                                                                                 cTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTgggg                                                                              >  1:832726/1‑112 (MQ=255)
                                                                                                 cTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTgggg                                                                              <  2:832726/112‑1 (MQ=255)
                                                                                                       ggccGCTGCGCAGTGCAGCGACCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTgggg                                                                              <  2:968677/103‑1 (MQ=255)
                                                                                                        gTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTg                                 >  1:195626/1‑150 (MQ=255)
                                                                                                         tCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGc                                <  2:791450/150‑1 (MQ=255)
                                                                                                                     gcAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAgc                    <  2:949370/150‑1 (MQ=255)
                                                                                                                       aGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAgcgc                  >  2:18811/1‑150 (MQ=255)
                                                                                                                           gCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTg                                                                                 >  2:735682/1‑83 (MQ=255)
                                                                                                                           gCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTg                                                                                 <  1:735682/83‑1 (MQ=255)
                                                                                                                               tAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAGCGCCTGATggg          <  2:294024/150‑1 (MQ=255)
                                                                                                                                   gAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAGCGCCTGATGGGgcgc      >  2:311309/1‑150 (MQ=255)
                                                                                                                                   gAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAGCGCCTGATGGGgcgc      >  2:117551/1‑150 (MQ=255)
                                                                                                                                   gAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCCGGCAAGCGCCTGATGGGgcgc      >  1:335911/1‑150 (MQ=255)
                                                                                                                                       cccTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAGCGCCTGATGGGGCGCGGcc  >  2:649929/1‑150 (MQ=255)
                                                                                                                                        ccTGCGCGTCGGGCAGGGTAACTTCATTCTGCCGTCCGCGGTGCGCTACCTCGaa                                                                                                <  2:624310/55‑1 (MQ=255)
                                                                                                                                        ccTGCGCGTCGGGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGaa                                                                                                >  1:624310/1‑55 (MQ=37)
                                                                                                                                                |                                                                                                                                            
AGATAAGCATGGCCCTACTGCAGATTGCCGAACCCGGTCAAAGCCCTCAGCCGCACCAGCGCCGCCTGGCGGTTGGAATCGACCTGGGTACCACCAACTCAATGGTCGCTGCACTGCGCAGCGGCCGTAGCGAGCCCCTGCCCGACGCGCAGGGTAACGTCATTCTGCCGTCCGCGGTGCGCTACCTCGAAGGGCGCAACGAAGTGGGGCAGGCCGCGCGTGATGCTGCCTCCAGCGACCCGTTGAACACCGTGCTGTCGGTCAAGCGCCTGATGGGGCGCGGCC  >  NC_002947/981119‑981403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑