Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,096,745 G→C 7.6% G74G (GGC→GGG)  lptB ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,096,7450GC 7.6% 144.0 / 2.6 48G74G (GGC→GGG) lptBABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (18/26);  new base C (2/2);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCCGACGATCTGG  >  NC_002947/1096598‑1096880
                                                                                                                                                   |                                                                                                                                       
cTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGcc                                                                                                                                       >  2:267939/1‑150 (MQ=255)
             tcacgCTCTTTGAGTCGGCGGGGCTGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGGTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCtgg                                                                                                                          <  2:63813/146‑1 (MQ=255)
               cAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAACTTGCGGAAGAACGAGGCGGCCGGCGGCAGGGAGCCGAGTCCGGCACGGGCGCGGCCGTGCATGGGCtggtg                                                                                                                        >  2:678543/1‑150 (MQ=255)
                aGCTCTTTGCGGTGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCtggtgg                                                                                                                       <  1:319660/150‑1 (MQ=255)
                aGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCtggtgg                                                                                                                       <  1:1028094/150‑1 (MQ=255)
                aGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCtggtgg                                                                                                                       >  1:136553/1‑150 (MQ=255)
                 gCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGc                                                                                                                      >  2:51394/1‑150 (MQ=255)
                   tctTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGATAGTTGATTCCGGCACGGGCGCGGATGTTTTTTGGCTGGTGGCTg                                                                                                                    <  1:38326/150‑1 (MQ=255)
                     tttGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGGAGATCGAGGCTTCCTGCGACAGGTAGCCGATTGCGGCACGGGGGGGGGAGTGCATGGGCGGGTGGCTGag                                                                                                                  >  2:516195/1‑149 (MQ=255)
                          ggcggcCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGa                                                                                                             <  2:136553/150‑1 (MQ=255)
                           gcggcCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAg                                                                                                            <  1:1044749/150‑1 (MQ=255)
                           gcggcCGTCCCGATCGAGGTCCCTGTGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGTATGGGCTGGTGGCTGACATCGAg                                                                                                            <  2:556173/150‑1 (MQ=255)
                                cGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCAAGGCTTCCTGCGGCAGGTAGCCGATTACGGCATGGGCGCGGCCGTGCATGGGCTGGTGGCTGAAATCGAGGTTGt                                                                                                       >  1:73178/1‑150 (MQ=255)
                                 gTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCGATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTc                                                                                                      <  2:143372/150‑1 (MQ=255)
                                    ccGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGAt                                                                                                   <  1:1029940/150‑1 (MQ=255)
                                    ccGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGAt                                                                                                   <  1:267939/150‑1 (MQ=255)
                                    ccGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGAt                                                                                                   <  2:714836/150‑1 (MQ=255)
                                        tCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGt                                                                                               >  2:531549/1‑150 (MQ=255)
                                               ccTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCATGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGc                                                                                        <  2:650921/150‑1 (MQ=255)
                                                      ggTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTc                                                                                 >  1:359893/1‑150 (MQ=255)
                                                         ctcagggATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGc                                                                              <  1:260502/145‑1 (MQ=255)
                                                              ggATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGa                                                                         <  1:531549/150‑1 (MQ=255)
                                                                    ccATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGcc                                                                   <  2:1094932/150‑1 (MQ=255)
                                                                              gtCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCAt                                                         <  2:379160/150‑1 (MQ=255)
                                                                              gtCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCAt                                                         <  2:359893/150‑1 (MQ=255)
                                                                               tCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATg                                                        >  1:271729/1‑150 (MQ=255)
                                                                                cGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGt                                                       <  1:680113/150‑1 (MQ=255)
                                                                                    gaccgacAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGaa                                                   >  1:950116/1‑150 (MQ=255)
                                                                                      ccgacAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGc                                                                    >  2:1078481/1‑131 (MQ=255)
                                                                                      ccgacAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGc                                                                    <  1:1078481/131‑1 (MQ=255)
                                                                                                gCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCtt                                       <  1:678543/150‑1 (MQ=255)
                                                                                                  ggAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGc                                                                                                                      <  2:1017348/69‑1 (MQ=255)
                                                                                                  ggAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGc                                                                                                                      >  1:1017348/1‑69 (MQ=255)
                                                                                                   gAAGATCGAGGCTTCCTGCGGCAGGTGGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGcc                                    >  1:150105/1‑150 (MQ=255)
                                                                                                   gAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTggc                                    >  1:112857/1‑148 (MQ=255)
                                                                                                        tCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGaa                                                   <  1:186478/130‑1 (MQ=255)
                                                                                                        tCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGaa                                                   >  2:186478/1‑130 (MQ=255)
                                                                                                        tCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGgcgc                               <  2:930546/150‑1 (MQ=255)
                                                                                                            ggCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTAACCGGCCCTGGTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGtt                           <  2:427512/150‑1 (MQ=255)
                                                                                                                tCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTgggg                       <  1:876367/150‑1 (MQ=255)
                                                                                                                              gCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCcgac         <  2:326817/150‑1 (MQ=255)
                                                                                                                              gCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCcgac         >  1:719391/1‑150 (MQ=255)
                                                                                                                                 gagtCCGGCACGGGCGCGCCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCCGACGAt      <  2:252376/147‑1 (MQ=255)
                                                                                                                                 gATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTTGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCGTGCCGGCGCCGTTGGGGCCGAGCAGGCCGACGAt      >  1:72802/1‑150 (MQ=255)
                                                                                                                                 gATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCCGACGAt      >  2:1010538/1‑150 (MQ=255)
                                                                                                                                     catgCACGGCCGCGCCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCCGACGATCTgg  >  2:210976/4‑150 (MQ=255)
                                                                                                                                       tgCACGGCCGCGCCCGTGC‑CGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCg                                                                                >  2:399430/2‑69 (MQ=25)
                                                                                                                                       tgCACGGCCGCGCCCGTGC‑CGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCg                                                                                <  1:399430/68‑1 (MQ=25)
                                                                                                                                                   |                                                                                                                                       
CTGCAGCAGGCTTTCCAGCTCTTTGCGGCGGCCGTCCCGATCGAGGTCCTTGCGGGTCTCGAGGATGGCCATGATGTTGTCGGCGACCGACAGCTTGCGGAAGATCGAGGCTTCCTGCGGCAGGTAGCCGATTCCGGCACGGGCGCGGCCGTGCATGGGCTGGTGGCTGACATCGAGGTTGTCGATCAGTACCCGGCCCTGTTCGGCCTGGACCAGCCCGACAATCATGTAGAAGCAAGTGGTCTTGCCGGCGCCGTTGGGGCCGAGCAGGCCGACGATCTGG  >  NC_002947/1096598‑1096880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑