| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,184,611 | C→T | 13.2% | intergenic (‑221/‑191) | mltF ← / → purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,184,611 | 0 | C | T | 13.2% | 90.0 / 5.8 | 38 | intergenic (‑221/‑191) | mltF/purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (3/2); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
TGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATT > NC_002947/1184464‑1184749 | tgGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg < 2:745632/150‑1 (MQ=255) ggggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATTTCACAGGCGCAGAAtgt > 2:889838/1‑150 (MQ=255) ggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATgtgt < 1:637213/150‑1 (MQ=255) gATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGa < 1:696599/150‑1 (MQ=255) aTCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg < 1:1020100/128‑1 (MQ=255) aTCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg > 2:1020100/1‑128 (MQ=255) aTCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGa > 2:747979/1‑150 (MQ=255) aTCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGa > 2:162893/1‑150 (MQ=255) aTCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGa > 1:963077/1‑150 (MQ=255) ggCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCt < 1:926854/150‑1 (MQ=255) ccAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑gcggc > 1:1107734/1‑150 (MQ=255) ggCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGa < 2:1107734/150‑1 (MQ=255) gCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGAt < 2:963077/150‑1 (MQ=255) gccgccAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACAGCG‑TCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcccc > 1:345353/1‑150 (MQ=255) cgccAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGa > 2:739857/1‑150 (MQ=255) gccAGGTGTCCGCCGTAAGATATTCAGCGCCTAGGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCNGaa < 2:696852/150‑1 (MQ=255) gtgtCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGCTCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGCAGTCCg > 1:450708/1‑150 (MQ=255) gtgtCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCg > 1:391532/1‑150 (MQ=255) tgtCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCgg < 1:889838/150‑1 (MQ=255) tCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGAt > 2:217982/1‑103 (MQ=255) tCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGAt < 1:217982/103‑1 (MQ=255) cGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTcc > 1:589453/1‑150 (MQ=255) ccTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTccgc > 1:554881/1‑150 (MQ=255) aGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCcgccgc < 1:439498/148‑1 (MQ=255) aGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCcgccgc > 2:439498/1‑148 (MQ=255) atatTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACCCCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCgg < 1:162893/150‑1 (MQ=255) tgcgcCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACTT‑TCTGCGCTCGATCTCGTCTACAGCGGGTCATCTTTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCg > 1:806152/2‑150 (MQ=21) cccTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGACGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTgg < 1:270216/149‑1 (MQ=255) ctgtGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCt > 2:128375/4‑150 (MQ=37) gagaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCTTTTGTCTATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTaga < 1:747979/150‑1 (MQ=38) aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCa > 2:177373/1‑150 (MQ=255) gAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAgg < 2:1024574/150‑1 (MQ=21) gAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAgg > 1:1024574/1‑150 (MQ=21) gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGt > 2:628107/1‑150 (MQ=255) gcgcCGCGCGCGCGGCGTTCGATCCCCCAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACCCCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGc < 2:726560/150‑1 (MQ=255) gcgcCGCCCGCGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGc < 1:177373/150‑1 (MQ=255) cgcggcgCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCAt < 1:128375/149‑1 (MQ=255) gcTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACATCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAAtt > 2:317163/1‑150 (MQ=255) | TGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATT > NC_002947/1184464‑1184749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |