Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,207,681 T→C 6.9% Q442Q (CAA→CAG)  PP_1058 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,207,6810TC 6.9% 173.0 / 2.7 58Q442Q (CAA→CAG) PP_1058hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/29);  new base C (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGCGACCCA  >  NC_002947/1207538‑1207830
                                                                                                                                               |                                                                                                                                                      
ccATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAgg                                                                                                                                                  >  1:659540/1‑150 (MQ=255)
  aTCNGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTc                                                                                                                                                >  2:700258/1‑150 (MQ=255)
         gTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGc                                                                                                                                         >  2:127546/1‑150 (MQ=255)
           gCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCaa                                                                                                                                       <  1:297474/150‑1 (MQ=255)
            cGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGCTCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAg                                                                                                                                      <  2:934131/150‑1 (MQ=255)
              ttCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGt                                                                                                                                    >  1:722376/1‑150 (MQ=255)
                 gAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCCAGCAACTCCGGGGCGGCAAGGTTTTTCACGTCCTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACt                                                                                                                                 >  1:748525/1‑150 (MQ=255)
                    cAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACtg‑gt                                                                                                                             >  1:136764/1‑150 (MQ=255)
                            aGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACa                                                                                                                     <  1:1100841/150‑1 (MQ=255)
                            aGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACa                                                                                                                     <  2:136764/150‑1 (MQ=255)
                             gCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAg                                                                                                                    <  1:146405/150‑1 (MQ=255)
                                  aGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCCGTAAGGTACTG‑GTGGCTGACAGGTCGa                                                                                                               <  1:703661/150‑1 (MQ=255)
                                            gTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAg                                                                                                     <  1:127546/150‑1 (MQ=255)
                                               ggtggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTc                                                                                                  >  1:221701/1‑150 (MQ=255)
                                                gtggtCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCg                                                                                                 <  2:111093/150‑1 (MQ=255)
                                                    tCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACa                                                                                             >  1:682159/1‑150 (MQ=255)
                                                    tCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAACTGCAGGttagc                                                                                                                                             >  1:1127638/1‑99 (MQ=255)
                                                    tCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAACTGCAGGttagc                                                                                                                                             <  2:1127638/103‑5 (MQ=255)
                                                         gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCg                                                                                                           <  2:263586/131‑1 (MQ=255)
                                                         gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCg                                                                                                           >  1:263586/1‑131 (MQ=255)
                                                         gCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCggg                                                                                        >  1:81166/1‑150 (MQ=255)
                                                                gcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGa                                                                                 <  1:377660/150‑1 (MQ=255)
                                                                gcagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGa                                                                                 <  2:659540/150‑1 (MQ=255)
                                                                 cagcCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAg                                                                                <  2:748525/150‑1 (MQ=255)
                                                                      gCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATgg                                                                           <  2:221701/150‑1 (MQ=255)
                                                                      gCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATgg                                                                           >  1:95764/1‑150 (MQ=255)
                                                                               tCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGc                                                                  <  1:1104270/150‑1 (MQ=255)
                                                                                  ggCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAg                                                                                                     >  1:647860/1‑112 (MQ=255)
                                                                                  ggCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAg                                                                                                     <  2:647860/112‑1 (MQ=255)
                                                                                  ggCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGt                                                               >  1:764704/1‑150 (MQ=255)
                                                                                   gCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTCCCGGTCATCGCCGGGTTGCAGTTGCAGGTCGTTCGGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGAGTGAGCATGGCCGACAGGCGGtt                                                              >  1:748989/1‑150 (MQ=255)
                                                                                     cGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTgc                                                            >  2:642349/1‑150 (MQ=255)
                                                                                                 cAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGa                                                <  2:764704/150‑1 (MQ=255)
                                                                                                      tCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGccc                                           >  2:490710/1‑150 (MQ=255)
                                                                                                            tCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTAC‑ACATCCTATGTCGCCTTTAGTGCAATTCGGTCGAGTGCGTACAGCGGGCGATTGAGACTGGCCGATATGCTGTTGCGCGCCAGGCTGATGCCCttgttg                                     <  1:252619/150‑1 (MQ=255)
                                                                                                              ggCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGt                                   >  1:612264/1‑150 (MQ=255)
                                                                                                              ggCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGt                                   >  1:34980/1‑150 (MQ=255)
                                                                                                               gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgt                                       <  1:404433/145‑1 (MQ=255)
                                                                                                               gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCttgt                                       >  2:404433/1‑145 (MQ=255)
                                                                                                               gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTa                                  >  2:760517/1‑150 (MQ=255)
                                                                                                               gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTa                                  <  2:817968/150‑1 (MQ=255)
                                                                                                               gCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTa                                  >  1:378666/1‑150 (MQ=255)
                                                                                                                cAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAGGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGGCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTaa                                 >  1:799403/1‑150 (MQ=255)
                                                                                                                cAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTaa                                 <  1:700258/150‑1 (MQ=255)
                                                                                                                 aaGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAAt                                >  1:1002386/1‑150 (MQ=255)
                                                                                                                 aaGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAAt                                <  2:748989/150‑1 (MQ=255)
                                                                                                                      ttttCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCg                           <  1:642349/150‑1 (MQ=255)
                                                                                                                       tttCAGGTACTGTCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCgg                          <  1:725399/150‑1 (MQ=255)
                                                                                                                            ggTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGa                     <  2:81166/150‑1 (MQ=255)
                                                                                                                              tACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATg                   <  2:49168/150‑1 (MQ=255)
                                                                                                                              tACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATg                   <  2:682159/150‑1 (MQ=255)
                                                                                                                                 tGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTc                <  1:760517/150‑1 (MQ=255)
                                                                                                                                  ggCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCg               <  2:587231/150‑1 (MQ=255)
                                                                                                                                   gccgaCCTGCAACTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGc                                                                  <  2:391266/94‑1 (MQ=255)
                                                                                                                                   gccgaCCTGCAACTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGc                                                                  >  1:391266/5‑98 (MQ=255)
                                                                                                                                   gCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTc                                                                                                  >  1:937059/1‑66 (MQ=255)
                                                                                                                                   gCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTc                                                                                                  <  2:937059/66‑1 (MQ=255)
                                                                                                                                    cTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGc             >  2:923086/1‑150 (MQ=255)
                                                                                                                                               ttGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGCGACCCa  >  2:143395/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                      
CCATCGGCGGTGCGTTCGAACAAGGTGAAGCTGTAGCTCACCTGGTCGGTGGTCTTGGCAGTGAGCAGCCGCTCGCCGATCAGGCCGATCTGTGCGGCAAACTCCGGGTCGGCAAGGTTTTTCAGGTACTGGCTAACCTGCAGTTGCAGGTCGGCCTGCAAGGTACTG‑GTGGCTGACAGGTCGAGGCGGTCGAGGTCGTACAGCGGGCGATTGAGCATGGCCGACAGGCGGTTGCGCGCCAGGCTGATGCCCTTGTTGGTAATGATCGGTACGATGGTCGGCTGCGCGACCCA  >  NC_002947/1207538‑1207830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑