| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,281,200 | A→T | 20.5% | intergenic (+20/+23) | PP_5742 → / ← PP_1118 | membrane protein/recombinase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,281,200 | 0 | A | T | 20.5% | 82.7 / 18.0 | 39 | intergenic (+20/+23) | PP_5742/PP_1118 | membrane protein/recombinase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (16/15); new base T (5/3); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
TATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTGAA > NC_002947/1281061‑1281341 | tATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCCTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCta > 2:620284/1‑150 (MQ=255) gACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGACCTTGGAGAGCCCGCCATAAGGCGGGTCtatta < 1:1051102/150‑1 (MQ=255) aCTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCtattat < 1:957961/150‑1 (MQ=255) tgtgATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACg < 1:757708/150‑1 (MQ=255) aaTTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTAttt > 1:884194/1‑150 (MQ=255) gaagaCTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCCTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGc < 1:620284/150‑1 (MQ=255) aagaCTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCt < 2:1034829/150‑1 (MQ=255) gaCTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCttt > 1:70441/1‑150 (MQ=255) tGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGc < 2:70441/150‑1 (MQ=255) tGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTccc > 1:359795/1‑150 (MQ=255) ttGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGACGGGTGTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATTTGAGGCGGTAGACTGTCGCAAgg > 2:150852/1‑149 (MQ=255) aTCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCa < 2:574829/150‑1 (MQ=255) aTCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCa > 2:321543/1‑150 (MQ=255) aTCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTCTCCACATGGCTAGCATGTGAGGCGGTCGAATGTCCCCAGAGTCa < 2:884194/150‑1 (MQ=255) aGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTAtt > 1:1105485/1‑82 (MQ=255) aGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTAtt < 2:1105485/82‑1 (MQ=255) ccGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCt > 2:60087/1‑90 (MQ=255) ccGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCt < 1:60087/90‑1 (MQ=255) cGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAg < 1:321543/150‑1 (MQ=255) gggAAGCTAGACCAAAGTGAATTAAGAGAGCCCGCCATAAGGCGGGTGTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGTGGCTTcc > 2:571608/1‑150 (MQ=255) ggAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCg < 1:1036178/108‑1 (MQ=255) ggAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCg > 2:1036178/1‑108 (MQ=255) gAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCAc > 2:110072/1‑150 (MQ=255) gCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTc > 1:493055/1‑150 (MQ=255) cAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAgg > 2:319640/1‑150 (MQ=255) cTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGc > 1:745086/1‑150 (MQ=255) aaTTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGa > 1:373838/1‑51 (MQ=255) aaTTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGa < 2:373838/51‑1 (MQ=255) ttAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGTTAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTgg > 1:1049698/1‑150 (MQ=255) ttAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTgg < 2:745086/150‑1 (MQ=255) taatagacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGATTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGt > 1:341218/8‑150 (MQ=255) tagacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAgg > 2:241692/5‑136 (MQ=255) tagacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAgg > 2:241853/5‑136 (MQ=255) tagacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAgg < 1:241692/132‑1 (MQ=255) tagacccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAgg < 1:241853/132‑1 (MQ=255) acccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGt > 2:10320/2‑82 (MQ=255) acccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGt < 1:10320/81‑1 (MQ=255) acccGCCTTATGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGAGTCAACTg > 2:611367/2‑150 (MQ=255) ccGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTAATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTNTTGGNAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTGaa < 1:150852/150‑1 (MQ=255) | TATGACTTGATCTGTGATAATTCTGTTGTTGCAGAAGACTATGTGTTTTTGACAGAGATGGGGCGGGAGTATGTCTTGCATCCACAGAAGCCGCTTCTATGGGAAGCTAGACCAAACTGAATTAAGAGAGCCCGCCATAAGGCGGGTCTATTATGAGTCACGTTATTTGATAATATGTCTGGCTTTCCACATGGCTAGCATGTGAGGCGGTAGACTGTCCCAAGATTCAGTTTTGGCAAAGGCGGCTTCCACCTCGTTCAGGTCGCCTTGGGTCAACTGAA > NC_002947/1281061‑1281341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |