Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,417,615 G→A 8.5% R23H (CGC→CAC)  purC → phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,417,6150GA 8.5% 128.5 / 3.2 47R23H (CGC→CAC) purCphosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base G (25/18);  new base A (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

GCTCCAGGTGGCGAATCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCGGCGTGCCGACCCAGTTC  >  NC_002947/1417466‑1417757
                                                                                                                                                     |                                                                                                                                              
gCTCCAGGTGGCGAATCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCg                                                                                                                                                >  1:199866/1‑150 (MQ=255)
  tCCAGGTGGCGAATCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGCCGACGCCGACCGCt                                                                                                                                              >  2:473075/1‑150 (MQ=255)
     atgTGGCGACTCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACGCCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTGTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGa                                                                                                                                           <  2:131849/148‑1 (MQ=255)
       gTGGCGAATCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATc                                                                                                                                         >  2:930662/1‑150 (MQ=255)
             aaTCAGGAACACGGGCGCGACTGGCGCGAAATCGCCTAGCCCAACACGTCAAGCAAGCGGAGCCCAATCCTGGAAAAACGCGAAGAACTTTACCGCGGCACGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGACCctgctg                                                                                                                                   >  2:571200/1‑150 (MQ=255)
              aTCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGt                                                                                                                                  <  1:1106567/150‑1 (MQ=255)
               tCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAGGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGtt                                                                                                                                 >  1:67032/1‑150 (MQ=255)
                 aGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAGGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTcc                                                                                                                               <  2:67032/150‑1 (MQ=255)
                 aGGAACACGGGCTCGACTGGCGCGAAATCGCCTACCACATCCCCTCGTGCAAGCGGAGCCCAATCATGGAAAAACGCGATGAACTTTACCGCGGCCAGGCCAAATCGGCTTACAAGACCTCCGATGCCGACTGCTTGCTCCTGCTGTTcc                                                                                                                               <  2:514116/150‑1 (MQ=255)
                  ggAACACGGGCTCGACTGGCGCGGAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCtt                                                                                                                                             <  1:899946/135‑1 (MQ=255)
                  ggAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCtt                                                                                                                                             >  2:899946/1‑135 (MQ=255)
                   gAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGt                                                                                                                             <  2:105722/150‑1 (MQ=255)
                   gAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGt                                                                                                                             >  2:772307/1‑150 (MQ=255)
                               gACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCg                                                                                                                 <  1:45867/150‑1 (MQ=255)
                                  tGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGCAGCGGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCg                                                                                                              >  1:11100/1‑150 (MQ=255)
                                      gcgAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGCCGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCg                                                                                                          >  2:675789/1‑150 (MQ=255)
                                       cgAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGa                                                                                                         <  1:706420/150‑1 (MQ=255)
                                         aaaTCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACg                                                                                                       <  2:485043/150‑1 (MQ=255)
                                             cGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCaa                                                                                                   <  1:772307/150‑1 (MQ=255)
                                               ccTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGc                                                                                                 >  2:1045527/1‑150 (MQ=255)
                                                cTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCg                                                                                                >  2:334539/1‑150 (MQ=255)
                                                    cccAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATc                                                                                            >  1:428972/1‑150 (MQ=255)
                                                     ccAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCg                                                                                           <  1:1045527/150‑1 (MQ=255)
                                                      cAACACCTCAAGCAAGCGGAGCCCAATCAGGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGa                                                                                          >  1:753574/1‑150 (MQ=255)
                                                       aaCACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGaa                                                                                         >  2:153254/1‑150 (MQ=255)
                                                            cTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACt                                                                                    >  1:29083/1‑150 (MQ=255)
                                                             tCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTc                                                                                   >  2:1078820/1‑150 (MQ=255)
                                                             tCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTc                                                                                   >  1:510435/1‑150 (MQ=255)
                                                             tCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTc                                                                                   >  2:1117139/1‑150 (MQ=255)
                                                               aagcaagcGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGCCGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGa                                                                                 <  1:571200/150‑1 (MQ=255)
                                                                  caagcGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCg                                                                              <  2:199866/150‑1 (MQ=255)
                                                                                  acggAAAGACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGaac                                                              >  2:227417/3‑150 (MQ=255)
                                                                                   tGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGaaca                                                             >  1:615445/1‑150 (MQ=255)
                                                                                        aaaCGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAg                                                        <  1:83899/150‑1 (MQ=255)
                                                                                                     tttACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTtca                                           >  2:330279/1‑150 (MQ=255)
                                                                                                                     cAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGga                           <  2:153082/150‑1 (MQ=255)
                                                                                                                      aaaTCGGTTTACAAGACCGACGACGCCGACCACTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGaacaa                                                            <  2:453689/116‑1 (MQ=255)
                                                                                                                      aaaTCGGTTTACAAGACCGACGACGCCGACCACTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGaacaa                                                            >  1:452533/1‑116 (MQ=255)
                                                                                                                      aaaTCGGTTTACAAGACCGACGACGCCGACCACTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGaacaa                                                            >  1:453689/1‑116 (MQ=255)
                                                                                                                      aaaTCGGTTTACAAGACCGACGACGCCGACCACTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGaacaa                                                            <  2:452533/116‑1 (MQ=255)
                                                                                                                        aTCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGgaaga                        >  1:791958/1‑150 (MQ=255)
                                                                                                                             tttACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCg                   <  1:153254/150‑1 (MQ=255)
                                                                                                                                 cAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGacac                                                                                                                     <  2:442182/48‑1 (MQ=255)
                                                                                                                                 cAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGacac                                                                                                                     >  1:442182/1‑48 (MQ=255)
                                                                                                                                  aaGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCGGCGTg              >  2:497673/1‑150 (MQ=255)
                                                                                                                                   aGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCGGCGTGc             <  1:633323/150‑1 (MQ=255)
                                                                                                                                    gACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCGGCGTGcc            >  1:800378/1‑150 (MQ=255)
                                                                                                                                              gCCGACCGCTTGATCATGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCGGCGTGCCGACCCAGTTc  >  2:376093/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GCTCCAGGTGGCGAATCAGGAACACGGGCTCGACTGGCGCGAAATCGCCTAGCCCAACACCTCAAGCAAGCGGAGCCCAATCATGGAAAAACGCGAAGAACTTTACCGCGGCAAGGCCAAATCGGTTTACAAGACCGACGACGCCGACCGCTTGATCCTGCTGTTCCGTAACGACACGTCGGCGTTCGACGGCAAGCGTATCGAACAACTCGACCGCAAAGGCATGGTGAACAACAAGTTCAACGCCTTCATCATGCAGAAACTGGAAGAAGCCGGCGTGCCGACCCAGTTC  >  NC_002947/1417466‑1417757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑