Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,551,327 A→C 7.9% intergenic (+44/+82) groL → / ← pykA chaperonin GroEL/pyruvate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,551,3270AC 7.9% 176.3 / 7.2 63intergenic (+44/+82)groL/pykAchaperonin GroEL/pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base A (28/30);  new base C (3/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

CACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGT  >  NC_002947/1551187‑1551468
                                                                                                                                            |                                                                                                                                             
caccacCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgggg                                                                                                                                      <  2:664507/150‑1 (MQ=255)
 accaccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGc                                                                                                                                     <  1:568698/150‑1 (MQ=255)
  ccaccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgg                                                                                                                                        <  2:896816/146‑1 (MQ=255)
  ccaccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgg                                                                                                                                        >  1:896816/1‑146 (MQ=255)
  ccaccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCt                                                                                                                                    >  1:851385/1‑150 (MQ=255)
     ccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttt                                                                                                                                 <  1:906454/150‑1 (MQ=255)
       gAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttttt                                                                                                                               <  2:899286/150‑1 (MQ=255)
             aTGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATCATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCt                                                                                                                         >  2:371345/1‑150 (MQ=255)
                  tGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTCAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCgg                                                                                                                    <  1:136534/150‑1 (MQ=255)
                     cGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc                                                                                                                 >  1:1005008/1‑150 (MQ=255)
                                   aCAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGcaca                                                                                                   >  1:843064/1‑150 (MQ=255)
                                        ccAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCt                                                                                              >  1:729598/1‑150 (MQ=255)
                                        ccAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCt                                                                                              >  2:1071225/1‑150 (MQ=255)
                                             tgctgGCATTCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCt                                                                                         >  2:885054/1‑150 (MQ=255)
                                             tgctgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCt                                                                                         >  2:609812/1‑150 (MQ=255)
                                                       ccTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttt                                                                                                                                 <  1:32776/100‑1 (MQ=255)
                                                       ccTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttt                                                                                                                                 >  2:32776/1‑100 (MQ=255)
                                                          gATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTa                                                                                                                              <  2:614255/100‑1 (MQ=255)
                                                          gATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTa                                                                                                                              >  1:614255/1‑100 (MQ=255)
                                                           atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttt                                                                                                                                 <  2:191441/96‑1 (MQ=255)
                                                           atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtttt                                                                                                                                 >  1:191441/1‑96 (MQ=255)
                                                           atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtg                                                                           <  1:1071225/150‑1 (MQ=255)
                                                           atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCCTGAGCCTTGTGCtg                                                                           >  2:1070985/1‑150 (MQ=255)
                                                                ggtggcatgggtggcatgGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtg                                                                           <  1:940604/145‑1 (MQ=255)
                                                                ggtggcatgggtggcatgGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtg                                                                           >  2:940604/1‑145 (MQ=255)
                                                                 gtggcatgggtggcatggGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAAc                                                                     <  2:1005008/150‑1 (MQ=255)
                                                                    gcatgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAACAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg                                                                  <  1:491407/150‑1 (MQ=255)
                                                                    gcatgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg                                                                  >  2:804835/1‑150 (MQ=255)
                                                                     catgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGt                                                                 >  1:518069/1‑150 (MQ=255)
                                                                      atgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTg                                                                >  2:834810/1‑150 (MQ=255)
                                                                      atgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTg                                                                <  1:434986/150‑1 (MQ=255)
                                                                                       cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCt                                                                                              <  2:260949/103‑1 (MQ=255)
                                                                                       cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCt                                                                                              >  1:260949/1‑103 (MQ=255)
                                                                                       cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGt                                                                 <  2:45939/132‑1 (MQ=255)
                                                                                       cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGt                                                                 >  1:45939/1‑132 (MQ=255)
                                                                                             gTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCAcc                                         <  1:371345/150‑1 (MQ=255)
                                                                                                agccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGAc                                      <  2:729598/150‑1 (MQ=255)
                                                                                                 gccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGa                                                              >  2:318727/1‑125 (MQ=255)
                                                                                                 gccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGa                                                              <  1:318727/125‑1 (MQ=255)
                                                                                                 gccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACg                                     >  1:978185/1‑150 (MQ=255)
                                                                                                  ccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGt                                    <  1:538464/150‑1 (MQ=255)
                                                                                                  ccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGt                                    <  2:361286/150‑1 (MQ=255)
                                                                                                     gccTTACCCCCCTGCACCATCAAACAGCCCCGGTTCGCGAGAACCGGGACTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGGGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCa                                 <  1:268975/150‑1 (MQ=255)
                                                                                                     gccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGa                                       <  2:1049214/144‑1 (MQ=255)
                                                                                                     gccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGa                                       >  1:1049214/1‑144 (MQ=255)
                                                                                                      ccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAg                                <  1:326853/150‑1 (MQ=255)
                                                                                                          aCCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTc                                                                                                                      <  2:1090742/60‑1 (MQ=255)
                                                                                                          aCCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTc                                                                                                                      >  1:1090742/1‑60 (MQ=255)
                                                                                                             ccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTc                         <  1:834810/150‑1 (MQ=255)
                                                                                                               ccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCAt                       <  2:107994/150‑1 (MQ=255)
                                                                                                                      cATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCCTTg                >  2:1084639/1‑150 (MQ=255)
                                                                                                                          aaaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGc            >  2:819194/1‑150 (MQ=255)
                                                                                                                             aaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCAc         <  1:819194/150‑1 (MQ=255)
                                                                                                                                ccccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGAt                                                             <  1:403452/95‑1 (MQ=255)
                                                                                                                                ccccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGAt                                                             >  2:403452/1‑95 (MQ=255)
                                                                                                                                ccccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGa      <  1:885054/150‑1 (MQ=255)
                                                                                                                                 cccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGa                                                      >  2:101082/1‑101 (MQ=255)
                                                                                                                                 cccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGa                                                      <  1:101082/101‑1 (MQ=255)
                                                                                                                                 cccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt     >  1:744460/1‑150 (MQ=255)
                                                                                                                                 cccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt     >  1:679449/1‑150 (MQ=255)
                                                                                                                                 cccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt     >  1:582667/1‑150 (MQ=255)
                                                                                                                                 cccGGATCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGTTGCCACCGAt     >  1:1016989/1‑150 (MQ=255)
                                                                                                                                    ggTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGTGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGgt  <  1:853280/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
CACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGT  >  NC_002947/1551187‑1551468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑