Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,625,528	G→T	7.5%	V451V (GTC→GTA)	nadB ←	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,625,528	0	G	T	7.5%	190.2 / 2.1	62	V451V (GTC→GTA)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base G (31/25);  new base T (3/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTGCGCCTGGATGTCGGCAGCGGC  >  NC_002947/1625380‑1625677                                                                                                                                                     |                                                                                                                                                      tcatcaGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGa                                                                                                                                                      >  2:141740/1‑150 (MQ=255) tcatcaGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGa                                                                                                                                                      <  2:56707/150‑1 (MQ=255)    tcaGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGt                                                                                                                                                   >  1:1106730/1‑150 (MQ=255)    tcaGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGt                                                                                                                                                   >  1:1115715/1‑150 (MQ=255)       gcccGGCAACTTGCGCAAGTTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGt                                                                                                                                                >  2:800927/4‑150 (MQ=255)       gCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGt                                                                                                                                                >  2:115364/1‑150 (MQ=255)       gCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCAACGTAGt                                                                                                                                                >  1:741576/1‑150 (MQ=255)       gCTCGGCCAATTCGGACAGCGTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGt                                                                                                                                                >  2:800859/1‑150 (MQ=255)             ccACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCcaca                                                                                                                                          <  1:351860/150‑1 (MQ=255)             ccACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCACCGAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCcaca                                                                                                                                          >  2:317412/1‑150 (MQ=255)                cTTGCGCCGGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGACGACGTAGTCCCACATGa                                                                                                                                       <  1:37986/150‑1 (MQ=255)                     gcCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAgcgc                                                                                                                                  >  2:282382/1‑150 (MQ=255)                         ggTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCa                                                                                                                              <  1:796911/150‑1 (MQ=255)                               gcAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGt                                                                                                                        <  2:261563/150‑1 (MQ=255)                                  gCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTccc                                                                                                                     >  1:511552/1‑150 (MQ=255)                                  gCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTccc                                                                                                                     >  1:625431/1‑150 (MQ=255)                                      gATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGtt                                                                                                                 >  1:892332/1‑150 (MQ=255)                                         cAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTtgtg                                                                                                              <  2:1106730/150‑1 (MQ=255)                                                  gCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACCTGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGAt                                                                                                     >  2:88800/1‑150 (MQ=255)                                                      aCCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACg                                                                                                 <  2:833845/150‑1 (MQ=255)                                                        cTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTc                                                                                               <  2:625431/150‑1 (MQ=255)                                                                    gTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCTACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTc                                                                                   >  1:218458/1‑150 (MQ=255)                                                                    gTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCTACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTc                                                                                   >  1:218450/1‑150 (MQ=255)                                                                     tAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCg                                                                                  <  2:1115715/150‑1 (MQ=255)                                                                           tcgtcgATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCAcc                                                                            >  1:220179/1‑150 (MQ=255)                                                                           tcgtcgATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTGGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCAcc                                                                            >  2:107576/1‑150 (MQ=255)                                                                                gATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACctggct                                                                       >  2:806113/1‑150 (MQ=255)                                                                                  tttCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATc                                                                                                    <  2:1017126/119‑1 (MQ=255)                                                                                  tttCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATc                                                                                                    >  1:1017126/1‑119 (MQ=255)                                                                                   ttCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCtggctggc                                                                    <  1:141740/150‑1 (MQ=255)                                                                                   gttctacagcagcagGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCtggctggc                                                                    <  1:795240/144‑1 (MQ=255)                                                                                      aTCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTc                                                                 >  1:260982/1‑150 (MQ=255)                                                                                       tCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACg                                                                                                                                                    >  2:1114373/1‑66 (MQ=255)                                                                                       tCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACg                                                                                                                                                    <  1:1114373/66‑1 (MQ=255)                                                                                         cagcagcagGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCa                                                              >  1:421207/1‑150 (MQ=255)                                                                                         cagcagcagGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCa                                                              <  1:115364/150‑1 (MQ=255)                                                                                          agcagcagGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAg                                                             >  1:1038833/1‑150 (MQ=255)                                                                                              gcagGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCTACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCgg                                                         <  2:218450/150‑1 (MQ=255)                                                                                              gcagGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCTACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCgg                                                         <  2:218458/150‑1 (MQ=255)                                                                                                  gCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCa                                                                                                   >  1:783467/1‑104 (MQ=255)                                                                                                  gCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCa                                                                                                   <  2:783467/104‑1 (MQ=255)                                                                                                  gCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTc                                                                                         >  1:839566/1‑114 (MQ=255)                                                                                                  gCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTc                                                                                         <  2:839566/114‑1 (MQ=255)                                                                                                  gCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCaa                                                     >  2:918028/1‑150 (MQ=255)                                                                                                  gCGAATGCGGTGCTGGGCCCGCTACAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCaa                                                     >  1:225379/1‑150 (MQ=255)                                                                                                   cGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAg                                                    <  1:918028/150‑1 (MQ=255)                                                                                                   cGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAg                                                    <  2:220179/150‑1 (MQ=255)                                                                                                          ggTGCTGGGCCCGCTACAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTgg                                             <  2:225379/150‑1 (MQ=255)                                                                                                                gggCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATgg                                       >  1:1054260/1‑150 (MQ=255)                                                                                                                        tGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGc                               <  2:347419/150‑1 (MQ=255)                                                                                                                          cAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACt                                  <  2:282241/145‑1 (MQ=255)                                                                                                                          cAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACt                                  >  1:282241/1‑145 (MQ=255)                                                                                                                          cAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTc                             <  2:741576/150‑1 (MQ=255)                                                                                                                            ggCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCa                           <  1:614618/150‑1 (MQ=255)                                                                                                                             gcgcTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGAGCAGCAGATCGTCCGAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAg                          >  1:412689/1‑150 (MQ=255)                                                                                                                               gcTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGt                        >  2:1128409/1‑150 (MQ=255)                                                                                                                                cTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTg                       >  1:269131/1‑150 (MQ=255)                                                                                                                                 ttGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTgc                      >  2:246299/1‑150 (MQ=255)                                                                                                                                     tGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTGCGCCt                  <  1:690432/150‑1 (MQ=255)                                                                                                                                        tGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTGCGCCTGGa               <  1:88800/150‑1 (MQ=255)                                                                                                                                           gaatgATGCCTACGTAGTCCCACATGAAGCGCCACAGTTCGACCCAGGTGTGCGCAATGAACACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCCGCCGGGCAGGGCGTTGGGCATGGCCACTTTCTCCAGGTGCGCCTGGAGGt            >  2:219111/5‑150 (MQ=255)                                                                                                                                            cACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTGCGCCTGGATGTc           >  1:1035797/1‑150 (MQ=255)                                                                                                                                                     gACGTAGTCCCACATGAAGCTCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTGCGCCTGGATGTCGGCAgcggc  >  1:456634/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      TCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCGTCGATTTCATCCAGCAGCAGGCGAATGCGGTGCTGGGCCCGCTGCAGGCGCTTGCTGGTGCGCACGATGCCGACGTAGTCCCACATGAAGCGCCGCAGTTCGTCCCAGTTGTGCGCAATGATCACGTCCTCGTCCGAGTCGGTCACCTGGCTGGCGTCCCAGCCGGGCAAGGCCTTGGGCATGGCCACTTGCTCCAGGTGCGCCTGGATGTCGGCAGCGGC  >  NC_002947/1625380‑1625677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑