Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,632,139	A→G	12.9%	E284G (GAG→GGG)	lepA →	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,632,139	0	A	G	12.9%	126.7 / 9.5	55	E284G (GAG→GGG)	lepA	elongation factor 4
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base G (2/5);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

GCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGA  >  NC_002947/1631990‑1632283                                                                                                                                                      |                                                                                                                                                  gCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGa                                                                                                                                                   >  1:615288/1‑150 (MQ=255)   aCTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAgg                                                                                                                                                 >  2:119724/1‑150 (MQ=255)       ggTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGTCATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGa                                                                                                                                             <  2:1034158/150‑1 (MQ=255)        gTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGAg                                                                                                                                            <  1:312212/150‑1 (MQ=255)        gTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCCAg                                                                                                                                            <  2:885261/150‑1 (MQ=255)         tCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAgg                                                                                                                                           >  1:1078394/1‑150 (MQ=255)         tCGACAGCGGCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCGGCATCAAGGACCTCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGCgg                                                                                                                                           >  2:503980/1‑150 (MQ=255)         tCGACAGCATCGGTGTCTTCACCCCGAAGCACACCTAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCTCAGGGGTCGAgg                                                                                                                                           <  1:190540/150‑1 (MQ=255)                 gTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCa                                                                                                                                   <  2:1078394/150‑1 (MQ=255)                     gtgtCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCt                                                                                                                               >  2:312090/1‑150 (MQ=255)                          ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaag                                                                                                                          >  2:52179/1‑150 (MQ=255)                          ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaag                                                                                                                          >  2:530949/1‑150 (MQ=255)                          ttCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCaag                                                                                                                          >  1:791053/1‑150 (MQ=255)                           tCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGgt                                                                                                                                          >  2:554230/1‑133 (MQ=255)                           tCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGgt                                                                                                                                          <  1:554230/133‑1 (MQ=255)                              ccccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAag‑aaga                                                                                                                     <  1:531652/150‑1 (MQ=255)                               cccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGAt                                                                                                                    >  2:650634/1‑150 (MQ=255)                                ccGAAGCACACCCAGCCCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGCCCCCTGAGGTCGAGgtgt                                                                                                                                        <  1:43349/130‑1 (MQ=255)                                ccGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGgtgt                                                                                                                                        >  2:43349/1‑130 (MQ=255)                                     gCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGcc                                                                                                              <  1:312090/150‑1 (MQ=255)                                          cccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAgg                                                                                                         >  2:80337/1‑150 (MQ=255)                                          cccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAgg                                                                                                         <  1:116869/150‑1 (MQ=255)                                          cccAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAgg                                                                                                         >  2:391182/1‑150 (MQ=255)                                              gACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACCTCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTa                                                                                                     <  2:979868/150‑1 (MQ=255)                                                     gATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGc                                                                                              <  2:880546/150‑1 (MQ=255)                                                       tCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGAGACCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCt                                                                                            >  1:361068/1‑150 (MQ=255)                                                            aaGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGAAATACCCAGTCCCCCGAGGTTTGACACCCTGACGCGGGCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTcc                                                                                       >  1:982130/1‑150 (MQ=255)                                                                  gCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCa                                                                                 <  1:52179/150‑1 (MQ=255)                                                                   cGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGTTCAg                                                                                <  2:1093170/150‑1 (MQ=255)                                                                          ggCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCcgac                                                                         <  1:650634/150‑1 (MQ=255)                                                                                atcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTc                                                                   <  1:119724/150‑1 (MQ=255)                                                                                atcatcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCG‑GGAAAAAGTGGGATTGATGCATTAGCGAACGGCTTGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTc                                                                   >  1:406921/1‑150 (MQ=255)                                                                                   atcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                >  1:1064781/1‑150 (MQ=255)                                                                                   atcGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGACTACGCCGGCCGGTTCCCGGTCAGCTCCGACGACTTCGAg                                                                >  2:932178/1‑150 (MQ=255)                                                                                       ccAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAag‑aaga                                                                                                                     >  1:26424/1‑93 (MQ=255)                                                                                       ccAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAag‑aaga                                                                                                                     <  2:26424/93‑1 (MQ=255)                                                                                          gCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTcc                                                         >  2:100293/1‑150 (MQ=255)                                                                                               aaGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGAc                                                    <  2:1064781/150‑1 (MQ=255)                                                                                                 ggACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGc                                                  >  1:778410/1‑150 (MQ=255)                                                                                                    cATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGctct                                               <  1:768012/150‑1 (MQ=255)                                                                                                            gTGCCCCGGTTGGTGACACCCTGACCCTTGCGTTGACCCCTGAGGTCGAGGTGTTGCCATGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCTGCCTGTTCCCGGTCAGCTCCGACGACGTCGAGGATTTCCGCGACGCTCTGCAGAAGc                                       <  1:503980/150‑1 (MQ=255)                                                                                                                      tGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGaa                             <  2:982130/150‑1 (MQ=255)                                                                                                                          gACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGAc                         <  2:536035/150‑1 (MQ=255)                                                                                                                             accctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTg                                              <  1:838627/126‑1 (MQ=255)                                                                                                                             accctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTg                                              >  2:838627/1‑126 (MQ=255)                                                                                                                             accctgaccctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGActcc                      >  2:973222/1‑150 (MQ=255)                                                                                                                                     cctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCg                   >  2:590759/1‑145 (MQ=255)                                                                                                                                     cctgTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCg                   <  1:590759/145‑1 (MQ=255)                                                                                                                                      aacaccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAg             <  1:804224/146‑1 (MQ=255)                                                                                                                                       acaccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCg                                                                                     <  2:376345/74‑1 (MQ=37)                                                                                                                                       acaccTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCg                                                                                     >  1:376345/4‑77 (MQ=37)                                                                                                                                         accTCGACCTCAGGGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTAc          >  1:357288/2‑150 (MQ=255)                                                                                                                                            tCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATg       >  2:940636/1‑150 (MQ=255)                                                                                                                                                 cccTGCGGACGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGa  >  2:347220/1‑150 (MQ=255)                                                                                                                                                  ccTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGAc                         >  1:815679/1‑126 (MQ=255)                                                                                                                                                  ccTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGAc                         <  2:815679/126‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GCACTTGGTCGACAGCGTCGGTGTCTTCACCCCGAAGCACACCCAGACCGCTGATCTGAAAGCCGGCGAAGTAGGCTTCATCATCGCCAGCATCAAGGACATCCACGGTGCCCCGGTTGGTGACACCCTGACCCTGTCCTCGACCCCTGAGGTCGAGGTGTTGCCAGGCTTCAAG‑AAGATTCAGCCGCAGGTCTACGCCGGCCTGTTCCCGGTCAGCTCCGACGACTTCGAGGATTTCCGCGACGCTCTGCAGAAGCTGACCCTGAACGACTCCTCGCTGCAGTACATGCCGGA  >  NC_002947/1631990‑1632283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑