Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,637,386 C→T 9.8% intergenic (+53/+13) pdxJ → / ← PP_1437 pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,637,3860CT 9.8% 104.9 / 3.1 41intergenic (+53/+13)pdxJ/PP_1437pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (19/18);  new base T (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCG  >  NC_002947/1637250‑1637530
                                                                                                                                        |                                                                                                                                                
gcgcTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGAt                                                                                                                                     <  1:984606/150‑1 (MQ=255)
   cTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCAc                                                                                                                                  >  2:747012/1‑150 (MQ=255)
         gcgcATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCa                                                                                                                            <  2:327611/150‑1 (MQ=255)
         gcgcATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATAGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGGGAAGAGGGCAGCACAGGCGGCCGATCACCCGGCa                                                                                                                            >  2:364528/1‑150 (MQ=255)
           gcATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGTCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAgg                                                                                                                          >  2:688338/1‑150 (MQ=255)
                  ttgtttgtCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACAcc                                                                                                                   >  1:733291/1‑150 (MQ=255)
                   tgtttgtCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCa                                                                                                                  <  2:733291/150‑1 (MQ=255)
                            gCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGATGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCCATACCGGCCTTTTCGCAGGCAAGCCCTCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGACACAACAGGGTAAAcc                                                                                                         >  1:421928/1‑150 (MQ=255)
                              ttCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCgg                                                                                                       <  2:841740/150‑1 (MQ=255)
                                cAAGGCTGCTGTGGCCGACGAGAATCCTCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGCGTTTGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGt                                                                                                     <  1:1093102/150‑1 (MQ=255)
                                       gctgTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGcccc                                                                                              >  1:113535/1‑150 (MQ=255)
                                             gCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg                                                                                        <  1:747012/150‑1 (MQ=255)
                                                     gAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgct                                                                                <  2:113535/150‑1 (MQ=255)
                                                     gAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgct                                                                                <  2:490300/150‑1 (MQ=255)
                                                       aGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGACCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGa                                                                              <  1:688338/150‑1 (MQ=255)
                                                       aGGCGCTGATCGTGGCGGCTTCGCGCTGATTGGTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGa                                                                              <  1:1129756/150‑1 (MQ=255)
                                                        ttctcgtaTCGTGGCGGCTCCGCGCCGATTGTTCGTCGCATGTCCCGGCCGGTACGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGAc                                                                             <  2:1030598/143‑1 (MQ=255)
                                                           gcTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGa                                                                                                                                      >  1:590771/1‑90 (MQ=255)
                                                           gcTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGa                                                                                                                                      <  2:590771/90‑1 (MQ=255)
                                                             tGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGa                                                                        >  2:356689/1‑150 (MQ=255)
                                                                         cTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCAt                                                            <  1:616161/150‑1 (MQ=255)
                                                                               gcTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACt                                                      >  2:405538/1‑150 (MQ=255)
                                                                               gcTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACt                                                      >  2:219429/1‑150 (MQ=255)
                                                                                          gTCGCCTGTACCGGCCTTGTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGAc                                           <  2:356808/150‑1 (MQ=255)
                                                                                           tCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACt                                          >  1:379296/1‑150 (MQ=255)
                                                                                                 gtgctggCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGACCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgct                                                                                <  1:223541/101‑1 (MQ=17)
                                                                                                 gtgctggCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGACCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAgctgct                                                                                >  2:223541/6‑106 (MQ=17)
                                                                                                  tgctggCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGAt                                   >  1:1000465/5‑150 (MQ=38)
                                                                                                    ccGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCg                                 >  1:873134/1‑150 (MQ=255)
                                                                                                     cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGc                                >  1:1061542/1‑150 (MQ=255)
                                                                                                     cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTGCACGATCGc                                >  1:857341/1‑150 (MQ=255)
                                                                                                       gCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGcca                              >  2:903726/1‑150 (MQ=255)
                                                                                                          ttttCGCGGGCAAGCCCGCGAAGAGGCCAGTACAGGCGACCAATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAg                                                                                                   <  1:718876/78‑1 (MQ=37)
                                                                                                          ttttCGCGGGCAAGCCCGCGAAGAGGCCAGTACAGGCGACCAATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAg                                                                                                   >  2:718876/1‑78 (MQ=37)
                                                                                                          ttttCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaacc                           <  1:405538/150‑1 (MQ=255)
                                                                                                                      aGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGAcccgcc                                                                  <  1:970929/99‑1 (MQ=255)
                                                                                                                      aGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGAcccgcc                                                                  >  2:970929/1‑99 (MQ=255)
                                                                                                                               agagGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCCGCGATCATCTGCTGACCTCAACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCa      <  1:356689/150‑1 (MQ=255)
                                                                                                                                   gCCAGCACAGGCGGCCGAGCACTCGGCAGGAAAGACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCcg  >  2:206954/1‑150 (MQ=255)
                                                                                                                                     cAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTGAAGCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaacc                           <  1:69046/123‑1 (MQ=255)
                                                                                                                                     cAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaacc                           >  2:69046/1‑123 (MQ=255)
                                                                                                                                        |                                                                                                                                                
GCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAACCCGGTCCCGCCCTCCAGCCG  >  NC_002947/1637250‑1637530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑