Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,732,498 C→A 8.8% A472S (GCC→TCC)  PP_1526 ← beta‑(1‑3)‑glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,732,4980CA 8.8% 135.4 / 2.1 45A472S (GCC→TCC) PP_1526beta‑(1‑3)‑glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base A (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GCAGTAGTCCGAGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCT  >  NC_002947/1732355‑1732647
                                                                                                                                               |                                                                                                                                                     
gCAGTAGTCCGAGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAg                                                                                                                                                 >  2:304789/1‑150 (MQ=255)
         cGAGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGGTCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCca                                                                                                                                        >  1:793516/1‑150 (MQ=255)
          gAGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCcac                                                                                                                                       >  2:1126629/1‑150 (MQ=255)
           aGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGACTTGAGCGGCTCCcaca                                                                                                                                      >  2:928195/1‑150 (MQ=255)
           aGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGACTTGAGCGGCTCCcaca                                                                                                                                      >  2:361827/1‑150 (MQ=255)
               gATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGc                                                                                                                                  >  1:913168/1‑150 (MQ=255)
                aTTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGcc                                                                                                                                 >  1:781499/1‑150 (MQ=255)
                    ccGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGt                                                                                                                             >  2:897186/1‑150 (MQ=255)
                    ccGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGt                                                                                                                             >  2:108085/1‑150 (MQ=255)
                         aTCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAGTTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTg                                                                                                                        >  1:365095/1‑150 (MQ=255)
                         aTCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTg                                                                                                                        <  2:468369/150‑1 (MQ=255)
                         aTCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTg                                                                                                                        <  2:913168/150‑1 (MQ=255)
                         aTCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGACCGGGTCCTTg                                                                                                                        <  2:106443/150‑1 (MQ=255)
                                   cGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGtgttgttgt                                                                                                              <  1:1126629/150‑1 (MQ=255)
                                        tttGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTg                                                                                                                        <  1:975266/135‑1 (MQ=255)
                                        tttGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTg                                                                                                                        >  2:975266/1‑135 (MQ=255)
                                         ttGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCa                                                                                                        <  1:108085/150‑1 (MQ=255)
                                                   cGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTc                                                                                              <  1:1008533/150‑1 (MQ=255)
                                                         cAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGTACGCAGACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTc                                                                                        <  1:577069/150‑1 (MQ=255)
                                                             tAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGACTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCgggg                                                                                    <  1:361827/150‑1 (MQ=255)
                                                             tAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGACTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCgggg                                                                                    <  1:928195/150‑1 (MQ=255)
                                                               aTTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTa                                                                                  <  1:973605/150‑1 (MQ=255)
                                                                   aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCgggg                                                                                    >  2:51805/1‑144 (MQ=255)
                                                                   aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCgggg                                                                                    <  1:51805/144‑1 (MQ=255)
                                                                   aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTcc                                                                              >  1:855742/1‑150 (MQ=255)
                                                                   aGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTcc                                                                              >  2:234433/1‑150 (MQ=255)
                                                                     cgcgcCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAg                                                                            >  2:170283/1‑150 (MQ=255)
                                                                         ccACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGggcg                                                                        <  1:155793/150‑1 (MQ=255)
                                                                         ccACCCTTGAAGCCGGCCAGTGGCGCGACGTGAAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTCGTCCAGggcg                                                                        >  1:145068/1‑150 (MQ=255)
                                                                            cccTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCgg                                                                                      <  1:598956/133‑1 (MQ=255)
                                                                            cccTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCgg                                                                                      >  2:598956/1‑133 (MQ=255)
                                                                               ttGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAgg                                                                  <  2:855742/150‑1 (MQ=255)
                                                                                       ggCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAg                                                          <  1:170283/150‑1 (MQ=255)
                                                                                        gCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTTTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGTCGGCCAGGGCGTCCAgg                                                         <  1:304789/150‑1 (MQ=255)
                                                                                        gCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAgg                                                         <  1:897186/150‑1 (MQ=255)
                                                                                               gcgcGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGtt                                                  <  1:380304/150‑1 (MQ=255)
                                                                                                   gACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCAc                                              <  2:188955/150‑1 (MQ=255)
                                                                                                           agaaCTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAg                                      >  1:448812/1‑150 (MQ=255)
                                                                                                            gaaCTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAgg                                     <  1:234433/150‑1 (MQ=255)
                                                                                                             aaCTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGt                                    <  2:545460/150‑1 (MQ=255)
                                                                                                                ttGAAACGCTAGCCAAGCTTTTCGCAGAGGGCCTTGAGCGGCGCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGAGACTGTTTCACAATATCAgataga                                 >  2:279814/1‑145 (MQ=255)
                                                                                                                        cTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTa                         <  2:920637/150‑1 (MQ=255)
                                                                                                                            ccAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCAc                     >  1:1047641/1‑150 (MQ=255)
                                                                                                                                        gaGTGGGCCGTGGGAGGATCACACGGGCCCGGGTCCTTGGTGTTGTTGTCGATCACCCGCACTTCGTAGTCGGGTTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCAcc         <  2:342440/149‑1 (MQ=255)
                                                                                                                                           tGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGac      <  1:739716/150‑1 (MQ=255)
                                                                                                                                               ccTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCt  >  2:204021/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCAGTAGTCCGAGTCGATTACCGCGATCACTTCGGCGTCCTTTGCCGTGTGCGGGATCAGGTAATTCAGCGCGCCACCCTTGAAGCCGGCCAGTGGCGCGACGTGGAAGAACTTGAAGCGCTCGCCAAGCTTTTCGCAGTGGGCCTTGAGCGGCTCCCACACGGCCGGGTCCTTGGTGTTGTTGTCGATCACCAGCACTTCGTAGTCGGGGTAGTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCT  >  NC_002947/1732355‑1732647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑