| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,019,126 | C→A | 18.0% | L120M (CTG→ATG) ‡ | PP_1797 → | HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,019,126 | 0 | C | A | 18.0% | 86.1 / 12.3 | 39 | L120M (CTG→ATG) ‡ | PP_1797 | HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base C (12/20); new base A (4/3); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01 | |||||||||||
GGGTGGCATCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAA > NC_002947/2018978‑2019271 | gggTGGCATCGTCAAAGAGCTGCATGCCAGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCt < 1:935057/150‑1 (MQ=255) tCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGcctctt < 2:248180/150‑5 (MQ=255) aaaGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGcctct < 2:699949/147‑4 (MQ=255) aaaGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGcctct > 1:699949/1‑144 (MQ=255) aaGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTg < 2:665748/150‑1 (MQ=255) tGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCt > 2:912475/1‑150 (MQ=255) cATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGTAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTg < 2:1109099/150‑1 (MQ=255) tGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTNGGa < 2:704272/150‑1 (MQ=255) aCCTGGTGAAGAAGGGCGATCCGCTGCTTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCgg < 1:504000/150‑1 (MQ=255) aCCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCgg < 2:316546/150‑1 (MQ=255) tGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGa > 2:1005818/1‑150 (MQ=255) gTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATa > 1:930681/1‑150 (MQ=255) gaagaaGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGATGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAAt < 2:830295/150‑1 (MQ=255) aagGGCGATCCGCTGATTGTGCTGGATGAGCAGCCGAGCATGGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGaa < 2:1112310/150‑1 (MQ=255) tCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGa < 1:820790/150‑1 (MQ=255) cTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCt < 2:930681/150‑1 (MQ=255) gATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGa < 1:496828/150‑1 (MQ=255) gATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaa < 2:337932/150‑1 (MQ=255) tCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAgcg > 1:963836/1‑150 (MQ=255) aGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTgg < 2:881538/150‑1 (MQ=255) gCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGc < 2:845450/150‑1 (MQ=255) gTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAgcagca < 1:418022/150‑1 (MQ=255) tATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGcagcag < 1:708972/150‑1 (MQ=255) tATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGcagcag > 1:645016/1‑150 (MQ=255) tGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGCCGTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGgt < 1:912475/150‑1 (MQ=255) gAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGgtg > 1:79806/1‑150 (MQ=255) tGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGAtt > 2:493500/1‑150 (MQ=255) aTTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCg < 2:693339/150‑1 (MQ=255) cGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACTGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGc > 2:714968/1‑150 (MQ=255) acgctcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAGGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTg > 2:339572/7‑142 (MQ=255) acgctcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAGGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTg < 1:339572/136‑1 (MQ=255) tcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg > 1:194657/3‑150 (MQ=255) cggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATACAGGCAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCgg > 2:706038/2‑150 (MQ=255) agGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCgg > 1:233643/1‑150 (MQ=255) agGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCgg < 1:657143/150‑1 (MQ=255) gCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACGGATCGCAAGAAGCGCTGGCAGGTGAAAAGGGGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTc > 1:625350/1‑150 (MQ=255) gCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTc > 1:189264/1‑150 (MQ=255) aCGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCaa < 1:282780/150‑1 (MQ=255) aCGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCaa > 2:657028/1‑150 (MQ=255) | GGGTGGCATCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAA > NC_002947/2018978‑2019271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |