| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,092,423 | G→A | 13.5% | intergenic (+26/+65) | deaD → / ← ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,092,423 | 0 | G | A | 13.5% | 125.7 / 8.6 | 52 | intergenic (+26/+65) | deaD/ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base G (19/25); new base A (3/4); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGG > NC_002947/2092284‑2092572 | agcttcagcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCgg < 1:608103/150‑1 (MQ=255)agcttcagcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCgg < 2:747026/150‑1 (MQ=255) gcttcagcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCggg < 1:554942/150‑1 (MQ=255) gcttcagcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCggg > 1:476643/1‑150 (MQ=255) ttcagctgGGTCGAGCTGCGGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCTTGGCGTAACGAAAAAATCCCTGACCTAAGCCGGGGa < 1:428616/150‑1 (MQ=255) gcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAtttt < 2:476643/150‑1 (MQ=255) cttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGTCTTAGGTCGGGGAttttt > 1:57018/1‑150 (MQ=255) cttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAttttt > 2:806721/1‑150 (MQ=255) cttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGAttttt > 2:806811/1‑150 (MQ=255) aGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc < 2:471872/147‑1 (MQ=255) aGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc > 1:471872/1‑147 (MQ=255) ggCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTcg > 1:511978/1‑150 (MQ=255) tgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGaa < 1:346015/150‑1 (MQ=255) gTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc < 2:754333/116‑1 (MQ=255) gTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc > 1:754333/1‑116 (MQ=255) tCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAaga > 2:520914/1‑150 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAgg < 2:669402/150‑1 (MQ=255) aaGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGc < 1:334652/150‑1 (MQ=255) cAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTc < 1:806811/150‑1 (MQ=255) cAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTc < 1:806721/150‑1 (MQ=255) ggACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGt < 1:76279/150‑1 (MQ=255) cTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTTTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGatc < 2:42348/150‑3 (MQ=255) cTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAAc < 1:573882/150‑1 (MQ=255) tCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAAcc > 2:434983/1‑150 (MQ=255) gcagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGaa > 1:532268/1‑116 (MQ=255) gcagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGaa < 2:532268/116‑1 (MQ=255) gcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCa < 1:594890/150‑1 (MQ=255) gcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCa < 2:57018/150‑1 (MQ=255) cTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAg > 1:209006/1‑150 (MQ=255) tGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAgc > 2:729597/1‑150 (MQ=255) gAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAgcg > 1:470816/1‑150 (MQ=255) tGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACAATTCAGGCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGccc < 1:290556/150‑1 (MQ=255) gTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAgg > 1:669308/1‑150 (MQ=255) gCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc > 2:1039033/1‑66 (MQ=255) gCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc < 1:1039033/66‑1 (MQ=255) cgAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCt < 2:470816/150‑1 (MQ=255) ggATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGaaag < 1:237915/91‑3 (MQ=255) ggATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGaaag > 2:237915/1‑89 (MQ=255) gATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATg < 1:434983/150‑1 (MQ=255) gATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGcacc < 1:1083462/150‑1 (MQ=255) cTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGc > 1:603916/1‑150 (MQ=255) tGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGcc > 1:958654/1‑150 (MQ=255) cGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGaa < 2:209006/150‑1 (MQ=255) gAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGccc > 2:153404/1‑77 (MQ=255) gAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGccc < 1:153404/77‑1 (MQ=255) gAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTATACCTGTACCGGCCCTTTCGCGGGCTTGCCCGGGAAGAGGCCCTTCAGTCGAACCGATAGAAGTCCATCCCCATCCCGCTCAGGAAAAACCACTGATGCACCACCCCGAACTTACt > 2:384780/1‑150 (MQ=255) agaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATg > 1:214441/3‑130 (MQ=39) aaaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATg < 2:214441/130‑1 (MQ=39) aaaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGc < 2:113575/131‑1 (MQ=255) aaaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGc > 1:113575/1‑131 (MQ=39) aaaaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTcc > 2:597373/1‑150 (MQ=255) aaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCg < 1:597373/150‑1 (MQ=255) gCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAgg < 2:603916/150‑1 (MQ=255) | AGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCTTTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGG > NC_002947/2092284‑2092572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |