Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,277,603 C→T 9.3% V340V (GTG→GTA)  PP_2007 ← P‑47‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,277,6030CT 9.3% 108.4 / 3.5 43V340V (GTG→GTA) PP_2007P‑47‑like protein
Reads supporting (aligned to +/- strand):  ref base C (22/17);  new base T (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

TCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGCCCTTATAGTCGACGGTGAT  >  NC_002947/2277455‑2277743
                                                                                                                                                     |                                                                                                                                            
tCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCa                                                                                                                                             >  2:324698/1‑150 (MQ=255)
 ccAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCAc                                                                                                                                            <  1:834230/150‑1 (MQ=255)
  cAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACt                                                                                                                                           >  2:23830/1‑150 (MQ=255)
         cGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGC‑GAGAGGATAAAGGAGATAAAGCCAGGCTTTTGGCGGGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTc                                                                                                                                    >  1:976868/1‑150 (MQ=255)
          gCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCTCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCt                                                                                                                                   <  2:9042/150‑1 (MQ=255)
          gCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCTACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCt                                                                                                                                   <  2:125254/150‑1 (MQ=255)
                gATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTc                                                                                                                             >  2:692707/1‑150 (MQ=255)
                 gtGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGCTGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCg                                                                                                                            <  2:537640/149‑1 (MQ=255)
                 aTGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCg                                                                                                                            >  1:597053/1‑150 (MQ=255)
                            cTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCtt                                                                                                                 <  1:23830/150‑1 (MQ=255)
                                  cGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGaa                                                                                                           <  2:973803/150‑1 (MQ=255)
                                    aGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGt                                                                                                         >  1:71778/1‑150 (MQ=255)
                                        gccACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGAt                                                                                                     <  1:154210/150‑1 (MQ=255)
                                        gccACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGAt                                                                                                     <  2:597053/150‑1 (MQ=255)
                                         ccACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGAGGTGGGTGAT‑CAGGCTGACGATGGGCGAGAACCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACAATTGCTTGACGAAGTTGATg                                                                                                    >  2:354060/1‑150 (MQ=255)
                                             gcgcgGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGgtgt                                                                                                >  1:924780/1‑150 (MQ=255)
                                              cgcgGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGtt                                                                                               <  1:692707/150‑1 (MQ=255)
                                               gcggcgATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATg                                                                                                    <  1:1045648/144‑1 (MQ=255)
                                               gcggcgATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATg                                                                                                    >  2:1045648/1‑144 (MQ=255)
                                                        gcgcTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGc                                                                                     <  2:71778/150‑1 (MQ=255)
                                                                      aTCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGTACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGa                                                                                                      >  1:767425/1‑119 (MQ=255)
                                                                      aTCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGTACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGa                                                                                                      >  1:767263/1‑119 (MQ=255)
                                                                      aTCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGTACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGa                                                                                                      <  2:767425/119‑1 (MQ=255)
                                                                      aTCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGTACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGa                                                                                                      <  2:767263/119‑1 (MQ=255)
                                                                      aTCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCt                                                                       >  1:773665/1‑150 (MQ=255)
                                                                         ggAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGaa                                                                                                                                <  1:806338/90‑1 (MQ=255)
                                                                         ggAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGaa                                                                                                                                >  2:806338/1‑90 (MQ=255)
                                                                         ggAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTcag                                                                    >  2:749044/1‑150 (MQ=255)
                                                                                   gcggcgACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTc                                                          >  2:668975/1‑150 (MQ=255)
                                                                                      gcgACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCAc                                                       >  2:1105649/1‑150 (MQ=255)
                                                                                                  gTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCGAGGTACCAGGGAATATGCACTTGCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATa                                           >  2:799233/1‑150 (MQ=255)
                                                                                                   tGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATAc                                          >  2:470807/1‑150 (MQ=255)
                                                                                                     aT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCt                                        >  1:959491/1‑150 (MQ=255)
                                                                                                     aT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCt                                        >  2:945445/1‑150 (MQ=255)
                                                                                                      t‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTg                                       >  2:549493/1‑150 (MQ=255)
                                                                                                          ggCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGt                                    <  1:668975/150‑1 (MQ=255)
                                                                                                             tGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTGTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCa                                 <  2:490371/150‑1 (MQ=255)
                                                                                                               aCGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAgg                               >  2:1078139/1‑150 (MQ=255)
                                                                                                                          gagCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGcc                    <  2:901912/150‑1 (MQ=255)
                                                                                                                              caggccaggTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGCCCTta                <  1:324698/150‑1 (MQ=255)
                                                                                                                                 gccaggTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGCCCTTATAg             >  1:428071/1‑150 (MQ=255)
                                                                                                                                   caggTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGCCCTTATAGTc           <  1:1078139/150‑1 (MQ=255)
                                                                                                                                            gggAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGCCCTTATAGTCGACGGtgat  >  2:287131/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
TCCAGGACACGCAATCGATGGTGTCGGCCTTGACCGAGCCGCCACGCGCGGCGATGGCGCTGATCAGGTCATCGGAGATCACAGCGGCGACGATGTGGGTGAT‑CAGGCTGACGATGGGCGAGAGCCAGGCCAGGTACCAGGGAATATGCACTTCCTTTCTGAAGTCGGACGATTGCTTGACGAAGTTGATGGTGTTGCCATCGAGCACCAGCTTCATCCTCAGCCAGCCGTTCCACTTCATGTCGATACCTGCGTGCAGGTCACAGTCGCCCTTATAGTCGACGGTGAT  >  NC_002947/2277455‑2277743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑