Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,471,094 T→C 10.8% F206L (TTC→CTC)  PP_2165 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,471,0940TC10.8% 119.4 / 4.7 46F206L (TTC→CTC) PP_2165two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base T (20/21);  new base C (3/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ACCGCCGGTCGCCAGGTGCAGATGAACATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGAAGTGCTCAGCCACATCAAC  >  NC_002947/2470947‑2471240
                                                                                                                                                   |                                                                                                                                                  
cccgccgGTCGCCAGGTGCAGATGAACATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTc                                                                                                                                                  <  2:649362/149‑1 (MQ=255)
aCCGCCGGTCGCCAGGTGCAGATGAACATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTc                                                                                                                                                  <  2:1126305/150‑1 (MQ=255)
  cgccgGGCGCCAGGTGTAGATGAACATGTTACCGACAAGCGCCTGGGATACCGGCGAATTCGCCTTCGAAAAAAAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGACGATTATATCGGCGTTGACGAGCGGCGACTTGGTTTCtc                                                                                                                                                >  2:203584/1‑149 (MQ=255)
   gccgGTCGCCAGGTGCAGATGAACATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTAc                                                                                                                                               <  1:724451/150‑1 (MQ=255)
                    gATGAACATGTTTCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCgg                                                                                                                              <  1:1114570/150‑1 (MQ=255)
                        aaCATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTCTCTACCTCGCCGATGTATCCGGGCAt                                                                                                                          >  2:779760/1‑150 (MQ=255)
                        aaCATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACATTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTCTCTACCTCGCCGATGTATCCGGGCAt                                                                                                                          >  2:224396/1‑150 (MQ=255)
                              ttGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCg                                                                                                                    >  2:816547/1‑150 (MQ=255)
                                        gCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCATTGTCGGCCt                                                                                                          >  2:357322/1‑150 (MQ=255)
                                                 tGGCCGGCGAGTTCGCTTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCttt                                                                                                        <  2:702889/143‑1 (MQ=255)
                                                 tGGCCGGCGAGTTCGCTTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCttt                                                                                                        >  1:702889/1‑143 (MQ=255)
                                                            ttCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCAcc                                                                                                  <  2:636527/138‑1 (MQ=255)
                                                            ttCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCAcc                                                                                                  >  1:636527/1‑138 (MQ=255)
                                                            ttCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGaaa                                                                                      >  1:74298/1‑150 (MQ=255)
                                                            ttCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGaaa                                                                                      >  1:645202/1‑150 (MQ=255)
                                                            ttCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGaaa                                                                                      <  1:233337/150‑1 (MQ=255)
                                                                 cTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCCTCGGCCTTTGGCACCGTCCTGTTGAAATTcct                                                                                 >  2:279353/1‑148 (MQ=255)
                                                                  ttCGAGCACCAGATCAGCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGATGGGCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATg                                                                                >  1:1081367/1‑150 (MQ=255)
                                                                          ccAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCAcgcg                                                                        >  1:177487/1‑150 (MQ=255)
                                                                           cAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACgcgc                                                                       >  2:1065041/1‑150 (MQ=255)
                                                                           cAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACgcgc                                                                       >  2:258603/1‑150 (MQ=255)
                                                                            aGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGcc                                                                      <  1:816547/150‑1 (MQ=255)
                                                                                      gtcgtTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGTCGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGa                                                            <  1:589438/150‑1 (MQ=255)
                                                                                      gtcgtTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGa                                                            <  1:614004/150‑1 (MQ=255)
                                                                                      gtcgtTGTACCTTTCGGGTGATTTTGTCGATTATTTCAGGTGGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGa                                                            <  1:560267/150‑1 (MQ=255)
                                                                                          ttGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCAcgc                                                                         <  1:876916/133‑1 (MQ=255)
                                                                                          ttGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCAcgc                                                                         >  2:876916/1‑133 (MQ=255)
                                                                                              aCCTTTCGGGTGATTTTGTCGATTATTTACGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGGTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAgc                                                    >  2:852386/1‑150 (MQ=255)
                                                                                                cTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAgcgc                                                  >  2:927363/1‑150 (MQ=255)
                                                                                                 tttCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTCTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCgca                                                 <  1:779760/150‑1 (MQ=255)
                                                                                                 tttCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTCTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCgca                                                 <  1:224396/150‑1 (MQ=255)
                                                                                                        tGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAAt                                          <  2:274863/150‑1 (MQ=255)
                                                                                                            tttGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGt                                      <  2:74298/150‑1 (MQ=255)
                                                                                                                tCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGt                                  >  1:172503/1‑150 (MQ=255)
                                                                                                                   attattTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCa                               >  1:231589/1‑150 (MQ=255)
                                                                                                                    ttattTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATTTCCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCaa                              <  1:357322/150‑1 (MQ=255)
                                                                                                                     tattTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAg                             <  1:279353/150‑1 (MQ=255)
                                                                                                                     gattTCCGCGTGGCCGAGAGGCGAATTGCTTTCTGCCCCGCCGTTGTCTCCGGGACTGGGGCGTCATCGGTCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAg                             <  2:1081367/149‑1 (MQ=255)
                                                                                                                       tttCCGCGTGGACGAGCGGTGCATTGGTCTCTACCTCGCCGATGTATCCGGGCCTGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCAGGCGGCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTCCAAGcc                           >  2:543268/1‑150 (MQ=255)
                                                                                                                           cgcgTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGa                                                            <  2:191263/113‑1 (MQ=255)
                                                                                                                           cgcgTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGa                                                            >  1:191263/1‑113 (MQ=255)
                                                                                                                              gTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGaa                    <  1:927363/150‑1 (MQ=255)
                                                                                                                                 gACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGAAGTg                 >  1:35341/1‑150 (MQ=255)
                                                                                                                                        ggcgCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGAAGTGCTCAGcc          <  1:282165/150‑1 (MQ=255)
                                                                                                                                         gcgcATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGAAGTGCTCAGCca         <  1:511970/150‑1 (MQ=255)
                                                                                                                                                gCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGAAGTGCTCAGCCACATCAAc  >  1:12868/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCGCCGGTCGCCAGGTGCAGATGAACATGTTGCCGGAAAGCCCCTGGGTGGCCGGCGAGTTCGCCTTCGAGCACCAGATCATCCCGTCGTTGTACCTTTCGGGTGATTTTGTCGATTATTTCCGCGTGGACGAGCGGCGCATTGCTTTCTACCTCGCCGATGTATCCGGGCATGGGGCGTCATCGGCCTTTGTCACCGTGCTGTTGAAATTCATGACCACGCGCCTCATGTTCGAGCTCAAGCGCAGCAGAATGCGTGAGTTCAAGCCGTCTGAAGTGCTCAGCCACATCAAC  >  NC_002947/2470947‑2471240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑