Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,565,917 G→T 10.2% R360S (CGT→AGT)  pctB ← methyl‑accepting chemotaxis protein PctB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,565,9170GT10.2% 107.7 / 2.4 39R360S (CGT→AGT) pctBmethyl‑accepting chemotaxis protein PctB
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base T (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GCCGCAGCGACGCTGTTGGTGCGCATGCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGAATCA  >  NC_002947/2565778‑2566066
                                                                                                                                           |                                                                                                                                                     
gCCGCAGCGACGCTGTTGGTGCGCATGCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACaaaa                                                                                                                                             >  2:76140/1‑150 (MQ=255)
     aGCGACGCTGTTGGTGCGCATGCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCAGGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGtt                                                                                                                                        <  1:1058203/150‑1 (MQ=255)
                  gTGCGCATGCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGcc                                                                                                                           >  1:802772/1‑150 (MQ=255)
                  gTGCGCATGCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGcc                                                                                                                           >  1:701154/1‑150 (MQ=255)
                     cgcATGCTCTGTTCTTCAGTCCCGATGACCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGt                                                                                                                        <  1:572972/150‑1 (MQ=255)
                          gCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGcc                                                                                                                   <  2:802772/150‑1 (MQ=255)
                             ctGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGaa                                                                                                                <  2:644173/150‑1 (MQ=255)
                                    tcANACCCGATGATCGACGCATTGGACGCGCTGACGACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACCAGGGAGCCAGTTCGCCGAACTCGTcc                                                                                                         >  2:694164/1‑150 (MQ=255)
                                        aCCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACg                                                                                                                                                       <  1:25766/100‑1 (MQ=255)
                                        aCCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACg                                                                                                                                                       >  2:25766/1‑100 (MQ=255)
                                         cccGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGtt                                                                                                    <  2:668699/150‑1 (MQ=255)
                                          ccGATGATCGCCGCATTGGACGCGCTCACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTc                                                                                                   <  1:573350/150‑1 (MQ=255)
                                          ccGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGAGACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTc                                                                                                   >  2:33843/1‑150 (MQ=255)
                                            gatgatCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTg                                                                                                 >  2:870770/1‑150 (MQ=255)
                                                  cGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGc                                                                                           <  2:916821/150‑1 (MQ=255)
                                                         ttGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAggcgg                                                                                    >  1:112452/1‑150 (MQ=255)
                                                            gACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 <  1:213870/150‑1 (MQ=255)
                                                                cgcTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTaa                                                                             >  1:526448/1‑150 (MQ=255)
                                                                       cACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACTTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 >  2:740446/1‑139 (MQ=255)
                                                                       cACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACTTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 <  1:740446/139‑1 (MQ=255)
                                                                       cACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACTTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 <  1:740565/139‑1 (MQ=255)
                                                                       cACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACTTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 >  2:740565/1‑139 (MQ=255)
                                                                       cACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCAcc                                                                      <  1:613421/150‑1 (MQ=255)
                                                                                cGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGCACGAGGTAGCCAGTTCGCCGAACTCGACCTTGTTCTGCACGGCCAGGCGGCGGGGAAGGCCACCTTCAccccc                                                             >  2:425557/1‑148 (MQ=255)
                                                                                cGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTc                                                             >  2:485936/1‑150 (MQ=255)
                                                                                cGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTc                                                             >  2:425601/1‑150 (MQ=255)
                                                                                  aaaGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCgg                                                           <  1:485936/150‑1 (MQ=255)
                                                                                     ggTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGa                                                        <  1:150525/150‑1 (MQ=255)
                                                                                                  gCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 <  2:301649/112‑1 (MQ=255)
                                                                                                  gCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGcggcgg                                                                                 >  1:301649/1‑112 (MQ=255)
                                                                                                    gggTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGgcgc                                         <  2:778290/150‑1 (MQ=255)
                                                                                                           cGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCAt                                  >  1:341511/1‑150 (MQ=255)
                                                                                                            gAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCAtg                                 >  1:652772/1‑150 (MQ=255)
                                                                                                            gAGGACACGTCGCTGAGCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCCCCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCAtg                                 <  2:438448/150‑1 (MQ=255)
                                                                                                               gACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCAtggtg                              <  2:600176/150‑1 (MQ=255)
                                                                                                                  acTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTc                           >  1:1004671/1‑150 (MQ=255)
                                                                                                                    ttCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAg                         >  1:780344/1‑150 (MQ=255)
                                                                                                                        cggATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCggg                     >  2:188670/3‑150 (MQ=255)
                                                                                                                                    tGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCAcc         >  2:894888/1‑150 (MQ=255)
                                                                                                                                       aTACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGa      >  2:713305/1‑150 (MQ=255)
                                                                                                                                        tACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGaa     >  2:339514/1‑150 (MQ=255)
                                                                                                                                           gTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGAATca  >  1:478047/1‑150 (MQ=255)
                                                                                                                                           gTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGAATca  >  1:739347/1‑150 (MQ=255)
                                                                                                                                           gTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGAATca  >  1:739148/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                     
GCCGCAGCGACGCTGTTGGTGCGCATGCTCTGTTCTTCAGACCCGATGATCGACGCATTGGACGCGCTGACCACTTTCTCCGAAAGGTCATGCACCAGGCGGGTGGCCGAGGACACTTCGCTGATCGAGGCATGGATACGTTCGACAAAACGGTTGAACGAGGTAGCCAGTTCGCCGAACTCGTCCTTGTTCTGCACGGCCAGGCGGCGGGTAAGGTCACCTTCACCCTCGGCGATGTCGCGCATGGCGCGGCCCATGGTGGTCAGCGGGCTCATCAGCACCGGAATCA  >  NC_002947/2565778‑2566066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑