| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 27,584 | A→G | 8.0% | intergenic (‑431/+15) | PP_0022 ← / ← PP_0023 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 27,584 | 0 | A | G | 8.0% | 135.3 / 2.9 | 50 | intergenic (‑431/+15) | PP_0022/PP_0023 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base G (2/2); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01 | |||||||||||
CAGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATGCA > NC_002947/27435‑27730 | cAGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCga > 2:625264/1‑150 (MQ=255) cgtcCGCACTGAAGGGATAACCGATCCGTAGGGAGCTTCAGAATCCATCACGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCtt < 1:172123/150‑1 (MQ=255) tcCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCTCGGGAGCTGCtttt < 1:239559/150‑1 (MQ=255) cTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTa < 1:625264/150‑1 (MQ=255) tGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTAc > 2:278938/1‑150 (MQ=255) aaGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCCAAAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACt > 2:39639/1‑150 (MQ=255) gggATAACCTATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTccc > 1:488370/1‑150 (MQ=255) gggATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTccc > 1:282642/1‑150 (MQ=255) cGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTtg < 2:1127912/150‑1 (MQ=255) gATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTtgt < 2:1128857/150‑1 (MQ=255) cGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGTCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCtt > 2:753663/1‑150 (MQ=255) ggAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGc > 2:881374/1‑147 (MQ=255) ggAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGc < 1:881374/147‑1 (MQ=255) gAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGTCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTtgt < 1:753663/150‑1 (MQ=255) gAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTtgt < 1:1116119/150‑1 (MQ=255) gAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGt < 2:282642/150‑1 (MQ=255) ccaagcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTc > 1:51853/1‑109 (MQ=255) ccaagcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTc < 2:51853/109‑1 (MQ=255) agcGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCNTCt < 2:700031/150‑1 (MQ=255) aCTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCttttt < 1:657021/90‑1 (MQ=255) aCTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCttttt > 2:657021/1‑90 (MQ=255) acgacgCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAgggg > 2:937894/1‑150 (MQ=255) ggTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTggg > 1:230982/1‑107 (MQ=255) ggTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTggg < 2:230982/107‑1 (MQ=255) aTCAGCGAGACGGTCGTGATGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTgg < 2:241961/96‑1 (MQ=255) aTCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTgg > 1:241961/1‑96 (MQ=255) tCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGt > 1:938024/1‑150 (MQ=255) gCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCt < 1:473240/142‑1 (MQ=255) gCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCt > 2:473240/1‑142 (MQ=255) gagaCGGGCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCCGCTTCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACACGCTGCCTTTTGTCCGGa > 2:588288/1‑150 (MQ=255) cGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGc < 1:278938/150‑1 (MQ=255) tGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCtt > 1:631821/1‑150 (MQ=255) gggTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCAt < 1:767554/150‑1 (MQ=255) gTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGTGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTg < 1:1101448/150‑1 (MQ=255) gTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCa > 2:991022/1‑147 (MQ=255) gTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCa < 1:991022/147‑1 (MQ=255) gTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGATTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTg > 2:739491/1‑150 (MQ=255) acaGCCCC‑AAAAAAAAAGCAGCTGCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCtt > 2:551083/1‑150 (MQ=255) cc‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGa < 2:79716/76‑1 (MQ=255) cc‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGa > 1:79716/1‑76 (MQ=255) c‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCt > 2:236279/1‑150 (MQ=255) aaaaaaaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTcc > 2:683493/1‑116 (MQ=255) aaaaaaaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTcc < 1:683493/116‑1 (MQ=255) aaaaaaaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTg < 1:39639/150‑1 (MQ=255) aaaaaaaGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGc < 2:488370/150‑1 (MQ=255) aaaaGCAGCTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTg > 1:476749/1‑150 (MQ=255) aaGCAGCTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGAt > 2:82086/1‑150 (MQ=255) cagcTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCACTTTGGGCCTGGCTTGATGCt > 1:464423/1‑150 (MQ=255) agcTCTCGGGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTc < 1:82086/150‑1 (MQ=255) cTCCCGAGAGCTGCTGTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTGTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCAt < 2:174175/150‑1 (MQ=255) ccGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATGCa > 2:871572/1‑150 (MQ=255) | CAGTCTCGTCGTCCGCACTGAAGGGATAACCGATCCGGAGGTAGCTTCAGAATCCATCAAGCCAAGCGGAAGACTCACGACGCGGTCCGATTGATCAGCGAGACGGTCGTGAGGGTTTGCACACAGCCCC‑AAAAAAAAAGCAGCTCCCGAGAGCTGCTTTTTTCTTACTCCCCCTTTTTGTGCTTGTGGGGTTTCAGGTCCGGATGGTCCTTCTGCTTTTCAGGGGTGACTCGCTGCCTTTTGTCCGGAGTGCCATCTTCATTGGTGCGCTTTGGGCCTGGCTTGATGCTCATGCA > NC_002947/27435‑27730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |