Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,962,409 G→T 7.8% Q210K (CAG→AAG)  PP_2592 ← ferric siderophore ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,962,4090GT 7.8% 157.3 / 2.6 51Q210K (CAG→AAG) PP_2592ferric siderophore ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base T (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

TGTTCTCCGGGTGGGTTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCT  >  NC_002947/2962267‑2962555
                                                                                                                                              |                                                                                                                                                  
tGTTCTCCGGGTGGGTTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGcg                                                                                                                                             >  1:143695/1‑150 (MQ=255)
tGTTCTCCGGGTGGGTTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGcg                                                                                                                                             >  1:52512/1‑150 (MQ=255)
  ttCTCCGGGTGGGTTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGcgac                                                                                                                                           <  2:968143/150‑1 (MQ=255)
           tgggtTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTCGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGAAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTc                                                                                                                                  <  1:74493/150‑1 (MQ=255)
              gtTGAAGTAGTTGGACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCGGCAATGCACACGGACACCCTGCATGGCGACGATGCTGTCGGa                                                                                                                               <  2:469025/150‑1 (MQ=255)
              gtTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGGAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGa                                                                                                                               <  1:875480/150‑1 (MQ=255)
              gtTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGa                                                                                                                               <  1:201190/150‑1 (MQ=255)
               ttGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAg                                                                                                                              >  2:1072689/1‑150 (MQ=255)
                                gcggcCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGtt                                                                                                             <  1:988882/150‑1 (MQ=255)
                                   gcCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGAt                                                                                                          <  1:872407/150‑1 (MQ=255)
                                              tgtgGGTGATGTCGAACTCCAGCCCGTACAGCGCGGTCAGCTGTCGCTGGGTCACAACCTGCTCGACCCTGCCGCTGCAATGCCCACCGCCACCCTGCATGGCGACGATGCAGCCCGGGTAGGCAGCTGCCAAATTCATGTCgggacccc                                                                                               >  2:364110/1‑143 (MQ=255)
                                                         tCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTGAGATGGGGCAAAGTTGATGTCGTGCACCACCAAAATCAcc                                                                                    >  1:57866/1‑150 (MQ=255)
                                                         tCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGAGGTCGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCAcc                                                                                    >  1:1031004/1‑150 (MQ=255)
                                                                 cAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGcc                                                                            <  2:528904/150‑1 (MQ=255)
                                                                 cAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGcc                                                                            <  2:727991/150‑1 (MQ=255)
                                                                 cAGCCCGTACAGCGCGTGCAACTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGcc                                                                            >  1:190445/1‑150 (MQ=255)
                                                                  aGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATc                                                                                       <  1:18444/138‑1 (MQ=255)
                                                                  aGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATc                                                                                       >  2:18444/1‑138 (MQ=255)
                                                                        tACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACtcg                                                                     >  1:477083/1‑150 (MQ=255)
                                                                          cAGCGCGTGCAGCTGTCGCTCGGCCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACtcgtc                                                                   <  1:71415/150‑1 (MQ=255)
                                                                           aGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACtcgtcg                                                                  >  1:432566/1‑150 (MQ=255)
                                                                           aGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACtcgtcg                                                                  <  2:1058397/150‑1 (MQ=255)
                                                                            gcgcGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGc                                                                 >  2:1033608/1‑150 (MQ=255)
                                                                                    aGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGa                                                         >  2:1104390/1‑150 (MQ=255)
                                                                                    aGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGa                                                         >  1:65777/1‑150 (MQ=255)
                                                                                            cTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCg                                                          <  2:938353/141‑1 (MQ=255)
                                                                                            cTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCg                                                          >  1:938353/1‑141 (MQ=255)
                                                                                             tCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTTCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGc                                                      >  2:1011301/1‑144 (MQ=255)
                                                                                             tCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTTCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGc                                                      <  1:1011301/144‑1 (MQ=255)
                                                                                             tCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTTCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGc                                                      >  2:1010698/1‑144 (MQ=255)
                                                                                             tCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTTCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGc                                                      <  1:1010698/144‑1 (MQ=255)
                                                                                             tCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGgcg                                                >  1:154351/1‑150 (MQ=255)
                                                                                              cGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAggcg                                                                                                                        >  1:936851/1‑77 (MQ=255)
                                                                                              cGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAggcg                                                                                                                        <  2:936851/77‑1 (MQ=255)
                                                                                              cGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGgcgc                                               <  2:315745/150‑1 (MQ=255)
                                                                                                 tCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCa                                            >  2:910008/1‑150 (MQ=255)
                                                                                                   acaACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATg                                          >  2:893193/1‑150 (MQ=255)
                                                                                                            tCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACg                                 >  1:1043930/1‑150 (MQ=255)
                                                                                                              gACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGt                                                                                                     >  2:425309/1‑80 (MQ=255)
                                                                                                              gACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGt                                                                                                     <  1:425309/80‑1 (MQ=255)
                                                                                                                     ccgctgcACTGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGACGTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGgcgc                        <  2:709947/150‑1 (MQ=255)
                                                                                                                       gctgcAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCAt                      >  1:908780/1‑150 (MQ=255)
                                                                                                                             aTGCACGCGGCCACCCTGCAGGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAAGCACCGGGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAg                <  1:364110/150‑1 (MQ=255)
                                                                                                                             aTGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAg                <  2:1043930/150‑1 (MQ=255)
                                                                                                                                 acacGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGgttg            >  2:423596/1‑150 (MQ=255)
                                                                                                                                  cacGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGgttgt           <  2:52512/150‑1 (MQ=255)
                                                                                                                                   acGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGgttgtt          <  1:660793/150‑1 (MQ=255)
                                                                                                                                         aCCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGGACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACgg    <  2:154351/150‑1 (MQ=255)
                                                                                                                                           ccTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCa                                   <  1:125158/117‑1 (MQ=255)
                                                                                                                                           ccTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCa                                   >  2:125158/1‑117 (MQ=255)
                                                                                                                                           ccTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCt  >  1:759849/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TGTTCTCCGGGTGGGTTGAAGTAGTTGCACAGGCGGCCGCGCGCGCTGTGGGTGATGTCGAAATCCAGCCCGTACAGCGCGTGCAGCTGTCGCTCGGTCACAACCTGCTCGACGCTGCCGCTGCAATGCACACGGCCACCCTGCATGGCGACGATGCAGTCGGAGTAGGCGGCTGCAAAGTTGATGTCGTGCACCACCAAAATCACCGTGCGGCCGAACTCGTCGCACAGCCGACGCAGGGCGCGCATGATCTGCACGGCGTGGCGCATGTCGAGGTTGTTCAACGGCT  >  NC_002947/2962267‑2962555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑