Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,001,867 G→C 7.5% G183A (GGC→GCC)  PP_2625 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,001,8670GC 7.5% 145.9 / 2.4 51G183A (GGC→GCC) PP_2625hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACA  >  NC_002947/3001725‑3002003
                                                                                                                                              |                                                                                                                                        
gCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                   >  2:792982/1‑150 (MQ=255)
gCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGccc                                                                                                                                   >  1:990373/1‑150 (MQ=255)
       cccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCg                                                                                                                            >  2:809746/1‑150 (MQ=255)
        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                           <  1:312881/150‑1 (MQ=255)
        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             >  2:889231/1‑148 (MQ=255)
        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             <  1:889231/148‑1 (MQ=255)
         cGGCTACATCTGATTGCCAGGGGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAg                                                                                                                          <  2:983066/150‑1 (MQ=255)
          ggCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGCGCAGGCCCCGCCCGGCATCGAGc                                                                                                                         <  2:550687/150‑1 (MQ=255)
           gCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCa                                                                                                                        <  2:714895/150‑1 (MQ=255)
                  cTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAgg                                                                                                                 >  1:116089/1‑150 (MQ=255)
                     attaacAGGCGTCCGATCGAGGCAAGCTGGGTCGAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGCGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGCCGGGGCCTGCGCGCCATCGAGCAGATCAGGAAg                                                                                                              >  2:448686/6‑150 (MQ=255)
                      ttGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGc                                                                                                             <  1:364134/150‑1 (MQ=255)
                              ggTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGt                                                                                                     <  2:439186/149‑1 (MQ=255)
                              cGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGTTCAGGAAGCTGATCGGt                                                                                                     <  2:651637/150‑1 (MQ=255)
                                tCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGc                                                                                                   >  1:1108761/1‑150 (MQ=255)
                                         ggCAAGCTGGGTCCAGGTACGCCTGAGCGGGGAGGGGTGGTCTACACGTGAGACGGCCGGGCAAGAGATGGTGGAGGGCCAAGTGCTGGGTGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCGGGAAGCTCATCGGTGCGCAGCGAAt                                                                                          >  2:51316/1‑150 (MQ=255)
                                         ggCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTAt                                                                                          <  1:567568/150‑1 (MQ=255)
                                          gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGCCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGACCGGTGCCCAGCGTATc                                                                                         >  2:163845/1‑150 (MQ=255)
                                          gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                         <  2:389997/150‑1 (MQ=255)
                                            aaGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGa                                                                                       >  1:385209/1‑150 (MQ=255)
                                               cTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGATGt                                                                                    >  2:543513/1‑150 (MQ=255)
                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTTATCGGTGCCCAGCGTATCGAGGTTgtga                                                                               <  1:712076/150‑1 (MQ=255)
                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                               <  2:385209/150‑1 (MQ=255)
                                                       aGGTACGCCTGAGCGGGGAGGGCTGGTCTATCCGTGAGACGCCCTGGTAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtg                                                                            <  1:909739/150‑1 (MQ=255)
                                                             gCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGAAGGAGAACGGCCAAGTGCCCGATGCCGGGCGGGGCATGCACGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTAGCAAGGTTGGGAGTGATCACg                                                                      >  2:266603/1‑150 (MQ=255)
                                                               cTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGAGCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGc                                                                    >  1:10162/1‑150 (MQ=255)
                                                                 gAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGa                                                                  <  1:239952/150‑1 (MQ=255)
                                                                    cGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGt                                                               <  1:576265/150‑1 (MQ=255)
                                                                           ggCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCGGAAAAGCACTTTCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACggg                                                        <  1:809746/150‑1 (MQ=255)
                                                                           ggCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACggg                                                        <  1:792982/150‑1 (MQ=255)
                                                                                gTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGAc                                                   >  1:427922/1‑150 (MQ=255)
                                                                                     cccGTGAGACGCCCTGGCAATTGAAGGAGCCCGGCCAAGTGCTCGATGCCGGCCCGGGCCTGGCCGCCATCGAGCAGATCAGGCAGCTGATCGGTGCCCAGCGCATCGGGCTTGTGAGCGCGCACGGCGAGGTCGACGGGCCGCCCTTCg                                              >  2:517406/1‑150 (MQ=255)
                                                                                     cccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCg                                              >  1:88414/1‑150 (MQ=255)
                                                                                       cGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTAATGGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                            >  2:261891/1‑150 (MQ=255)
                                                                                           agaCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATgcgc                                        >  1:842515/1‑150 (MQ=255)
                                                                                             aCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGAGGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCaa                                      <  2:88414/150‑1 (MQ=255)
                                                                                                   tGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCAGGCCCTTCCAGACAAATATCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTg                                >  2:879564/1‑150 (MQ=255)
                                                                                                    ggCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTgg                               <  2:990373/150‑1 (MQ=255)
                                                                                                           gATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCCGATCGTTGCCCAGCGTATCGAGGTTGTTAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCtt                        <  1:476687/150‑1 (MQ=255)
                                                                                                               gagaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATc                    <  1:30967/150‑1 (MQ=255)
                                                                                                                agaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCa                   >  1:947991/1‑150 (MQ=255)
                                                                                                                agaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCa                   <  2:902720/150‑1 (MQ=255)
                                                                                                                       ccAAGTGCTCGATGTCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGgc            >  1:636213/1‑150 (MQ=255)
                                                                                                                          agggCTCGAGGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTAGCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGcgcg         <  2:234230/147‑1 (MQ=255)
                                                                                                                            tGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGaa       >  2:452190/1‑150 (MQ=255)
                                                                                                                             gCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAg      <  2:842515/150‑1 (MQ=255)
                                                                                                                              cTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGc     >  1:970621/1‑150 (MQ=255)
                                                                                                                               tCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca    >  1:622631/1‑150 (MQ=255)
                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc                <  2:671617/138‑1 (MQ=255)
                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc                >  1:671617/1‑138 (MQ=255)
                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcaca  >  1:761420/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                        
GCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACA  >  NC_002947/3001725‑3002003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑