Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,183,371 G→T 7.1% E330* (GAG→TAG) ‡ PP_2793 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,183,3710GT 7.1% 175.6 / 3.2 56E330* (GAG→TAG) ‡PP_2793acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base T (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGA  >  NC_002947/3183223‑3183512
                                                                                                                                                    |                                                                                                                                             
gggCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGa                                                                                                                                              >  2:865287/1‑150 (MQ=255)
        aCCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCtgt                                                                                                                                      <  1:620958/150‑1 (MQ=255)
          cAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTgg                                                                                                                                    >  2:131943/1‑150 (MQ=255)
           ggggCGTGGGTTTCATGCTGGCCGACAACATGATGGCCCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCTCGGCGCtgatca                                                                                                                                   <  2:153930/149‑5 (MQ=255)
             ggCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCgg                                                                                                                                 <  1:54023/150‑1 (MQ=255)
               cgggggTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTAGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggt                                                                                                                               <  2:531766/147‑1 (MQ=255)
                gTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggtg                                                                                                                              <  1:931946/150‑1 (MQ=255)
                 tGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGggtgg                                                                                                                             <  1:245255/150‑1 (MQ=255)
                     tttCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTAGa                                                                                                                         >  2:1110461/1‑150 (MQ=255)
                      ttCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGAt                                                                                                                        >  2:390498/1‑150 (MQ=255)
                         aTGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACTAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGt                                                                                                                     <  1:131943/150‑1 (MQ=255)
                               gCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGAATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGtt                                                                                                                    <  2:47893/145‑1 (MQ=255)
                               gCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGAATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGtt                                                                                                                    >  1:47893/1‑145 (MQ=255)
                                 cGACAACATGCTCGAGCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCa                                                                                                             >  2:1123299/1‑150 (MQ=255)
                                     aacaTGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTa                                                                                                         >  2:722162/1‑150 (MQ=255)
                                      acaTGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTAt                                                                                                        <  1:1090586/150‑1 (MQ=255)
                                       caTGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTAtt                                                                                                       >  1:170576/1‑150 (MQ=255)
                                        atgatgGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTg                                                                                                      <  2:170576/150‑1 (MQ=255)
                                         tgatgGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGGCCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTgg                                                                                                     <  1:968476/150‑1 (MQ=255)
                                          gatgGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCACGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGGGGTCCATCGTTGCGTCCAGGCATTggg                                                                                                    >  2:194546/1‑150 (MQ=255)
                                           atgGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGt                                                                                                   <  2:377057/150‑1 (MQ=255)
                                               aCCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGAc                                                                                               >  1:835023/1‑150 (MQ=255)
                                                 cTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCTCGGCGCtga                                                                                                                                      <  2:213273/109‑2 (MQ=255)
                                                 cTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCTCGGCGCtga                                                                                                                                      >  1:213273/1‑108 (MQ=255)
                                                 cTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGgc                                                                                                                                           <  2:114100/104‑1 (MQ=255)
                                                 cTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGgc                                                                                                                                           >  1:114100/1‑104 (MQ=255)
                                                    cACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCggg                                                                                          <  1:15254/150‑1 (MQ=255)
                                                      cGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCTGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCTGAGGCGCTGTGGCGGGTGGTCGATCGTAGCGTCCAGGTATTGGGTGGACGCGGGGt                                                                                        <  2:583720/150‑1 (MQ=255)
                                                                      gTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtg                                                                        <  1:548850/150‑1 (MQ=255)
                                                                       tCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGCCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtgg                                                                       >  2:764466/1‑150 (MQ=255)
                                                                         tGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtggtg                                                                     >  1:709410/1‑150 (MQ=255)
                                                                           gCAGTGCGCCTGGGTGCTCGCCCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGa                                                                   <  2:1082416/150‑1 (MQ=255)
                                                                             aCTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCACGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGNCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGc                                                                 <  1:194546/150‑1 (MQ=255)
                                                                              cTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCg                                                                <  1:678607/150‑1 (MQ=255)
                                                                                            tCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtgg                                                                       >  2:396671/1‑129 (MQ=255)
                                                                                            tCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCgtgg                                                                       <  1:396671/129‑1 (MQ=255)
                                                                                               aCCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCggg                                                                                 >  2:86150/1‑116 (MQ=255)
                                                                                               aCCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCggg                                                                                 <  1:86150/116‑1 (MQ=255)
                                                                                                 cAGGGCCGGCGCGCCCATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGCTCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGCCCGGGGCCACCGTGGTGGAGCGGATCTTCCGCGCCATTCGc                                             <  2:220317/150‑1 (MQ=255)
                                                                                                 cAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGAGGGGTGCCCGGGGAAACCGTTGTTGAGCGGATCTTCCGCGACATTCGc                                             <  1:865287/150‑1 (MQ=255)
                                                                                                  aGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGGGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTTGAGCGGCTCTTCCGCGAAATTCGcc                                            >  2:292909/1‑150 (MQ=255)
                                                                                                                  tGTCGATTCGAGCCTGGCCAAGGTGATCAGCGTCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTGTTGGGTGGACGCGGTGTGACCGGGGACACCGTGGTGGTGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGa                            <  2:111411/150‑1 (MQ=255)
                                                                                                                       aTTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTAt                              >  2:589168/1‑143 (MQ=255)
                                                                                                                       aTTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTAt                              <  1:589168/143‑1 (MQ=255)
                                                                                                                               aTGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGaa               <  1:635448/150‑1 (MQ=255)
                                                                                                                                  gCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTg            >  2:1105478/1‑150 (MQ=255)
                                                                                                                                   ccAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGGCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGc           >  2:107959/1‑150 (MQ=255)
                                                                                                                                     aaGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGtt                                                                                                                    >  1:286010/1‑43 (MQ=255)
                                                                                                                                     aaGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGtt                                                                                                                    <  2:286010/43‑1 (MQ=255)
                                                                                                                                          ccacAGCGCCTCGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCAt    >  2:365036/4‑150 (MQ=255)
                                                                                                                                           aTCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGCGCGGATCTTCCCCCACGCCCACTGGCTGCGGATCGAGGACTTCCCGAGCGAAGTGAACCGCATg   >  2:851685/1‑150 (MQ=255)
                                                                                                                                           aTCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATg   >  1:718143/1‑150 (MQ=255)
                                                                                                                                            tCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGa  >  2:421417/1‑150 (MQ=255)
                                                                                                                                            tCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGa  <  1:722162/150‑1 (MQ=255)
                                                                                                                                              aGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGccc                      >  2:653770/1‑128 (MQ=255)
                                                                                                                                              aGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGccc                      <  1:653770/128‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GGGCGAGCACCAGGGCGTGGGTTTCATGCTGGCCGACAACATGATGGACCTGCACGTGGTGCGTCTGGCGGTCTGGCACTGCGCCTGGGTGCTCGACCAGGGCCGGCGCGCCAATGTCGATTCGAGCATGGCCAAGGTGATCAGCGCCGAGGCGCTGTGGCGGGTGGTCGATCGTTGCGTCCAGGTATTGGGTGGACGCGGGGTGACCGGGGACACCGTGGTGGAGCGGATCTTCCGCGACATTCGCCCGTTCCGCATCTATGACGGCCCGAGCGAAGTGCACCGCATGA  >  NC_002947/3183223‑3183512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑